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Biomedical subjects

A Razin

Publications and source records attributed to A Razin.

105 records · Page 6Linked to original sources
PubMed1973

DNA methylase induced by bacteriophage phiX174.

A cytosine-specific DNA methylase activity, which is normally absent in the Escherichia coli B strain, was found to be induced in these cells by infection with bacteriophage varphiX174. In vivo experiments revealed a single 5-methylcytosine residue in the phage DNA molecule and 5-methylcytosine residues in the infected host DNA, in addition to the 6-methylaminopurine residues present in the uninfected cells. In vitro, a partially purified enzyme from infected cells methylated DNA from uninfected cells, but showed no activity with cellular DNA from infected cells. The partially purified methylase derived from uninfected cells lacks this activity.

Adenine↗
PubMed1970

Replicative form II DNA of phi X174; resistance to exonucleolytic cleavage by E. coli DNA polymerase.

The progeny replicative form II DNA isolated from E. coli cells infected with varphiX174 is resistant to the exonucleolytic activity associated with E. coli DNA polymerase. A limited endonucleolytic cleavage with micrococcal endonuclease renders the replicative form II molecule susceptible to the exonucleolytic activity associated with the E. coli DNA polymerase. These experiments suggest that the resistance of the replicative form II DNA to the exonucleolytic activity is a result of the structure of these molecules at their 5'-terminus.

Chemical Phenomena↗