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Biomedical subjects

A Sattler

Publications and source records attributed to A Sattler.

At least 19 recordsLinked to original sources
PubMed2006

Inhibition of carotis venous bypass graft disease by intraoperative nucleic acid-based therapy in rabbits.

BACKGROUND: Bypass graft stenosis after venous revascularisation procedures is characterised by massive neointimal and vascular smooth muscle cell proliferation triggered via endothelin-1 synthesis in the vessel wall. Decoy oligodesoxynucleotides (ODN) against the transcription factor activator protein-1 (AP-1) inhibits pre-pro-endothelin-1 expression. METHODS: In 20 rabbits, an end-to-side jugular vein bypass to the carotid artery was performed: (group A) 8 grafts were treated with consensus AP-1 decoy ODN, (group B) 8 with mutated control ODN and (group C) 4 received no treatment. Explantation, histomorphometric and immunohistochemical evaluation was performed after 28 days. RESULTS: Median intimal thickness of groups: (A) 28.3 microm, (B) 48.4 microm, (C) 71.1 microm. The decoy ODN-treated group showed a significant reduction of neointima formation ( P = 0.029) and a downregulation of the endothelin receptor. CONCLUSIONS: In this model, neointima formation was reduced by local transfection with consensus decoy ODN against AP-1. Endothelin A and B receptor expression is downregulated. Molecular target nucleic acid-based therapies seem to be a future means of overcoming neointima proliferation in pressure-induced venous graft failure. Intraoperative local application makes it easy to use in routine revascularisation procedures.

Animals↗
PubMed2001

Expression and function of CCAAT/enhancer binding proteinbeta (C/EBPbeta) LAP and LIP isoforms in mouse mammary gland, tumors and cultured mammary epithelial cells.

CCAAT/Enhancer binding proteins (C/EBPs) play important roles in the regulation of cell growth and differentiation. This study investigated the expression and function of C/EBPbeta isoforms in the mouse mammary gland, mammary tumors, and a nontransformed mouse mammary epithelial cell line (HC11). C/EBPbeta mRNA levels are 2-5-fold higher in mouse mammary tumors derived from MMTV/c-neu transgenic mice compared with lactating and involuting mouse mammary gland. The "full-length" 38 kd C/EBPbeta LAP ("Liver-enriched Activator Protein") isoform is the predominant C/EBPbeta protein isoform in mammary tumor whole cell lysates, however, the truncated 20 kd C/EBPbeta LIP ("Liver-enriched Inhibitory Protein") isoform is also present at detectable levels (mean LAP:LIP ratio 5.3:1). The mammary tumor C/EBPbeta LAP:LIP ratio decreases 70% (from 5.3:1 to 1.6:1) when lysate preparation is switched from a rapid whole cell lysis protocol to a multistep nuclear/cytoplasmic fractionation protocol. In contrast to mammary tumors, only the C/EBPbeta LAP isoform is detectable in the mammary gland whole cell and nuclear lysates; the truncated "LIP" isoform is undetectable regardless of isolation protocol. Ectopic over expression of C/EBPbeta LIP or C/EBPbeta LAP did not alter HC11 growth rates. However, C/EBPbeta LIP over expressing HC11 cells (LAP:LIP ratio of approximately 1:1) exhibited a consistent 2-4 h delay in G(0)/S phase transition. C/EBPbeta LIP overexpressing HC11 cells did not express beta-casein mRNA (mammary epithelial cell differentiation marker) in response to lactogenic hormones. This defect in beta-casein expression was not corrected by carrying out the differentiation protocol in the presence of an artificial extracellular matrix. These results demonstrate that the "full-length" C/EBPbeta LAP isoform is the predominant C/EBPbeta protein isoform expressed in mouse mammary gland in vivo and mouse mammary epithelial cell cultures in vitro. C/EBPbeta LIP detected in mammary tumor lysates may result from in vivo production or ex vivo isolation-induced proteolysis of C/EBPbeta LAP. Ectopic overexpression of C/EBPbeta LIP (LAP:LIP ratio of approximately 1:1) inhibits mammary epithelial cell differentiation (beta-casein expression).

Animals↗
PubMed2000

Strategies to optimize CAD prevention in modern cardiology. The "Marburg CAD Prevention Project".

In recent years we identified numerous cardiovascular risk factors and had been able to reduce cardiovascular events by a variety of different interventions. There is no question that we can improve the course of coronary artery disease (CAD) in patients with a high-risk profile by modifying these factors. Despite this knowledge, many patients with known CAD or myocardial infarction are not treated for secondary prevention as recommended by well established guidelines (http:¿www.chd-taskforce.com). In order to improve secondary prevention in our patients we started a project, the so called "Marburg CAD Prevention Project". By this we combine our computer data base of the CAD preventional routine laboratory with the computer program CARDDAS. Individual risk factors and the angiographic findings were analyzed. Patients as well as local physicians were informed on the need to treat the important risk factors. This approach ensures that at least all of our patients with documented CAD receive the appropriate preventional recommendations and treatment.

