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Biomedical subjects

A Ron

Publications and source records attributed to A Ron.

83 records · Page 5Linked to original sources
PubMed1975

Cells in spontaneous DNA synthesis in cord blood of premature and full-term newborn infants. An autoradiographic study.

Cord blood from 16 premature infants and 10 full-term infants, and blood from 10 healthy adults, was incubated for 30 minutes with tritiated thymidine, after which an autoradiographic study was made of spontaneously labeling cells. Apart from a varying number of erythroblasts, myelocytes, and an occasional blast cell, two main types of spontaneously labeling cells were observed: transitional cells and large lymphoid cells, previously shown to be phagocytic. Spontaneously labeling cells were 12 times more frequent in cord blood of premature and full-term infants than in the blood of adults. No transitional cells were seen in adult blood.

Autoradiography↗
PubMedDate not supplied

Diagnostic test restraint and the specialty consultation.

OBJECT: To assess the effect consultants had on the diagnostic process in the management of patients admitted to the medical service of a university hospital. DESIGN: Cohort study utilizing prospective evaluation by residents, retrospective chart review, and direct communication with the patient, a family member, or the patient's physician one year after admission to the hospital. SETTING: The medical inpatient service of an urban university hospital. PATIENTS: The 580 patients admitted to the medical service during one month in 1984 for whom complete data were available. MAIN RESULTS: Sixty-three percent of the patients had consultations. Seventy percent (198/284) of the patients admitted by generalists had consultations, while 57% (170/296) of the patients admitted by subspecialists had consultations. Of the 1,422 major diagnostic tests performed on these patients, 504 (35%) were first recommended by consultants, and the consultants recommended cancellation of only ten major diagnostic tests. Patients who were seen by consultants had a length of stay that was more than double that of patients not seen by consultants. Consultation was associated with prolonged stay when patients were stratified by important clinical variables and remained an important independent factor in a multivariate model. The prolongation of hospitalization was principally due to delays in scheduling and interpreting sophisticated tests recommended by the consultants. When stratified into prognostically similar clinical groupings, there was no significant difference in in-hospital mortality between patients seen and those not seen by a consultant. CONCLUSION: Efforts to foster diagnostic restraint in the management of hospitalized patients should be broadened to include attention to the specialty consultation process.

Clinical Laboratory Techniques↗
PubMedDate not supplied

The immobilization antigens of Tetrahymena thermophila are glycoproteins.

The four immobilization antigens controlled by the SerH locus in Tetrahymena thermophila have been isolated and partially characterized (Doerder, F.P. & Berkowitz, M.S. 1986. Purification and partial characterization of the H immobilization antigens of Tetrahymena thermophila. J. Protozool., 33:204-208). We show here, using immunoprecipitation and electrophoresis after labeling with 35S-methionine, 14C-mannose, 14C-glucosamine, and N-Acetyl-D-[l-3H]glucosamine, that these proteins are glycosylated. We suggest the immobilization antigens in Tetrahymena may be anchored to the surface membrane by phosphatidylinositol glycans.

Animals↗
PubMedDate not supplied

Quality improvement through review of inpatient deaths.

Emphasis on hospital mortality as a monitoring tool has raised concerns about the validity of mortality rates as a measure of quality of care. An indepth review of all mortalities at Beth Israel Medical Center was conducted from 1988 through 1993. Clinical issues identified from chart review were referred for departmental physician peer review, and quarterly reports of trends and issues were disseminated to all levels of the institution. Mortality rates declined 21% over the 6 years, from 3.3% to 2.6% of all discharges. Clinical quality issues were identified in less than 3% of all mortalities. The majority of problems were related to delays and appropriateness of treatment (57% of quality issues). The review program identified specific hospital processes for improvement, and, more importantly, created a "watchful concern" about quality-of-care issues throughout the hospital.

Centers for Medicare and Medicaid Services, U.S.↗
PubMedDate not supplied

Penalization treatment of amblyopia: a follow-up study of two years in older children.

Penalization therapy for strabismic amblyopia is indicated in the group over six years of age. Good visual acuity of 6/12 or better was achieved and maintained at least for two years in 74% of the 38 cases reported. Deep amblyopia of 6/60 or less is no contraindication to treatment, success being achieved in 50% of this group. It seems that previous occlusion treatment diminishes the rate of success. Close supervision during the treatment is mandatory to assure the desired use of the amblyopic eye.

Amblyopia↗
PubMedDate not supplied

A potential mucus precursor in Tetrahymena wild type and mutant cells.

By using an antibody to a specific mucus polypeptide (34 kDa) to study whole cell extracts of both a secretory mutant (SB281) and wild type (wt) Tetrahymena, we demonstrate that a 57-kDa polypeptide is a probable precursor to the 34-kDa secretory polypeptide. We postulate that the precursor accumulates in the mutant cells because it cannot be cleaved. This mutant contains no recognizable mature secretory granules (mucocysts). By immunoelectron microscopy, the 34-kDa polypeptide was localized in wt cells specifically to the mature mucocysts and to their released products. Localization in mutant cells occurred in two different types of cytoplasmic vesicles: small electron dense vesicles (0.3-0.5 microns in diameter) and large electron lucent vacuoles (1.2-3.5 microns in diameter). Immunoblot analyses of homogenates of mutant and wt cells with the anti-34-kDa serum revealed a dominant band in the mutant at Mr 57 kDa whereas the wt showed a dominant band only at Mr 34 kDa. Furthermore, the 57-kDa polypeptide is immunoprecipitated with anti-34-kDa serum from the mutant cell. Further evidence for a precursor relation of the 57-kDa polypeptide in mutant cells to the 34-kDa mucus polypeptide of wt cells was obtained by the use of drugs (monensin, chloroquine, NH4Cl) that block secretory product processing in wt cells. Extracts of drug-treated wt cells showed the presence of a 57-kDa cross reacting band even after 18 h of incubation in growth medium whereas untreated control cells contained the 34-kDa mature protein almost exclusively. These results indicate that processing of the precursor to the 34-kDa polypeptide occurs in an acidic compartment(s) possibly in either the trans Golgi network, or condensing vacuoles or both.

Animals↗