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Biomedical subjects

A Rubinstein

Publications and source records attributed to A Rubinstein.

At least 235 records · Page 13Linked to original sources
PubMed1984

Precipitation of apo E-containing lipoproteins by precipitation reagents for apolipoprotein B.

We measured the solubility of apolipoprotein E (apo E) after precipitation, with heparin-Mn2+ or dextran sulfate-Mg2+, of lipoproteins containing apo B. Data from 46 randomly selected subjects suggest that apo E is readily precipitated by dextran sulfate-Mg2+, but that heparin-Mn2+ preferentially precipitates apo E associated with apo B-containing lipoproteins while leaving the apo E-containing fraction of high-density lipoproteins (HDL) in solution. In a more detailed analysis of three subjects, we measured the lipoprotein association of apo E by column chromatography on agarose beads, before and after its precipitation from plasma. This study confirmed the preferential solubility of apo E associated with HDL lipoproteins. Using plasma from two normolipidemic subjects, we maintained the heparin concentration at 1.30 g/L and varied the manganese concentration from 9.2 to 184 mmol/L. A 46 mmol/L concentration best separated apo E-containing HDL from apo B-containing lipoproteins. Thus, at these final concentrations, heparin-Mn2+ appears to precipitate the apo E associated with apo B-containing lipoproteins, leaving soluble most of the apo E associated with lipoproteins of HDL size.

Apolipoprotein A-I↗
PubMed1984

Scleredema adultorum associated with localized myositis.

A 70-year-old man presented with large areas of brawny edema of the skin and neck and weakness of the muscles of the forearm. Biopsy revealed scleredema adultorum with inflammatory changes in the forearm muscle. An electromyogram showed a mixed pattern compatible with myositis.

Aged↗
PubMed1983

Acquired immunodeficiency with reversed T4/T8 ratios in infants born to promiscuous and drug-addicted mothers.

A new syndrome of acquired immunodeficiency has been identified in seven children who were small for gestational age at birth and subsequently have exhibited failure to thrive, lymphadenopathy, parotitis, hepatosplenomegaly, interstitial pneumonia, and recurrent infections. All have a profound cell-mediated immunodeficiency with reversed T4/T8 ratios. Six are hypergammaglobulinemic and one has low IgG levels. The mothers of five of the seven children are sexually promiscuous and/or drug addicts. Three mothers have an immunodeficiency similar to that found in their infants. One of them died at age 33 years with a diagnosis of acquired immunodeficiency syndrome. In five of the children and in three of their mothers, there is evidence of a persistent Epstein-Barr virus (EBV) infection. We speculate that a perinatal or in utero transmission of EBV can induce an "infectious immunodeficiency." The clinical, histopathologic, and immunologic features resemble those described in adult homosexuals and drug addicts.

Acquired Immunodeficiency Syndrome↗
PubMed1983

Multicentric gliomas of brain and spinal cord.

The clinical and histopathological findings in a child suffering from both cerebral and spinal gliomas of differing histological types are described. There was a family history of von Recklinghausen's neurofibromatosis. The possibility of overlooking the symptoms and signs pointing to a spinal cord lesion in the presence of a known intracranial tumour is stressed.

Brain Neoplasms↗
PubMed1983

Rapid healing of diabetic foot ulcers with continuous subcutaneous insulin infusion.

Two patients with inadequately controlled diabetes mellitus had chronic foot ulcers that were refractory to repeated attempts with conventional treatment. In both patients, complete healing of the foot lesions occurred six to eight weeks after continuous subcutaneous insulin infusion was instituted and blood sugar levels had become normal. This suggests that good control of diabetes, as obtainable with continuous subcutaneous insulin infusion, stimulates healing of foot ulcers. Possible reasons for this finding are reviewed.

Adult↗
PubMed1983

Intranasal administration of gentamicin in human subjects.

