Search PubMed⌕ Search

Biomedical subjects

A R Sheth

Publications and source records attributed to A R Sheth.

At least 145 records · Page 8Linked to original sources
PubMed1981

Presence of inhibin in testes of the Indian water buffalo Bubalus bubalis.

Isolation, purification and characterization of inhibin from the testes of the Indian buffalo Bubalus bubalis is described. The biological activity of the final product, which produced a single band on disc gel electrophoresis, was affected by heat treatment, 6 m urea, pH alteration and pepsin digestion. Chemically, it appeared to be a glycoprotein. The protein and carbohydrate components together accounted for a total of 63% of the dry weight, whereas 37% of the constituents were unknown.

Animals↗
PubMed1981

Evidence for the presence of specific prolactin binding protein in rat seminal vesicle fluid.

The presence of a specific prolactin binding in rat seminal vesicle fluid has been demonstrated. The binding phenomenon was saturable and pH dependent, the optimum pH being 7.2. The prolactin binding protein (PBP, 1800 x g supernatant) was characterized by a single order affinity binding sites with an association constant (Ka) 1.52 x 10(7) mol-1. The specificity of the prolactin binding was demonstrated by the fact that other proteohormones such as rFSH, rLH, rTSH and hTSH failed to inhibit the binding of labelled hormone to PBP, while unlabelled oPRL gave a dose-response inhibition curve. Among the rats in the age group 45 to 180 days, PBP obtained from animals of 90 day old showed a maximum binding of radioiodinated rPRL. Following castration for five days the percent binding of 125I-rPRL to PBP decreased markedly. The treatment of castrated rats with testosterone propionate for five days returned the 125I-rPRL binding to near intact values. Following a centrifugation of 1800 x g supernatant at 360 000 x g for 3 h, prolactin binding activity still remained in the supernatant thus implicating that the binding protein is in a soluble state.

Age Factors↗
PubMed1981

A simple and sensitive color test for the detection of human chorionic gonadotropin.

A simple, rapid, sensitive, and reliable ELISA test for the detection of urinary human chorionic gonadotropin (hCG) has been developed using penicillinase (EC no. 3.5.2.6) as the label and a microtiter ELISA plate as a solid support for antibody. The results of the test is assessed visually for the dark blue that indicates the presence of hCG. A minimum of 500 mU of hCG/ml of urine can be detected easily and the test can be completed in 2 hours with good precision. Accurate negative results are obtained in urine samples of normally ovulatory women, postmenopausal women, and adult males, suggesting that the physiologic concentrations of luteinizing hormone do not influence the assay results. In samples referred for pregnancy detection 95.5% accurate results were obtained when compared with histopathologic and/or clinical findings. There were 2 false-negative results, and only in 4 of 132 subjects studied were false-positive results obtained. This test, if properly performed, should prove a valuable tool in the detection of hCG as early as 30 to 35 days from the last menstrual period.

Chorionic Gonadotropin↗
PubMed1980

Enhancement of serum FSH levels after pinealectomy.

The effect of pinealectomy on the circadian periodicity in serum gonadotropins was investigated in adult male rats. Pinealectomy resulted in an elevation of the serum FSH concentration 7 days after the operation. A 4-fold increase in serum FSH over 24 h with no concommitant increase in serum LH following pinealectomy suggests that the control of FSH secretion could be mediated via the pineal gland.

Animals↗
PubMed1980

Effects of aspirin on blood plasma levels of testosterone, LH and FSH in maturing male rats.

Effects of aspirin administration (5 mg/100 g body weight) for various days were observed on the hormonal levels of maturing male rats. Aspirin was given orally every day for 8, 15, 22 and 30 days. No change in the weights of testes, epididymis, prostate or seminal vesicles was observed after treatment. While aspirin administration for 8 days caused an increase in the plasma testosteone level with decrease in both LH and FSH levels, prolonged treatment for 15 days and more produced a reverse effect, viz. decrease in plasma testosterone and increase in plasma LH and FSH levels. Testicular ascorbic acid content was found to decrease on the 15th and the 22nd day of treatment. Testicular cholesterol was increased after 22nd and 30th days of treatment. Prostatic acid phosphatase activity decreased in all the treated groups. The possible significance of these findings is discussed.

Acid Phosphatase↗