Search PubMed⌕ Search

Biomedical subjects

A Sakai

Publications and source records attributed to A Sakai.

At least 541 records · Page 30Linked to original sources
PubMed1974

Phospholipid degradation in frozen plant cells associated with freezing injury.

A striking degradation of phosphatidylcholine into phosphatidic acid was observed in the cortical tissues of less hardy poplar (Poplus euramericani cv. gelrica), when the tissues were frozen below a lethal temperature. No change in phospholipids was detected during freezing or even after thawing in the cortical tissues of hardy poplar which survived slow freezing to -30 C or even immersion in liquid N(2) after prefreezing to -50 C. The degradation of phosphatidylcholine during freezing appears to be intimately associated with freezing injury.

Journal Article↗
PubMed1974

Survival of Suspension-cultured Sycamore Cells Cooled to the Temperature of Liquid Nitrogen.

Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) which were immersed in liquid nitrogen after prefreezing to the temperatures from -30 to -50 C in the presence of dimethylsulfoxide and glucose as cryoprotective additive could proliferate vigorously when rewarmed rapidly in water at 40 C. For maintaining high viability of the cells after immersion in liquid nitrogen, it seems to be essential to use the cells at the later lag phase or the early cell division phase. This study provides a possibility for long term preservation in liquid nitrogen of plant-cultured lines.

Journal Article↗