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Biomedical subjects

A Segal

Publications and source records attributed to A Segal.

At least 109 records · Page 6Linked to original sources
PubMed1986

In vitro transformation of C3H/10T1/2 and NIH/3T3 cells by acrylonitrile and acrylamide.

Acrylonitrile (AN) and acrylamide (AM) are carcinogenic in a number of rodent organs and AN is a suspected human carcinogen. We sought to determine whether AN and/or AM could produce morphological transformation in vitro in C3H/10T1/2 and NIH/3T3 mouse fibroblast cells. Both AN and AM induced a dose-dependent cytotoxic effect in C3H/10T1/2 and NIH/3T3 cells and readily transformed both cell lines. Our conclusions are based on the appearance of cells exhibiting a transformed phenotype and growth in soft agar. AN and AM transformed NIH/3T3 cells to a greater extent than C3H/10T1/2 cells. This is the first reported transformation of cells in vitro by AM.

Acrylamide↗
PubMed1986

Rapid induction of thymic lymphomas by isopropyl methanesulfonate: a preliminary report.

The direct-acting SN1 alkylating agent isopropyl methanesulfonate (IMS) was carcinogenic by subcutaneous injection in female Hsd:(ICR)BR mice, causing thymic lymphoid neoplasms within 7 months in at least 20 of 32 treated mice. No such neoplasms were observed in mice treated with the direct-acting SN2 methyl homolog, methyl methanesulfonate (MMS). Both the IMS-treated mice and the MMS-treated mice initially received 20 mumole of the respective compounds by sc injection once weekly; however, because of toxic effects the dose of IMS was reduced to 10 mumole per injection on the 63rd day and further reduced to 5 mumole per injection on the 120th day, after which this dose was maintained until the 202nd day when the last surviving IMS-treated mouse became moribund and was sacrificed. In 2 of the MMS-treated mice, 93% of which were alive at 288 days, tumors were observed at the site of injection, one being a papilloma and the other a subcutaneous sarcoma. IMS has not previously been implicated as a carcinogen, to our knowledge. Its induction of thymic lymphomas may conceivably be related to its ability to alkylate exocyclic oxygen atoms in the DNA of hemopoietic cells.

Animals↗
PubMed1985

Anatomical basis of the surgical approach to the medial wall of the orbit.

The main neurovascular structures related to the medial wall of the orbit were studied in 70 orbits after injection of the orbital arterial bed with latex dye. The position, anatomical relations and variations of the ophthalmic and ethmoidal arteries and trochlear and nasociliary nerves are described. Some differences were noted in comparison to classical anatomical descriptions, notably regarding the position of the ophthalmic artery which was commonly found to lie medial to the obliquus superior muscle distal to the point where it crosses under the muscle. An important surgical landmark, the lateral orifice of the anterior ethmoidal canal which lodges the anterior ethmoidal artery, was noted and its position described.

Female↗