Search PubMed⌕ Search

Biomedical subjects

A Roseto

Publications and source records attributed to A Roseto.

At least 55 records · Page 3Linked to original sources
PubMed1984

Monoclonal antibodies to the major capsid protein of human papillomavirus type 1.

Two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (HPV-1) were isolated. The monoclonal antibodies detected HPV-1 antigens in frozen sections of HPV-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with HPV-1 particles in an immunodiffusion test. The two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54 000, and a minor polypeptide, with molecular weight of 76 000, in both the dissociated viral particles and in the wart extracts.

Antibodies, Monoclonal↗
PubMed1984

Detection of cytomegaloviremia using monoclonal antibodies.

Isolation of cytomegalovirus (CMV) from blood plated on embryonic fibroblasts necessitates a delay of 10-30 days. Using monoclonal antibodies (McAb) we have developed a 96 hour test to diagnose viremia. We produced monoclonal antibodies directed against CMV (strain AD 169). Clone E 13 McAb detected a CMV early antigen in nuclei using indirect immunofluorescence. These monoclonal antibodies recognized reference strains (Davis and Towne) and 20 field isolates of CMV. E 13 McAb were therefore selected for the diagnostic tests. MRC 5 cells grown on coverslips in tubes were inoculated with buffy-coat from 51 blood samples from bone marrow allograft recipients and Guillain Barre polyneuritis, and centrifuged on hour at 4000 g at 36 degrees C. The indirect immunofluorescence test (IIF) was performed using E 13 McAb, 48 and 96 hours post infection. Three viremia were detected within 48 hours and five others within 96 hours. Plasma and buffy-coat were also inoculated onto MRC 5 cells grown in flasks. Flasks were observed daily for CMV cytopathic effect (CPE). CPE appeared within an average of 14 days. When CMV was detected by CPE, the IF tests were always positive within 96 hours after drawing the blood.

Antibodies, Monoclonal↗
PubMed1984

Use of monoclonal antibodies in the diagnosis of chlamydial urethritis and cervicitis.

Isolation of Chlamydia trachomatis (C. trachomatis) in cell culture is the most reliable method for the diagnosis of chlamydial infections. The results are obtained in 48-72 hours. We have produced anti-chlamydial monoclonal antibodies (McAb) and by means of indirect immunofluorescence (IIF) we have developed a rapid diagnostic test. Sixty-six patients, 34 males seen for subacute urethritis authenticated by the presence of polymorphonuclear cells on urethral smears and 32 females presenting with leucorrhea were included in the study. The specimens obtained from urethra and cervix were inoculated on Hela 229 cells for isolation of C. trachomatis. A similar specimen was placed in normal saline containing 2% fetal calf serum. 50 microliters of the saline solution were then placed in wells in IF slides, dried at 37 degrees C and fixed for 30 minutes in acetone at -20 degrees C. Anti-chlamydial McAb and fluorescein labelled mouse anti IgG were used for the rapid IIF test. From 34 cases, we have isolated C. trachomatis from the urethra of 19 males. In these 19 cases, most of the polymorphonuclear cells demonstrated a brilliant cytoplasma revealing the presence of chlamydial antigens. In 25 other cases without isolation, the rapid diagnostic test was positive twice. From the 32 females, C. trachomatis was isolated 7 times. In these 7 cases, the rapid diagnosis IIF test was positive with the same characteristics as noted in the male population. In the 25 other cases without isolation, the test was positive 3 times.

Animals↗
PubMed1983

Isolation and characterization of anti-rotavirus immunoglobulins secreted by cloned hybridoma cell lines.

Five monoclonal hybridoma cell lines secreting antibodies against bovine rotavirus have been produced and four of them characterized by immunostaining of structural polypeptides electrophoretically transferred on to nitrocellulose sheets. Three hybridomas appeared to be directed against the major structural polypeptide (VP39) of the virion. These three monoclonals cross-reacted with the major polypeptide of simian rotavirus and human rotavirus. A fourth hybridoma appeared to react specifically with the high-molecular weight external polypeptide (VP89) and its cleavage products. A cross-reaction was observed with human Wa strain but not with SA11. The fifth hybridoma, even though reacting in an immunofluorescent test, did not show any reactivity by immunostaining. None of the monoclonals neutralized the infectivity of bovine rotavirus.

