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Biomedical subjects

A Roux

Publications and source records attributed to A Roux.

101 records · Page 6Linked to original sources
PubMedDate not supplied

[Simultaneous quantitative determination of amprenavir and indinavir in human plasma by high-performance liquid chromatography].

A reversed-phase high-performance liquid chromatographic assay for the determination of the HIV protease inhibitors amprenavir (Agenerase) and indinavir (Crixivan) in human plasma is described, using a mobile phase consisting of 0.50 M phosphate buffer (adjusted to pH 5,5) - Milli-Q water - acetonitrile (120: 1,080: 800, v/v/v). A solid-phase extraction using C18 extraction columns (Discovery columns 100 mg, 1 ml Supelco) and a liquid-liquid extraction with 0.5 ml hydrogenocarbonate/carbonate buffer (adjusted to pH 10.6) and 6 ml methyl ter-butyl ether have been compared. The liquid-liquid extraction has been chosen to be easier and cheaper. The method has been validated over the range of 60 to 3,000 ng/ml for amprenavir and 20 to 3,000 ng/ml for indinavir using a 0.5 ml sample volume. The specificity, linearity, accuracy and precision have been studied. The limit of detection was respectively for amprenavir and indinavir 15 and 4 ng, and the limit of quantification was 60 and 20 ng/ml. Stability tests under various conditions were performed. This assay can readily be used in a hospital laboratory for the routine monitoring of plasma concentrations of amprenavir in HIV-infected patients. The trough plasma concentrations average has been determined in patients treated by amprenavir and indinavir for seven months.

Anti-HIV Agents↗
PubMedDate not supplied

[Emergency toxicology: analytic protocol applicable to biolobic media in tentative autolysis].

Here is exposed a method to detect and identify, without delay and in biological media, toxic substances generally found when attempting autolysis. It implies: 1; coloured reactions performed directly or after having isolated the substances using organic solvants, 2; thin layer chromatographies of these extractums which are developed and revealed in very precise conditions. Techniques for titration of some drugs are exposed. 253 cases of toxic coma have been studied, and the results of this inquiry analysed, more particularly cases induced by barbiturates.

Autolysis↗