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Biomedical subjects

A Sehgal

Publications and source records attributed to A Sehgal.

At least 127 records · Page 7Linked to original sources
PubMed1985

Horizontal transmission of hepatitis B virus infection in household contacts, Pune, India.

The study was undertaken to detect the risk of infection, if any, among 193 household contacts of 40 hospitalised hepatitis patients (group I) with hepatitis B surface antigen (HBsAg) in their blood. As a control group, 103 household contacts of 27 hospitalised hepatitis patients who were negative for HBsAg (group II) were investigated. The family contacts of the former group had a significantly higher prevalence of HBV infection than those of the latter group (P less than .001). Significant differences were observed both in the prevalence of HBsAg (P less than .05) and antibody to HBsAg (anti-HBs) (P less than .025) between the two groups. IgM antibody to hepatitis B core antigen (anti-Hbc-IgM) was detected in 32 out of the 39 (82%) sera tested from the patients of group I with HBsAg. A statistically significant difference (P less than .005) of HBV prevalence was also found in the contacts of these 32 patients suffering from acute hepatitis B as compared to the contacts of the patients of group II. Overall, the children of the first group showed a significantly higher prevalence of HBsAg as compared to the second group. All the children with HBsAg were positive for HBeAg also, but were negative for anti-HBc-IgM. Anti-HBs was detected in a significantly larger number of adult females. Spouses were found to be affected more than other relatives. A significant difference (P less than .025) was noted in the number of families having HBV markers in group I (80.0%) as compared to those in group II (48.1%).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1984

A sero-epidemiologic study of a water-borne epidemic of viral hepatitis in Kolhapur City, India.

Between February and March 1981 an epidemic of hepatitis occurred in Kolhapur City, Maharashtra State, India. Approximately 1200 cases of jaundice were reported; more than 300 patients were hospitalized and three died. The epidemiological investigations showed a distinct concentration of cases in municipal ward E of the city. Investigations of the ward E water supply system disclosed gross contamination of raw water with sewage at source. The serological studies revealed that the aetiological agent responsible for this epidemic was neither hepatitis A virus nor hepatitis B virus but was likely to have been due to a non-A, non-B hepatitis virus.

Adolescent↗
PubMed1984

Non-A, non-B epidemic hepatitis: visualization of virus-like particles in the stool by immune electron microscopy.

Acute-phase stool samples collected from hepatitis cases during outbreaks of water-borne epidemic hepatitis were examined by immune electron microscopy (IEM). Spherical virus-like particles (27 nm in diameter) were visualized in the stool of a hepatitis patient with serological evidence of non-A, non-B (NANB) hepatitis. The IEM demonstrated serological specificity of the antigen with the patient's own convalescent serum as well as a known pool of NANB hepatitis convalescent sera. It is suggested that these virus-like particles may be the aetiological agent of faeco -orally transmitted NANB epidemic hepatitis in India.

Feces↗
PubMed1983

Steroid metabolism by monkey and human spermatozoa.

Freshly ejaculated spermatozoa from monkey and human were washed and incubated with tritium labelled androgens or estradiol to study the pattern of spermatozoa steroid metabolism. When equal concentrations of steroid substrates were used for incubation, monkey and human spermatozoa showed very similar pattern of steroid conversion. Spermatozoa from both species converted testosterone mainly to androstenedione, but reverse conversion of androstenedione to testosterone was negligible. Estradiol-17 beta was converted mainly to estrone. The close similarity between the spermatozoa of monkey and men in their steroid metabolic pattern indicates that the rhesus monkey could be an useful animal model to study the effect of drugs on the metabolic pattern of human spermatozoa.

Androstenedione↗
PubMedDate not supplied

Molecular changes during the genesis of human gliomas.

Neoplastic transformation in the normal human brain occurs as a result of the accumulation of a series of genetic alterations. These genetic alterations include the loss, gain or amplification of different chromosomes which lead to altered expression of proteins that play important roles in the regulation of cell proliferation. Several common genetic alterations at the chromosomal level (loss of 17p, 13q, 9p, 19, 10, 22q, 18q and amplification of 7 and 12q) have been observed. These alterations lead to changes in the expression of several genes; protein 53 (p53), retinoblastoma (RB), interferon (INF) alpha/beta, cyclic AMP dependent kinase number 2 (CDKN2), mutated in multiple advanced cancers 1 (MMAC1), deleted-in-colon carcinoma (DCC), epidermal growth factor receptor (EGFR), platelet derived growth factor (PDGF), platelet derived growth factor receptor (PDGFR), MDM2, GL1, CDK4 and SAS during the genesis and progression of human gliomas. Recent studies suggest that altered expression of several other genes [MET; MYC; transforming growth factor beta (TGF beta); CD44; vascular endothelial growth factor (VEGF); human neurological-related cell adhesion molecule (hNr-CAM); neuroglial cell adhesion molecule (NCAM L1); p21waf1/Cip1; TRKA; mismatch repair genes (MMR); C4-2; D2-2] and proteins [e.g., cathepsins, tenascin, matrix metalloproteases, tissue inhibitors of metalloproteases, nitric oxide synthase, integrins, interleukin-13 receptor (IL-13R), Connexin43, urokinase-type plasminogen activator receptors (uPARs), extracellular matrix proteins and heat shock proteins] are associated with the genesis of human gliomas. Taken together, these findings point to the accumulation of multiple genetic mutations coupled with extensive changes in gene expression in the etiology of human gliomas.

Brain Neoplasms↗
PubMedDate not supplied

The Flexible Baton TM-12: a case report involving a new police weapon.