Antibodies, Monoclonal↗
PubMed1983

The diffuse neuroendocrine (APUD) system.

The bulk of experimental evidence indicates that the APUD series of cells is a distinct system based upon common pathophysiological features. The diffuse nature of this system with elements in the central and peripheral nervous system suggests a more complex interaction of the body's homeostasis than has been established. It is probable that as radioimmunological and radioenzymatic assays become more widely available and standardized, other apudomas will be described. Finally, an understanding of the APUD concept, with its peculiar pluripotential for the production of biogenic amines and peptides, the multicentric nature of the disease and the possibility of multiple cell involvement, is of key importance in managing patients. Studies of the apudomas will also advance the understanding of the normal physiologic interrelationships of the APUD cells.

APUD Cells↗
PubMed1983

[Production of monoclonal antibodies against human cytomegalovirus].

Cell fusion of Sp2/0, a murine myeloma derived non-secreting variant, with splenic lymphocytes from Mice immunized against human cytomegalovirus, has originated two stable hybridomas producing monoclonal antibodies (IgG) which react specifically with antigens that appear early in infectious cycle and remain localized in the cell nucleus.

Animals↗
PubMed1982

[Isolation of cell hybrids secreting specific antibodies against bovine enteric coronavirus (author's transl)].

Twelve hybrid cell lines secreting monoclonal immunoglobulins (IgG) specific of bovine enteric Coronavirus (BEC strain G110) have been obtained following fusion of non secreting variant of the myeloma P3X63Ag8, SP2O, which splenic lymphocytes of Mice immunized against this virus. Antibodies thus obtained have been studied by indirect immunofluorescence and seroneutralization with cells infected by two french strains G110 and F15 and an american strain of Coronavirus: NCDCV. Immunochemical studies have confirmed this specificity.

Animals↗
PubMed1982

Bovine enteric coronavirus structure as studied by a freeze-drying technique.

A strain of bovine coronavirus (F15) was studied by electron microscopy using a freeze-drying technique. Purified coronavirus preparations show three different categories of image: (i) 'blackberry-like' virions, (ii) virions with a smooth depression at their surface, and (iii) apparently broken particles showing very clearly the areas of spike insertion in the virus membrane. Virus projections resemble 'mushrooms' with the 'stalk' inserted at the virus membrane. A model of the virion structure is proposed.

Animals↗
PubMed1982

Serotonin and histamine production by human carcinoid cells in culture.

We are reporting on the first human carcinoid cells ever cultured in vitro. These cells, termed CGP, originated from a jejunal carcinoid tumor. Before tumor resection, the 29-year old male patient presented high levels of blood serotonin and histamine and also of urinary serotonin and 5-hydroxyindole-acetic acid; values returned to normal 9 days after resection. CGP cells exhibit a very slow multiplication rate; generation time is about 10 days. From the first subculture, the main cytological, ultrastructural, and biochemical features of CGP cultures remain unchanged. The cells show most of the enterochromaffin cell histomorphological characteristics; for example, cytoplasmic granulations, specific of argyrophilic cells, can be seen both by electron microscopy and by light microscopy (preceded by silver impregnation). The high amounts of serotonin and histamine found by highly specific radioenzymatic assay in the supernatant of CGP cultures indicate that, after 6 months (25 subcultures), CGP cells have retained the main metabolic characteristics of the original tumor, i.e., the ability to synthesize, store, and release both serotonin and histamine.

Adult↗
PubMed1980

[Presence of enteric viruses in non-diarrheic canine stools].

Rota-, corona- and parvovirus particles have been visualized by direct electron microscopy in canine stools collected at random in Paris streets. A possible involvement of these viruses in gastroenteric diseases is discussed in the light of these findings.

Animals↗
PubMed1980

Electron microscopy detection and characterization of viral particles in dog stools.

A number of apparently normal dog stool samples, randomly collected on the sidewalks of Paris were examined by Electron Microscopy. The study revealed the presence of viral particles in 27 cases. Morphological criteria lead to the characterization of rotavirus in 2 specimen, coronavirus in 7 and parvovirus in 5. Rotavirus particles appeared always alone while coronavirus and parvovirus particles were present together in 5 cases. Similar particles have been implicated in animal and human gastroenteritis. The importance of their presence in canine dejections is discussed in view of pollution by dog stools of urban and suburban areas.

Animals↗