Adolescent↗
PubMed1999

Lifibrol enhances the low density lipoprotein apolipoprotein B-100 turnover in patients with hypercholesterolemia and mixed hyperlipidemia.

Lifibrol (4-(4'-tert-butylphenyl)-1-(4'carboxyphenoxy)-2-butanol), a new hypocholesterolemic drug, effectively reduces total cholesterol (CH), low density lipoprotein (LDL)-CH, and apolipoprotein (apo) B in experimental animals and in humans. The impact of Lifibrol on the metabolism of apoB-100 containing lipoproteins in patients with hyperlipoproteinemia using endogenous labeling with stable isotopes is examined. Kinetic studies were performed in four male hypercholesterolemic individuals (type IIa) before and on treatment with 450 mg of Lifibrol daily for 4 weeks, and in five male individuals suffering from mixed hyperlipidemia (type IIb) before and on therapy for 12 weeks. Kinetic parameters were estimated by multicompartmental modeling. Lifibrol therapy reduced total CH by 16% (P = 0.012) in all patients, increased triglycerides (TG) by 11% (not significant) in type IIa patients and decreased TG by 34% (P = 0.059) in type IIb patients. During Lifibrol therapy, LDL apoB-100 concentrations decreased by 19% (P = 0.011) in all patients. The decrease in LDL apoB concentrations with Lifibrol therapy was due to an overall increase (75%, P = 0.006) of the fractional catabolic rates (FCR) of LDL apoB. This increase was partially attenuated by a 33% increase in LDL apoB production rate (PR) (P = 0.041). The overall production of apoB increased only slightly. Our data suggest that the major mechanism by which Lifibrol lowers LDL-CH is an increase in receptor-mediated catabolism of LDL rather than a decrease in hepatic apoB production.

Adult↗
PubMed1998

Physico-chemical stability of infusion solutions for epidural administration containing fentanyl and bupivacaine or lidocaine.

This study examines the physico-chemical stability of infusion solutions for epidural administration containing bupivacaine hydrochloride 0.06% or 0.125% or lidocaine hydrochloride 0.25% in 0.9% sodium chloride, each with fentanyl 0.0002%. The solutions were prepared in polyvinyl chloride (PVC) infusion bags and stored without overwrap at room temperature (25-30 degrees C) or refrigerated (4-8 degrees C). Over a period of 32 days stability was determined by visual inspection, pH measurement, and HPLC assay of drug concentrations. Admixtures of bupivacaine/fentanyl and lidocaine/fentanyl proved to be chemically stable over a 32 day period, but physical incompatibility (sorption) with PVC-bags was discovered. The stability of the admixtures was influenced by pH and storage temperature. In none of the tested admixtures with an initial pH value lower than 6, did the concentrations of fentanyl or the local anesthetic decrease under 90% of the initial concentrations. A solution of fentanyl and lidocaine with an initial pH of 6.7 exhibited a rapid decrease of drug concentrations. Supposing fentanyl loss was due to sorption, buffered single drug fentanyl solutions of pH 5.5, 5.8, 6.3, and 6.7 were prepared in glass and PVC containers and stored under the same conditions. All solutions in PVC bags showed relevant fentanyl loss which was more evident at higher pH, whereas fentanyl concentration remained unchanged in glass containers at any of the tested pH values.

Analgesics, Opioid↗
PubMed1997

[Postoperative epidural analgesia with Bupivacaine-HCl 0.06%/Fentanyl 0.0002%. Experiences and physicochemical stability in infusion bags].

Continuous epidural infusion of bupivacaine with the opioid fentanyl represents an effective analgesic method in the therapy of strong postoperative pain after major surgery. Preparation of the required infusion solution in syringes with a volume of 50 ml immediately prior to administration is routinely performed by nursing staff in Germany. The effort required for the preparation is associated with logistical and pharmaceutical difficulties. The preparation of a mixture of bupivacaine hydrochloride 0.06% and fentanyl 0.0002% in 250 ml infusion bags at the pharmacy of the University Hospital Mainz is described. To determine the physicochemical stability, the concentration of bupivacaine-HCl and fentanyl was assessed using HPLC over a period of 32 days; in addition the pH values were determined. After 32 days 95% of the bupivacaine hydrochloride and fentanyl baseline values were recorded. The pH baseline value had decreased from 5.48-5.52 to 0.5-0.7 units. The measured values confirm the physicochemical stability of the mixture of bupivacaine hydrochloride 0.06% and fentanyl 0.0002% over a period of 32 days. The infusion bag can be stored for 4 weeks at room temperature.

English Abstract↗
PubMed1995

Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance.

Plants can recognize pathogens through the action of disease resistance (R) genes, which confer resistance to pathogens expressing unique corresponding avirulence (avr) genes. The molecular basis of this gene-for-gene specificity is unknown. The Arabidopsis thaliana RPM1 gene enables dual specificity to pathogens expressing either of two unrelated Pseudomonas syringae avr genes. Despite this function, RPM1 encodes a protein sharing molecular features with recently described single-specificity R genes. Surprisingly, RPM1 is lacking from naturally occurring, disease-susceptible Arabidopsis accessions.

