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Biomedical subjects

A Sacchi

Publications and source records attributed to A Sacchi.

At least 127 records · Page 7Linked to original sources
PubMed1980

[Utility of angiography in the diagnosis and localization of hepatic carcinomas].

In the field of malignant primitive hepatic tumours, the use of ultrasonic methods and C.A.T. has made a considerable contribution to diagnosis and topographical localisation of such tumours. In addition, hepatic biopsy supplies the histological diagnosis. However it is only by angiography that it is possible to evaluate the involvement of the lobes and segments, supplying the character of invasiveness or otherwise of the lesion through demonstration of the arterial peduncle, and thus giving the surgeon the essential elements for a therapeutic approach. Furthermore, in the case of a monofocal lesion, it is only angiography that can establish the usefulness or otherwise of surgical therapy (William M. - Richard P. J. - Philip C. G. - Robert C. Lim, 1979). The purpose of the work is to examine the angiographic readings, which allow: 1) a diagnosis of hepatic expansive formation to be made, with definition of its nature (bening or malignant); 2) the growth character of the lesion to be characterised through evaluation of the involvement of the vascular structures.

Carcinoma↗
PubMed1979

In vitro and in vivo selection of two Lewis lung carcinoma cell lines.

Two tumor cell lines adapted to grow in vitro were originated from an explant of lung metastases of Lewis lung carcinoma. These lines differ in their malignancy when reinoculated into syngeneic animals; nevertheless, they do not show any difference for their in vitro clonogenic ability. From these lines 2 in vivo sublines of 3LL carcinoma were developed. The TD50 of the 2 in vivo sublines are different, and both the values obtained are lower than that of the original line. These results are interpreted as a selection of more malignant tumor cell lines.

Animals↗
PubMed1978

Bone changes incident to blade-vent implants.

This research was undertaken to study the dynamics of bone changes in relation to endosseous blade-vent implants with vital labeling technique. Three mandibular premolars were bilaterally extracted in six two years old dogs. Subsequently each healed edentulous space was implanted with a titanium endosteal blade. One blade in each dog had the post extending into the mouth; the other had the post cut at the level of the neck and covered by the mucoperiosteal flap. The animals received I.V. Pyrrolidin-Methyl-Tetracycline and Alizarine Red S labelings at 15 and 30 days intervals. Dogs 1, 3 and 5 were sacrificed on the 97th day; dogs 2, 4 and 6 on the 52nd day. On ground sections of the mandibular bone which included the blades under U.V. light, it was observed that the implants were surrounded by remodeled trabecular bone of cancellous nature containing the labeling lines of Tetracycline (yellow) and Alizarine (red). The bone-metal interface consisted mainly of a thick layer of non mineralized tissue of about 70 micrometer. The labeling results showed that the new bone apposition began early (at least by the 15th day) and continued for a long time, being still present at the moment of the last injection (90th day). No difference was recognized between the bone responses to the implants jutting out and those submerged. This confirms that implant failure is mainly linked to irregular traumatism and inflammatory infiltration which occurs over a more prolonged duration.

Animals↗
PubMed1977

Mitochondrial and cytoplasmic ribosomes from mammalian tissues. Further characterization of ribosomal subunits and validity of buoyant-density methods for determination of the chemical composition and partial specific volume of ribonucleoprotein particles.

1. At 0-4 degrees C mitochondrial ribosomes (55S) dissociate into 39S and 29S subunits after exposure to 300mm-K(+) in the presence of 3.0mm-Mg(2+). When these subunits are placed in a medium containing a lower concentration of K(+) ions (25mm), approx. 75% of the subparticles recombine giving 55S monomers. 2. After negative staining the large subunits (20.3nm width) usually show a roundish profile, whereas the small subunits (12nm width) show an elongated, often bipartite, profile. The dimensions of the 55S ribosomes are 25.5nmx20.0nmx21.0nm, indicating a volume ratio of mitochondrial to cytosol ribosomes of 1:1.5. 3. The 39S and 29S subunits obtained in high-salt media at 0-4 degrees C have a buoyant density of 1.45g/cm(3); from the rRNA content calculated from buoyant density and from the rRNA molecular weights it is confirmed that the two subparticles have weights of 2.0x10(6) daltons and 1.20x10(6) daltons; the weights of the two subunits of cytosol ribosomes are 2.67x10(6) and 1.30x10(6) daltons. 4. The validity of the isodensity-equilibrium-centrifugation methods used to calculate the chemical composition of ribosomes was reinvestigated; it is confirmed that (a) reaction of ribosomal subunits with 6.0% (v/v) formaldehyde at 0 degrees C is sufficient to fix the particles, so that they remain essentially stable after exposure to dodecyl sulphate or centrifugation in CsCl, and (b) the partial specific volume of ribosomal subunits is a simple additive function of the partial specific volumes of RNA and protein. The RNA content is linearly related to buoyant density by the equation RNA (% by wt.)=349.5-(471.2x1/rho(CsCl)), where 1/rho(CsCl)=[unk](RNP) (partial specific volume of ribonucleoprotein). 5. The nucleotide compositions of the two subunit rRNA species of mitochondrial ribosomes from rodents (42% and 43% G+C) are distinctly different from those of cytoplasmic ribosomes.

Animals↗