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Effect of reoxygenation of the serum enzymatic activities and protein fractions in dog with acute myocardial infarction. The enzymatic activitites creatin phosphokinase (CPK; E.C. 2.7.3.2), glutamate oxalacetate transaminase (GOT; E.C. 2.6.1.1.), diamine oxidase (DAO; E.C. 1.4.3.6.), monoamine oxidase (MAO; E.C. 1.4.3.4.), catalase (E.C. 1.2.1.6.), peroxidase (E.C. 1.2.1.6.) and the protein fractions levels are measured in the dog serum during experimental myocardial infarction, followed by 2 hours of reoxygenation. The alpha, and alpha2 globuline serum proteins increase during anoxic period; after reperfusion alpha1 globuline content further increase again, while alpha2 globuline fraction decrease to control values. The enzymatic activities, particulary CPK, DAO and MAO, significantly enhance during coronary occlusion. After riperfusion the DAO, GOT and peroxidase activities decrease, while the CPK, atalase and MAO activities further increase, particulary at the early stage of the reperfusion. These results are discussed interms of cellular mechanism, induced by oxygen readmission.