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Biomedical subjects

A Schaberg

Publications and source records attributed to A Schaberg.

At least 37 records · Page 2Linked to original sources
PubMed1979

On the presence of reverse transcriptase in myelo- and lymphoproliferative disorders.

Buffy coats from 31 patients with a diagnosis of leukemia and 16 normal donors were tested for the presence of a viral-like reverse transcriptase. Eighty-five percent of fresh leukemic buffy coats were positive. Also tested were spleens from 16 patients with hematological disorders and 5 spleens from patients without history of hematological malignancy. The 5 normal spleens were negative. Also negative were 4 spleens from patients with Hairy cell leukemia. From the remaining 12 spleens 7 were positive. Reverse transcriptase measurements can be used to distinguish leukemic from normal buffy coats.

Deoxyguanine Nucleotides↗
PubMed1979

Effect of various routine cytopreparatory techniques on normal urothelial cells and their nuclei.

Nuclear and cytoplasmic sizes of cells in permanent, stained smear preparations differ from those in unfixed unstained cells. In air-dried MGG-stained smears the area of the nucleus is 50% larger and that of the cytoplasm 30% larger. In wet-fixed Papanicolaou-stained smears the nucleus is 10--30% and the cytoplasm is 15--55% smaller. The shrinkage in the wet fixation method is dependent on the concentration of the ethyl alcohol applied. The staining method has relatively little influence on nuclear and cytoplasmic size. The three-dimensional appearance of the smeared, stained cells is also dependent on the cytopreparatory technique applied:in the methods with air-drying the nuclei and cells are flat and in the wet fixation method more spherical. In the methods with air-drying the nuclear:cytoplasmic ratio is larger than that seen with the wet fixation methods.

Cell Nucleus↗
PubMed1978

The efficacy of urinary cytology in the detection of urothelial tumours. Sensitivity and specificity of urinary cytology.

The sensitivity and specificity of urinary cytology in the detection of urothelial tumours using voided urine and applying simple smear preparation and staining techniques have been assessed. Of the 2704 patients under investigation 207 had urothelial tumours. The first urine analysis was positive in 66% of the patients with urothelial carcinoma; an additional 23% of the patients showed positive cytology in repeat smears, resulting in a sensitivity of 89%. The efficacy of urinary cytology depends on the tumour type: for grade 2 tumours, 79% were cytologically positive, 92% of grade 3 and 98% of the grade 4 tumours. The diagnostic efficacy in cases of carcinoma-in-situ, squamous cell carcinoma and adenocarcinoma was comparable with that of grade 4 carcinomas. Grade 0-1 tumours did not result in positive cytology. In eleven cases of lithiasis and in two cases of cyclophosphamide therapy the cytological diagnosis was positive but no neoplasm could be established histologically; these represent true false positive diagnoses. Thus, the false positive rate, 13 out of 165, was 7.88% and the false negative rate, 61 out of 207, was 29.4% when grades 0-1 were included, but 8.75% (14 our of 160) when grades 0-1 were excluded.

Cytological Techniques↗
PubMed1978

Value of cerebrospinal fluid cytology for the diagnosis of malignancies in the central nervous system.

The authors present a retrospective analysis of the results of the cytological examinations of cerebrospinal fluid (CSF) samples and tumor-cyst aspirates deriving from 262 patients treated for malignant intracranial primary and secondary tumors, and vertebral and peridural metastastic processes. Positive preoperative CSF samples were found in 15.3% of all cases of primary cerebral malignancies (13.9% of all gliomas) and positive postoperative CSF samples were found in 40% (91% of the medulloblastoma cases). In all cases of single or multiple secondary cerebral tumors, positive preoperative CSF samples were found in 20%.

Astrocytoma↗
PubMed1977

A new cell line from a human chondrosarcoma.

Morphological and growth characteristics are described of a rapidly growing cell line with epithelioid and giant-cell characteristics derived from a chondrosarcoma in a male patient 65 years of age. This cell line is of considerable interest because in these cells cross-reacting antigens with known animal oncorna-viruses are present. Biochemically, the cells contain particles with a density of 1-16 with "cores" of density 1'23 associated with a reverse-transcriptase-like enzyme and with 70S RNA. Occasionally, virus-like particles were demonstrated by electron microscope in material derived from the culture medium.

Aged↗
PubMed1977

Cytophotometric studies on mycosis fungoides and other cutaneous reticuloses.

Feulgen-DNA cytophotometry was carried out in skin imprint preparations of patients with mycosis fungoides, reticulum-cell sarcoma, lymphomatoid papulosis and Sézary's syndrome. These patients proved to have aneuploid and polyploid DNA histograms. In tumour stage of mycosis fungoides a more bimodal distribution of the DNA values was found. Of 79 cases of suspected malignant reticulosis of the skin, 32 showed an abnormal DNA histogram with 5 percent or more tetraploid and hypertetraploid cells. Thirty of these patients developed a malignant skin reticulosis during the 4-year follow-up period. It is concluded that DNA cytophotometry has value as a supplement to routine morphological histopathology in malignant cutaneous reticulosis.

DNA, Neoplasm↗
PubMed1976

Use of immunohistochemical and morphologic methods for the identification of human growth hormone-producing pituitary adenomas.

Indirect immunoperoxidase and immunofluorescent techniques were used for the detection of growth hormone in routinely processed human pituitary adenomas. With the immunoperoxidase method, distinct immunoreactive cells could be demonstrated in 15 adenomas; all except for one were associated with acromegaly. Immunofluorescence was less useful because of autofluorescence due to the fixation. In histologic stainings, the immunoreactive cells were acidophyl. With immunohistochemistry and electron microscopy, densely granulated and sparsely granulated adenomas could be distinguished. Sparse granulation was associated with an expanded rough-surfaced endoplasmic reticulum and Golgi complex. Clinically, the sparsely granulated adenomas were characterized both by active hormone secretion and aggressive local growth. It is concluded that the immunoperosidase method is of value for functional classification of pituitary adenomas. For information about secretory activity we suggest that electron micrographs of adenoma cells be examined.

Adenoma↗