Search PubMed⌕ Search

Biomedical subjects

A Rapak

Publications and source records attributed to A Rapak.

23 records · Page 2Linked to original sources
PubMed1988

Enzyme-linked immunosorbent assay of basic pancreatic trypsin inhibitor.

Using specific rabbit antibody, one non-competitive (sandwich) and three variants of competitive enzyme-linked immunosorbent assays for basic pancreatic trypsin inhibitor (BPTI) were elaborated. For the assays peroxidase-antibody, two peroxidase-BPTI and beta-galactosidase-BPTI conjugates were applied. The best was competitive assay with peroxidase-BPTI conjugated by two step glutaraldehyde procedure. By this assay we were able to determine 1-10 ng BPTI/ml (0.007-0.07 KIU/ml) and the presence of human blood serum did not affect the assay of the inhibitor.

Animals↗
PubMed1987

The use of acrylic copolymers as antibody carriers for immunoaffinity chromatography.

Among several various acrylic copolymer used as carriers for anti-bovine serum albumin the best are copolymers obtained from acrylonitrile and ethyl or butyl acrylate crosslinked with divinylbenzene which after aminolysis with di- or triamines were activated with glutardialdehyde. The immunoadsorbents thus obtained were successfully used for purification of bovine serum albumin from bovine serum or from crude fraction of the albumin by immunoaffinity chromatography. Electrophoretically pure bovine albumin was eluted from immunoadsorbent column with deionised water and with glycine/HCl buffer, pH 2.5. The albumin was contamined with less than 0.1% of bovine immunoglobulins and with 0.05% of rabbit immunoglobulins. No contamination with rabbit immunoglobulin was observed when immunoadsorbents were pre-treated with sodium borohydride.

Acrylonitrile↗
PubMed1986

The use of normal filter paper for "dot-immunobinding assay" of some antibodies.

Normal filter papers were used for specific detection of tiny amounts of antibodies against two penicillin amidase molecular forms from Escherichia coli, monoclonal antibodies of IgG and IgM class against lipopolysaccharide from Citrobacter O36 and IgM antibody against glycoprotein N from human red blood cells. For colour detection of antibodies bound to proper antigens adsorbed on the paper, second antibody-horse radish peroxidase conjugates and hydrogen peroxide with 4-chloronaphthol were applied. The use of filter paper for dot-immunobinding assay of antibodies gave similar results to those obtained with expensive nitrocellulose sheets used by other authors.

Antibodies↗