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Biomedical subjects

A Sakai

Publications and source records attributed to A Sakai.

At least 451 records · Page 25Linked to original sources
PubMed1981

[Quantitative evaluation of serial 201Tl Myocardial scintigrams after stress testing in ischemic heart disease (author's transl)].

Five normal adults and 31 patients with ischemic heart disease were studied by stress test, and 201Tl was injected one min. before the end of the test. Myocardial scintigrams in three views were recorded at 10 min, 1 and 2 hours (phase 0, 1 and 2) after the injection. The myocardial wall was geometrically divided into 5 segments. At the area where the count rate (Cts) was highest in phase 0 scintigrams, the washout rate from phase i to j was defined as WRij = (maxCtsi-maxCtsj)/maxCtsi where Ctsi denoted Cts at phase i in the relevant area. The redistribution index (Rdsij) in each segment was defined as Rdsij = [Ctsi X WRij + (Ctsi - Ctsj)]/Ctsi, where Ctsi denoted Cts at phase i in the segment. The ischemic segment was identified as 70% or less of maxCts 0, while the infarcted segment by VCG in phase 0 scintigram. Mean values of Rds 02 in the normal, ischemic and infarcted segments were -6.1 +/- 5.6, 9.7 +/- 7.0 and 3.5 +/- 7.2%, respectively, and the differences were statistically significant (p less than 0.001). All the 26 patients with anginal except one showed WR 12/WR 01 greater than 1.0. When the peak pressure rate product that attained at the test was 200 or more, it was significantly correlated with WR 02 (r = 0.80). The Redistribution index was helpful in quantitative assessment of the ischemic segments. The finding of WR 12 greater than WR 01 after the stress was reliable in suggesting myocardial ischemia.

Adult↗
PubMed1980

Effects of islet-activating protein (IAP) on blood glucose and plasma insulin in healthy volunteers (phase 1 studies).

Islet-activating protein (IAP) is a new active substance purified from the culture medium of Bordetella pertussis. The active protein possesses a molecular weight of 77,000 and an isoelectric point of pH 7.8. The nature of IAP-action is characterized by enhancement of insulin secretory response to glucose and other stimulants. A single injection of IAP into spontaneous diabetes rats resulted in normalization of their glucose intolerance over a period of a month. Acute and chronic animal toxicity tests showed that LD50 of IAP was 127 micrograms/kg in mice and 144 micrograms/kg in rats. After these animal experiments, phase 1 studies were designed and undertaken to establish dosage, duration of action and other factors. IAP of 0.5 micrograms/kg or 1.0 micrograms/kg did not bring about any serious toxic or adverse effects in five volunteers. On the 4th day of a single injection of IAP, insulin secretory response was proved to be enhanced. Follow-up studies showed that the IAP-action continued over a month or at most two months. Two features of IAP, i.e., the enhancement of insulin secretory response and the long duration of the action, was confirmed in healthy persons as well as in animals. As expected, IAP has a strong antigenic reaction resulting in formation of IgG antibody and possibly IgE antibody. The antigenicity of IAP causes some hindrance to clinical usefulness. For avoidance of anaphylactic reaction, IAP should be given repeatedly with care. The problem concerning antigen-antibody reaction should be overcome as soon as possible before the clinical use of IAP as a medicament.

Adult↗
PubMed1980

Lymphocyte stimulation by allogeneic tissue cells in rats: with special reference to differential survival of skin and kidney allografts.

The ability of isolated skin and kidney cells to activate allogeneic lymphocytes in vitro was studied in the MHC-identical and incompatible rat strains. The preparation of these cells and optimal culture conditions were summarized. 3H-thymidine uptake was strongest with skin epidermal cells and weakest with kidney cells. The results obtained from fractionated cells indicated that both Langerhans (Ia positive) and other cells (Ia negative) of the skin epidermis stimulated lymphocytes equally well and that only glomerulus and whole kidney cells stimulated, whereas cells fom the medulla and cultured epithelium failed to do so. It was suggested that skin epidermal cells possess lymphocyte-activating antigens other than Ia antigens that may be responsible for MHC-identical graft rejection. The weaker reaction by kidney cells and the limited localization of lymphocyte-activating determinants suggest that kidney cells may not express specific alloantigens detectable by the procedure employed; these cells obviously carry only a limited amount of Ia antigens.

Animals↗
PubMed1980

[A study of the quantification of (201)TI myocardial scintigrams of prior myocardial infarctions and their influence on left ventricular function (author's transl)].

(201)TI myocardial scintigraphy was performed on 25 cases of prior myocardial infarction, and based upon phantom experiments, the infarct size was quantitatively analyzed as a defect segment percentage (DS%). DS% was compared to the left cardiac function obtained from myocardial scintigrams, RI angiocardiograms, and chest X-ray films, and the results were examined. There was a high degree of correlation between DS% and LVEF and LVEDV/ m(2) (R=-0.72, 0.84). Where DS% was less than 25%, the left ventricular function was almost normal, and where DS% was greater than 25%, deterioration could clearly be seen. There was a correlation between left ventricular volume from myocardial scintigrams and heart volume from chest X-ray films. The (201)TI uptake in the lung field from myocardial scintigrams increased as the infarct size increased, and the uptake in the upper lungs also increased.

Adult↗
PubMed1979

Occurrence of plant sterols in aquatic vertebrates.

Plant sterols were found by gas liquid chromatography in the sterols of five species of aquatic vertebrates; mackerel (Scomber japonicus), rainbow trout (Salmo gairdnerii), smelt (Osmerus dentex), sardine (Sardinops melanosticta) and chimera (Chimera phantasma). The sterols of chimera liver, sardine flesh and sardine viscera contained 9.0, 2.4 and 3.1% of C28 and C29 sterols in addition to 86.7, 96.6 and 95.2% of cholesterol. The occurrence of norcholestandienol, campesterol, beta-sitosterol and C28 stanol was shown by combined gas chromatography-mass spectrometry. Sperm whale (Physeter catodon) sterols consisted of more than 99% cholesterol with only traces of 22-dehydrocholesterol.

Animals↗