Amino Acid Sequence↗
PubMed1995

Development of a HPLC-system for quantitative measurement of lidocaine and bupivacaine in patients plasma during postoperative epidural pain therapy.

A HPLC method was developed for the simultaneous quantitative analysis of lidocaine and bupivacaine in plasma, with bupivacaine serving as the internal standard for the assessment of lidocaine and vice versa. The samples are prepared by diethyl ether-extraction of the alkalified plasma and re-extraction using diluted sulphuric acid. This allows the elimination of interfering medication and plasma proteins. The prepared samples are chromatographed with a Merck LiChroCART Superspher 60 RP-select B cartridge column, the local anesthetics are detected using UV-photometry and the concentration is calculated by comparing the peak areas of the analyzed substance and the internal standard. Using a sample volume of 1 ml plasma, concentrations of approximately 2.5 micrograms/ml and 1 microgram/ml can be analyzed with a 95%-confidence interval of 2.5% or 5%, respectively. At higher or lower concentrations, accurate results can be obtained using smaller or larger plasma samples. The evolved analytical method allows the rapid and simple determination of lidocaine and bupivacaine plasma levels at a wide range of concentrations. It is suitable for research purposes as well as for routine analyses.

Analgesia, Epidural↗
PubMed1994

A disease resistance gene in Arabidopsis with specificity for two different pathogen avirulence genes.

The RPS3 and RPM1 disease resistance loci of Arabidopsis confer resistance to Pseudomonas syringae strains that carry the avirulence genes avrB and avrRpm1, respectively. We have previously shown that RPS3 and RPM1 are closely linked genetically. Here, we show that RPS3 and RPM1 are in fact the same gene. We screened a mutagenized Arabidopsis population with a P. syringae strain carrying avrB and found 12 susceptible mutants. All 12 mutants were also susceptible to an isogenic strain carrying avrRpm1, indicating a loss of both RPS3 and RPM1 functions. No mutants were recovered that lost only RPS3 function. Genetic analysis of four independent mutants revealed that the lesions were in RPS3. Thus, a single gene in Arabidopsis confers resistance that is specific to two distinct pathogen avirulence genes--a gene-for-genes interaction. This observation suggests that the RPS3/RPM1 gene product can bind multiple pathogen ligands, or alternatively, that it does not function as a receptor.

Arabidopsis↗
PubMed1981

[Thoracoscopy: therapeutic value in emergency pleuro-pulmonary syndromes and diagnostic value (author's transl)].

Thoracoscopy, a diagnostic method of high precision, may be considered to be a lifesaving gesture in certain dramatic situations. It offers the possibility of a precise diagnosis and effective therapeutic measures, which may be performed endoscopically. Such dramatic situations are represented above all by haemopneumothorax, high tension spontaneous pneumothorax and fulminating pleural effusions, as in Meigs syndrome. Pathology involved may range from traumatic, degenerative or inflammatory conditions to tumours of pathognomic malformations. A number of clinical cases spectacular by virtue of their clinical features, endoscopic diagnosis and successful treatment in dramatic circumstances, are presented.

Adult↗
PubMed1975

The diumycin complex. Comparative studies on antibiotics from diumycin-and macarbomycin-fermentations.

Six phosphorus-containing antibiotics were isolated from both diumycin and macarbomycin fermentation products. On the basis of their chromatographic behavior and of their physico-chemical and microbiological properties it can be assumed that not only the main component but also the five minor components are the same in both antibiotic complexes. A comparison of the six components with the known classifications of diumycins and macarbomycins was made.

Anti-Bacterial Agents↗
PubMed1970

[Further facts in pleural recurrence prevention by radio-colloidal gold in operated breast cancer].

The communication deals with endoscopical facts making recognizable that the intrapleural incorporation of colloidal radio-gold after the so-called radical mastectomy corresponds to a preventive-curative act. There is an analysis and proof of lymphogenous nature of the threatening, in it's manifest stage always lethal early and late pleural metastasis. The special and various features of the pleural cavity in postoperative endoscopy are demonstrated as a basis for the following dosage of radio-gold in a preventive-curative sense. Original, spectacular pictures are represented. On the basis of an experience for decades and because of the insufficient results of surgical therapy and conventional radiotherapy, unsatisfactory quoad sanationem et vitam, it must be pointed out that an additional, intrapleural radio-gold therapy is imperative. The application must be done early after operation. The effectiveness of this application is beyond question, since the pioneer-work of J. H. Müller and the ten years results with his preoperative radio-gold infiltration of the breast and my own endoscopically developed intrapleural radio-gold infusion. Both methods make possible true healing and ten years survival. For this reasons I recommend the employ of the more practicable intrapleural infusion on a big collective. The question of a long-term effectiveness of the therapy with fast electrons (betatron) is still to be decided on the ten years parameter. But I don't doubt that radio-gold is superior to all kind of radiotherapy controlling the contralateral pleural dissemination.

Adenocarcinoma, Scirrhous↗