[Formation of SiO2-induced granulomas in mice of various strains].
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Animals↗
Biomedical subjects
A S Ustinov
Publications and source records attributed to A S Ustinov.
[The role of macrophage suppressor activity in the mechanism of metastasis after tumor resection].
The rates of spontaneous metastasis to the lung (SML) and lymph nodes, cytotoxic and antimetastatic activity (AA) of macrophages in the course of and after resection of transplantable mammary adenocarcinoma MMTI as well as cloned tumor MMTv4 were studied in C3Hf mice. Murine macrophages showed AA in adoptive test in the course of MMTv4 growth and specific cytotoxic activity in tests in vitro. Macrophages from bearers of MMTv4 revealed non-specific cytotoxicity only. After tumor resection, a sharp increase in SML and metastasizing into lymph nodes was matched by a lack of AA and cytotoxic activity on the part of macrophages in mice operated on. In tumor cell recipients, they were found to stimulate metastasis formation. Only 60% of operated mice survived and macrophages obtained from them did not reveal any metastasis-stimulating activity. This activity was further curbed due to injections of indomethacin--an inhibitor of prostaglandin E2 synthesis--or as a result of indomethacin pretreatment of macrophages in vitro. The above data implicate suppressor activity in the explosion of metastatic formation observed after tumor resection. The role of macrophage suppressors and that of of prostaglandin E2 in postsurgical stimulation of metastasis spreading are discussed.
Adenocarcinoma↗
Participation of macrophages in the mechanism mediating the enhancement of metastasis formation after tumour resection.
The number of spontaneous lung metastases and the frequency of metastasis formation in the lymph nodes of mice were studied following the induction of tumour growth by injection of tumour cells. A syngeneic transplantable mammary adenocarcinoma, MMT 1, from C3H/He mice, and a cloned strain, MMTV4, obtained by treating MMT1 cells with 5-azacytidine in vitro, were used. No differences between MMT1 and MMTV4 were detected in the number of spontaneous metastases in the lungs of mice. An in vitro cytotoxic test and an adoptive transfer test were used to measure cytotoxic activity and the antimetastatic activity of spleen macrophages. The macrophages from mice bearing the MMT1 tumour exhibited antimetastatic activity in the adoptive transfer test, and specific and nonspecific cytotoxic activity in the in vitro test. Macrophages from mice carrying the MMTV4 tumour possessed nonspecific cytotoxic activity in vitro but did not exhibit antimetastatic activity in the adoptive transfer test. Tumours were surgically removed 13-15 days after their induction. Two weeks after the MMT1 tumour was resected, an abrupt increase in the number of spontaneous metastases in the lungs and in the lymph nodes was observed, whereas after removal of the MMTV4 tumour there were no changes in the number of lung metastases. When mice with the MMT1 tumour were given the cyclooxygenase inhibitor, indomethacin, in their drinking water, there was a significant decrease in the number of spontaneous lung metastases. Spleen macrophages from mice operated on after injection with MMT1 or MMTV4 did not possess specific cytotoxicity in the in vitro test.(ABSTRACT TRUNCATED AT 250 WORDS)
Adenocarcinoma↗
[The rate of macrophage inclusion in the elimination of tumor cells when administered to syngeneic animals].
A degree of destruction of tumour cells after their inoculation to a syngenic recipient correlates positively with sensitivity to the lytic effect of macrophages. A clear-cut participation of macrophages in elimination of tumours cells can be detected 8 h after their inoculation.
Animals↗
[In vivo lysis of tumor cells of varying degrees of immune resistance].
Mouse tumour cell immune resistance in vitro and in vivo is compared. Cell lysis in the cytotoxic test was estimated in the first case, while the dynamics of elimination of the labelled 125I cells from the organism--in the second case. The lysis of cells in vitro and in vivo was found to correspond to 10 out of 11 studied cell lines of mouse tumours. However in spite of the similarity these methods are not identical, since there were cases when differences between certain cell lines were revealed in vitro but not in vivo, and vice versa.
Animals↗
[Heterogeneity of murine sarcoma cells for transplantability due to their different susceptibilities to the lytic action of macrophages].
Sarcoma was induced in C57BL/6 mice with 20-methylcholanthrene. The sarcoma cells were found to be heterogeneous in their transplantability. The study of 9 clones distinct in this property has shown that heterogeneous transplantability was due to different susceptibility to lytic action of macrophages.
Animals↗
[Heterogeneity of mouse sarcoma cells based on tumorigenicity determined by the different degree of contact inhibition of cell division].
The mouse CBA sarcoma characterized by great cellular heterogeneity was obtained from spontaneously in vitro transformed embryonic fibroblasts. The clones of the given sarcoma distinct in this property were studied. It was shown that the only feature limiting the growth of clones characterized by weak tumorigenicity was contact inhibition of cellular growth. This property is easily estimated by the in vitro methods.
Animals↗
[Cytotoxic antibodies against tumor cells in the blood of intact C3H mice].
Cytotoxic antibodies against mouse mammary tumour cells, L-cells and hepatoma 22a cells have been found in the serum of C3H/f and C3H/He mice over 8 months of age. Analogues antibodies were found in the serum of young and old BALB/c mice, but not in C57BL/6 mice. The cytotoxic activity of antimammary tumour cell serum has been completely abolished by its depletion by renal tissue of syngeneic and allogeneic animals.
Animals↗