The effectiveness of gentamicin administered by the intranasal route in healthy human volunteers was studied. Thirty minutes after gentamicin in a solution of 1% sodium glycocholate was administered at 2 mg/kg of body weight, gentamicin concentrations of 2.48 +/- 0.42 mg/ml were present in the sera. Serum concentrations decreased to 0.94 +/- 0.13 mg/ml at 180 min. No serious side effects were observed. There was slight local irritation after a single dose. When administered intranasally without glycocholate, gentamicin was not present in the sera in detectable amounts.

Administration, Intranasal↗
PubMed1983

Lymphoma of cytotoxic/suppressor T cell phenotype (T8) following angioimmunoblastic lymphadenopathy.

A patient with angioimmunoblastic lymphadenopathy was treated with chemotherapy to complete remission for 13 years. A single relapse, self-limited, occurred followed 2 years later by the appearance of a disseminated non-Hodgkin's lymphoma with prominent cutaneous lesions and hypogammaglobulinemia. Cells from the peripheral blood and an affected lymph node were characterized by immunologic studies as deriving from the T8 subset of lymphocytes, suggesting a functional relationship between these suppressor cells and the patient's clinical manifestations.

Aged↗
PubMed1983

Apolipoprotein E-enriched lipoprotein subclasses in normolipidemic subjects.

This study was undertaken to objectively define the lipoprotein association of apoE without ultracentrifugation and represents a description of three distinct apoE-containing lipoprotein (LP) subclasses in normal human plasma. The lipoproteins of whole plasma were fractionated in a continuous manner by molecular sieve chromatography using 4% agarose and the elution profile of apoE was compared to that of apoB, apoA-I, cholesterol, and triglyceride. The data show that all apoE in normal subjects is LP-associated and is confined to three discrete LP subfractions of characteristic size. The largest (fraction I), appears to be a subclass of very low density lipoproteins (VLDL). The other two, however, are distinct in size from the major cholesterol-carrying LP; fraction II is intermediate in size between VLDL and the major apoB-containing lipoprotein, LDL; and fraction III is larger than the major apoA-I-containing lipoproteins, HDL, but smaller than LDL. Parallel chromatography of supernatant and infranatant fractions obtained after ultracentrifugation of serum at each of three different densities, 1.006, 1.019, and 1.063 g/ml confirmed that fraction I is of density less than 1.006 g/ml, but that fractions II and III overlap the conventional density intervals.

Apolipoproteins↗
PubMed1982

An immunologic hypotheses concerning some congenital diseases and malformations.

Congenital diseases such as hyaline membrane disease perhaps may come about by transplacental passage of high titred IgG anti HLA antibodies to a fetal pulmonary tissue. Conceivably transplacental passage of high titred anti HLA antibodies (or as yet unidentified antibodies) may interfere with development of tissues at any stage of embryonic and fetal development where the HLA antigen (or other antigen) are present.

Antibodies↗
PubMed1982

Idiopathic lipogranulomatosis of spleen and periaortic lymph nodes with noncaseating granulomas of liver.

An unusual case of acute febrile illness clinically thought to be a manifestation of either granulomatous hepatitis or Hodgkin's disease is described. The spleen and enlarged periaortic lymph nodes at the level of the spleen revealed multiple noncaseating granulomas containing vacuoles and associated multinucleated giant cells. The lesions appeared to be consistent with lipogranulomas. The liver contained multiple noncaseating granulomas. The clinical history and analyses of tissue samples failed to document the presence of exogenous lipid. There was no evidence in support of a viral, fungal, or bacterial cause for any of the known granuloma-forming diseases. The patient recovered on steroid therapy and has remained well for one year following the onset of the acute illness. The possibility that this case represents lipogranulomatous pseudosarcoid of the spleen and periaortic lymph nodes or a variant of sarcoid cannot be excluded.

Aged↗
PubMed1982

Suppression of maternal lymphocyte mitogenic responses by supernatants from short-term placental cell cultures.

Human placental cells were freed from lymphocyte contamination on discontinuous BSA gradients. Supernatants from short-term cultures of these purified placental cell suspensions were predominantly suppressive for maternal lymphocyte blast transformation to phytohemagglutinin and in the mixed lymphocyte culture. The suppressor factor was nondialyzable and did not contain IgG or human chorionic gonadotropin.

Cells, Cultured↗