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Biomedical subjects

A Sabbatini

Publications and source records attributed to A Sabbatini.

53 records · Page 3Linked to original sources
PubMed1984

[Lyophilized amino acids versus solutions of crystallized amino acids in total parenteral nutrition].

The authors considered the possibility, for purposes of total parenteral nutrition, to use lyophilized amino-acids as an alternative to the traditional amino-acid solutions, and resolved to check their nutritional and metabolic effectiveness. For this purpose, they appraised some parameters concerning the proteic metabolism in two homogeneous groups of surgical patients, subjected to T.P.N., one with crystallized amino-acids in solution and the other with lyophilized amino-acids. The supply in glucides and in lipides was identic in both groups. It appears evident from the results that the lyophilized amino-acids not only show the advantage of being administrable in nutritional mixtures with reduced volume, but also represent a valid plastic source, and can surely replace the traditional commercial amino-acid solutions.

Adult↗
PubMed1983

Intravenous feeding in hepatectomized patients.

Ten patients, undergone major hepatic resections, treated with parenteral hyperalimentation in the postoperative period, were studied. Considered parameters were: free plasma amino acid levels and daily nitrogen balance. By comparing the trends of parameters under study with a group of patients, also hepatectomized but not treated with T.P.N., the importance of a high energetic and plastic post-operative intake is outlined. T.P.N. following major hepatic surgery appears particularly useful when the quality of the exogenous Nitrogen intake is carefully selected.

Adult↗
PubMed1983

[Carnitine in postoperative parenteral treatment].

The Authors study the effect of intravenous L-Carnithine on the free plasma amino acid pattern in the post-operative course of a group of 25 selected patients who underwent toracic and abdominal elective surgical procedures. They compared the results with the data from another group of patients, in conditions similar to the former, but who did not receive Carnithine. The results show that Carnithine is able to reduce gluconeogenesis and proteolysis, probably by an improvement of lipolysis; so the Authors believe that Carnithine carries a useful contribution in the post-operative care of the surgical patient.

Adult↗
PubMed1982

[Blood amino acid pattern in thyreopathic patients. (pre- and post-operative analysis)].

On the basis of the effects, demonstrated in vitro, of the thyroid hormones on the amino-acidic metabolism at cellular level, the Authors analysed the aminoacidogram of 15 thyreopathic patients. They remarked significant preoperative modifications of some amino-acids, coming back to standard values after operation. The authors think the preoperative modifications are to be ascribed to thyreopathy, whereas the postoperative ones depend on the surgical trauma.

Adult↗
PubMed1982

[Popliteal aneurysm (therapeutic considerations)].

The authors show a case of popliteal artery aneurysm they had the opportunity to observe. In consideration of the high percentage of surgical failures; recorded in the treatment of the complicate forms, they point out the importance of precocious diagnosis. The Authors think the termino-terminal arterial anastomosis after aneurysmectomy has very limited indications.

Aged↗
PubMedDate not supplied

[New techniques in nutritional surveys and food educational programs: volumetric dietetics for portion size assessment].

Data in literature show that body weight in adults and children seems to be related to portion sizes more than to food choices and people are more reluctant to give up favourite foods than to reduce portions. So several scientific societies in USA have recently emphastzied the need to select appropriate portion sizes. At the same time, studies on cognitive strategies show that the best method to help people to remember how much they eat is visualisation/comparison to tridimensional visual aids. So a new technique for evaluating portion sizes, based on comparison of portions to volume of usual objects (baseball, dice, deck of cards) appeared in the last years, in nutritional surveys and food educational projects. Our team, since 1990, appointed a volumetric assessment based on comparison of foods to the fist, palm, fingers of the examined subject; this system has been experienced with success for a long time both in individual dietetics and in food educational projects or surveys. Since the interest for this new technique is rising, the aim of this review is to introduce the method and to delineate the applications in nutritional surveys or educational projects in national and international literature. This method, simple practical and intelligible, seems to be addressed to a wide development in the future.

Diet↗
PubMedDate not supplied

Use of human chondrocyte cell cultures to identify and characterize reactive antibodies in rheumatoid arthritis sera.

OBJECTIVE: To study the reactivity of rheumatoid arthritis (RA) sera with human chondrocyte populations isolated from normal cartilage and expanded in vitro. METHODS: Human articular chondrocytes were cultured as adherent (non-differentiated) cells on plastic dishes or in suspension (differentiated) on dishes previously coated with a thin layer of 1% agarose. Sera from 28 RA patients and 5 paired synovial fluids were tested on lysates from chondrocytes and fibroblasts as control by immunoblot. Antigen expression on the cell membrane was evaluated by flow cytometry in a few sera. RESULTS: In 9/28 RA sera IgG antibodies specific for chondrocyte antigens (97 kDa, 74 kDa, 67 kDa, 60 kDa, 54 kDa, 48 kDa and 37 kDa) were detected. Twelve sera reacted with proteins expressed both on chondrocytes and fibroblasts and 7 with fibroblasts only; two sera had no reactivity. When lysates from adherent or suspension chondrocytes were compared, RA sera reacted with higher intensity and detected more antigens on chondrocytes cultured in suspension. Flow cytometry assay demonstrated that RA sera are able to recognize antigens expressed on the cell membrane of the human chondrocytes. CONCLUSION: Our data indicate that: a) 32% of the RA sera contain antibodies reactive with antigens expressed exclusively by chondrocytes, but this value rises to 75% if antigens expressed both by chondrocytes and fibroblasts are considered; b) the reactivity of fully differentiated chondrocytes in suspension culture is higher than the reactivity of chondrocytes cultured in monolayer; and c) some of the chondrocyte-specific antigens identified are associated with the chondrocyte membrane. Thus, in vitro cultured chondrocytes may be used to study both the specificity and the biological activity of autoantibodies in RA.

Arthritis, Rheumatoid↗
PubMedDate not supplied

[Postoperative modifications of blood aminoacidemic concentration].

Post-operative plasmatic aminoacid concentrations in 2 groups of patients given surgical treatment for various pathologies have been calculated. In both groups, post-traumatic protein catabolism was expressed in significant changes to the aminoacidemic pattern. It is concluded that aminoacidograms could be used as a guide to rational parenteral post-operative treatment.

Adult↗
PubMedDate not supplied

Alpha-enolase is a renal-specific antigen associated with kidney involvement in mixed cryoglobulinemia.

OBJECTIVE: To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia. METHODS: Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing. RESULTS: Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase. CONCLUSIONS: The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemia patients with renal involvement.

Amino Acid Sequence↗