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Biomedical subjects

A Quesnel

Publications and source records attributed to A Quesnel.

30 records · Page 2Linked to original sources
PubMed1987

Increased synthesis of ajmalicine and catharanthine by cell suspension cultures of Catharanthus roseus in response to fungal culture-filtrates.

The ammonium sulfate-precipitated fraction from mycelia and culture-filtrates and the crude, cell-free culture filtrates from the growth medium of the fungi Chrysosporium palmorum, Eurotium rubrum, Micromucor isabellina, and Pythium aphanidermatum when aseptically added to cell suspensions of Cantharanthus roseus caused a rapid and dramatic increase in indole alkaloid biosynthesis. Up to 400 micrograms/L ajmalicine and 600 micrograms/L catharanthine were detected in C. roseus cell suspension grown in the presence of the M. isabellina fungal culture filtrate for 3 d. Untreated cells produced only trace levels of ajmalicine and catharanthine per liter of cell suspension after 15 d of culture.

Cell Line↗
PubMed1985

[Not Available].

Explore the source record for details and available documents.

Demography↗
PubMedDate not supplied

Surgical treatment of lumbosacral instability caused by discospondylitis in four dogs.

OBJECTIVE: To describe a surgical technique involving distraction and stabilization of the lumbo-sacral vertebral segment using an external skeletal fixator in dogs with lumbosacral instability caused by discospondylitis. STUDY DESIGN: Retrospective clinical study. ANIMALS: Four client-owned dogs. METHODS: Medical records of all dogs diagnosed with discospondylitis from 1994 to 1997 were identified and reviewed. Four dogs with lumbosacral discospondylitis requiring surgical treatment were then specifically studied. Surgical technique, clinical signs, preoperative diagnostic investigation, radiographic findings, and the results of short-term and long-term reevaluations were recorded. RESULTS: Twelve dogs with discospondylitis were identified, 4 of which had lumbosacral discospondylitis. These 4 dogs underwent surgical distraction and stabilization because they failed to respond to medical treatment. Three dogs received a cancellous bone graft between L7 and S1 and had rapid interbody fusion of this vertebral segment. The dog that did not receive a graft did not have interbody fusion at the time of fixator removal. This did not affect the final clinical outcome. Lumbosacral pain and neurological deficits present before surgery rapidly subsided after the procedure. All dogs received concurrent antibiotic treatment for a minimum of 4 weeks. All dogs were clinically normal at the time of fixator removal and all continued to do well during the follow-up period (8-48 months; mean, 27.5 months). CONCLUSION AND CLINICAL RELEVANCE: Lumbosacral discospondylitis may not respond well to conservative treatment because of the mobility of the affected space. Surgical treatment involving distraction and stabilization to obtain intervertebral fusion is very effective in treating lumbosacral instability caused by discospondylitis.

Animals↗
PubMedDate not supplied

[Toward a demo-anthropological perspective on nuptiality and its relationship with new reproductive patterns: consideration of a study carried out in the area surrounding Zacatepec, Morelos].

"The research is based on a study carried out between 1989 and 1991 in the three rural communities in the state of Morelos [Mexico] that included a socio-demographic survey and interviews. The methodological goal of the authors is to view nuptiality from demographic and anthropological standpoints.... The authors seek to pinpoint the elements that account for changes that have taken place in nuptiality, as well as to identify the impact of these changes on [descendants]." (SUMMARY IN ENG)

Americas↗
PubMedDate not supplied

[The diversity of reproductive trajectories and institutional interactions].

"This article presents results from research carried out between 1989 and 1991 in a rural region in the state of Morelos [Mexico], beginning from an analysis centered in the interactions between health agents and women.... Using both quantitative and qualitative methodologies, several reproductive trajectories were identified and analyzed, allowing an assessment, on the one hand, of the intensity of women's interactions with health institutions, their community, and their partner and, on the other hand, a characterization of the social trajectories of women as a fundamental factor in their integration with the biomedical system." (SUMMARY IN ENG)

Americas↗
PubMedDate not supplied

Use of synthetic peptide libraries for the H-2Kd binding motif identification.

To identify Kd-binding peptides, an approach based on small peptide libraries has been developed. These peptide libraries correspond to all possible single-amino acid variants of a particular Kd-binding peptide, SYIPSAEYI, an analog of the Plasmodium berghei 252-260 antigenic peptide SYIPSAEKI. In the parent sequence, each position is replaced by all the genetically encoded amino acids (except cysteine). The multiple analog syntheses are performed either by the Divide Couple and Recombine method or by the Single Resin method and generate mixtures containing 19 peptides. The present report deals with the synthesis, the purification, the chemical characterization by amino acid analysis and electrospray mass spectrometry (ES-MS), and the application of such mixtures in binding tests with a soluble, functionally empty, single-chain H-2Kd molecule denoted SC-Kd. For each mixture, bound peptides were eluted and analyzed by sequencing. Since the binding tests were realized in noncompetitive conditions, our results show that a much broader set of peptides bind to Kd than expected from previous studies. This may be of practical importance when looking for low affinity peptides such as tumor peptides capable of eliciting protective immune response.

Amino Acid Sequence↗