The purpose of this case report is to describe a new type of police weapon and the injuries sustained by an individual after being shot with it. The Flexible Baton is a type of ammunition that consists of a fabric bag, filled with lead shot pellets, that is shot from a shotgun. It is predicted that this bag may cause bruising, abrasions, and blunt trauma. In our patient, the fabric bag burst and the pellets penetrated the skin of the elbow, resulting in cortical violation of the distal humerus. As more guns with this ammunition have been introduced throughout the United States, we conclude that injuries from this weapon will be seen with greater frequency.

Adult↗
PubMedDate not supplied

Reliability of a brief outpatient functional outcome assessment measure.

The stability of the musculoskeletal form of the Medical Rehabilitation Follow Along (MRFA) instrument was examined in 47 patients receiving outpatient rehabilitation services. The MRFA instrument was designed to provide information on quality of daily living, including physical function, pain, satisfaction, and emotional/psychological well-being. The instrument consists of thirty questions and can be administered as an interview or a written questionnaire. The MRFA instrument was developed using Rasch analysis procedures and is an extension of previous research involving the Functional Assessment Screening Questionnaire. Forty-seven patients completed the musculoskeletal form of the MRFA on two occasions separated by an interval of 1 to 7 days. The stability of responses was examined using the intraclass correlation coefficient (ICC) and kappa. ICC values for the sections of the MRFA instrument examining quality of daily living and physical functioning ranged from 0.74 to 0.97. ICC values for items assessing pain and feelings of well-being were more variable, ranging from 0.36 to 0.93. The kappa values displayed a similar pattern. The overall stability of the MRFA instrument was found to be adequate for gathering screening information in outpatient settings. Additional research is necessary to confirm the findings of this investigation and extend the results to a larger outpatient population.

Activities of Daily Living↗
PubMedDate not supplied

Association between reproductive tract infections and cervical inflammatory epithelial changes.

OBJECTIVE: To determine the association, if any, between different reproductive tract infections and cervical inflammatory epithelial changes. STUDY DESIGN: Clinical, colposcopic, cytologic, and microbiologic screening for reproductive tract infections was conducted. Subjects were screened for Chlamydia trachomatis, Neisseria. gonorrhoeae, Trichomonas vaginalis, bacterial vaginosis, yeast vaginitis, human papillomavirus, and serology for herpes simplex viruses, syphilis, and human immunodeficiency virus-1 and -2. The subjects were 257 women who visited a maternal and child health center between January 1992 and December 1993. RESULTS: Cervical cytology revealed inflammatory epithelial changes in 207 women (80.5%), the highest proportion in the published series. Inflammatory epithelial changes were significantly associated with the number of reproductive tract infections. Risk increased up to 72.6 fold when women were infected with two or more agents. The positive predictive value of inflammatory epithelial changes for any infection was 88.4%, whereas the negative predictive value for any infection was 63.4%. In univariate analysis, the cervical inflammatory epithelial changes were significantly associated with infections such as chlamydia (28.0%), human papillomavirus (56.5%), Trichomonas vaginalis (18.3%), bacterial vaginosis (38.2%), and herpes simplex virus as evidenced by the presence of immunoglobulin M antibodies (28%). Multivariate analysis, however, revealed an independent association of inflammatory epithelial changes with chlamydia (odds ratio, 21.3; 95% confidence interval, 2.6, 181.3), human papillomavirus (odds ratio, 13.5; 95% confidence interval, 4.5, 39.6), and bacterial vaginosis (odds ratio, 22.6; 95% confidence interval, 2.9, 181) only. CONCLUSION: Cervical inflammatory epithelial changes are significantly associated with reproductive tract infection. Cervical inflammatory epithelial changes predict correctly to an extent of 81% infections with chlamydia, bacterial vaginosis, and human papillomavirus infection. A negative smear, on the other hand, rules out these infections to an extent of 83%.

Adult↗
PubMedDate not supplied

Isolation of differentially expressed cDNAs during ferret tracheal development: application of differential display PCR.

The technique of differential display polymerase chain reaction (DD-PCR) was used to identify cDNA sequences, which are temporally expressed during ferret tracheal airway development. Such differentially expressed cDNAs may ultimately prove to be useful markers in elucidating mechanisms of epithelial differentiation and submucosal gland development in the airway. Using two sets of oligonucleotide primers 15 differentially amplified cDNAs were isolated by comparative reverse transcriptase (RT) PCR of 6-h and 3-day postnatal tracheal poly-A mRNA. In situ hybridization was used to assess the reliability of this method and confirm the differential mRNA expression patterns of cloned cDNAs. Results of in situ hybridization analysis demonstrated that 10 of the 15 cDNA sequences gave a temporally regulated pattern of expression, which was concordant with that of the differential display. Furthermore, sequence analysis of the 15 isolated cDNAs revealed that the majority of clones were amplified from two inverted decamer primers. These findings demonstrate the lack of poly-T priming in the differential display reaction, which suggests that this method may yield substantially more information regarding the coding sequence of cloned genes. In support of this observation, 6 of the 15 cDNA sequences contained one complete open reading frame. Although the majority of cDNAs demonstrated no homology to sequence data bases at the DNA or amino acid level, clone FT-4, which demonstrated a differential expression pattern limited to 3-day tracheal time points, was composed of a 10-amino acid repeat domain that was structurally similar to neuropeptide anthoRFamide and barley D hordein seed protein. A second interesting clone, FT-3, demonstrated an infrequent pattern of expression within a subset of epithelial cells limited to early developmental time points (6 h) and was dramatically reduced by 3 days postnatally. Several additional clones with no homologies to previously cloned genes demonstrated expression patterns that were also temporally regulated throughout tracheal development. Although the function of these temporally regulated genes has not been determined, these genes may ultimately prove to be useful markers of cellular differentiation during tracheal development.

Amino Acid Sequence↗