Biomedical subjects

A Schmassmann

Publications and source records attributed to A Schmassmann.

57 records · Page 4Linked to original sources

PubMed1982

Spermiometrics: objective and reproducible methods for evaluating sperm morphology.

An approach was made to replace the subjective evaluation of human sperm morphology by an objective method applicable in routine laboratories. 20 patients out of infertile marriages (G2 and G3) were compared with 10 normal, fertile men (G1). Two different methods were used. Highly developed instruments and techniques were used in method 1, and cheap and simple ones, designed for routine laboratories, in method 2. Head area, circumference, length, and maximal midpiece width of 100 spermatozoa/subject were determined by a semi-automatic image analysis system. Head variation and average midpiece width were clearly bigger in G2 and G3 than in G1. As a result, formulae were developed, determining the pathological alteration of sperm head and midpiece for the individual semen. In addition, an objective classification into different sperm types was elaborated. Using the developed formulae, results of methods 1 and 2 are well in agreement. Thus, method 2 is recommended for routine laboratories, allowing an objective and reproducible morphology determination within 30 min.

Clinical Laboratory Techniques↗

PubMed1979

Quantification of human sperm morphology and motility by means of semi-automatic image analysis systems.

Sperm morphology and motility are determined using a semi-automatic image analysis system under opto-manual control. The system consists of a microscope equipped with a drawing-tube, a digitizer-tablet, and a cursor interfaced to a small microcomputer. A light-emitting diode is mounted on the cursor, visible as a bright red pilot-light in the microscopic field. Sperm head and midpiece as well as the pathway of motile spermatozoa are traced with the cursor's red pilot light. The microcomputer calculates area, circumference, and length simultaneously. Motility and sperm density are determined altogether using microchamber technique. In a selected group of male subjects with normal, doubtful, and pathological semen analyses biometrical analyses of sperm morphology and motility were performed. Sperm morphology is best described by the variation of the head size and by the average midpiece width. Motility is best described by % motile spermatozoa and mean velocity determined 1 hour after semen collection. Biometrical semen analyses are superior to subjective evaluations regarding degree of information, objectivity, and reproducibility.

Cell Survival↗

PubMedDate not supplied

Deoxyribonucleic acid (DNA) content of morphologically different sperm types from normal and subfertile human males.

20 male patients (G2), affected by idiopathic oligozoospermia, and 10 normal fertile males (G1) were investigated. The DNA content of 50 Feulgen stained sperm heads per subject was determined, using single cell photometry. In a second step area, circumference, length, and width of the same heads were measured, using a semi-automatic image analysis system. Considering only morphologically normal heads, the mean DNA content is only slightly smaller in G2 than in G1. On the contrary, the DNA variation is strongly higher in G2 than in G1. Unfortunately, morphologically defect heads show an increased DNA variation, too. Thus, determining the DNA variation of a semen, containing many morphologically defect heads, a combined determination of the DNA content and head morphology is necessary. This can only be achieved by single cell cytophotometry, which is therefore superior to flow cytophotometry as regards this problem. Many heads with a strongly abnormal DNA content do not show any morphological abnormalities. These subcellular changes can only be detected by the determination of the DNA content.

DNA↗