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Biomedical subjects

A Pugliese

Publications and source records attributed to A Pugliese.

At least 127 records · Page 7Linked to original sources
PubMed1987

Growth of Candida albicans in a minimal synthetic medium without biotin.

Growth of Candida albicans strain B 311-10 was observed in a minimal synthetic biotin-free medium, using different glucose concentrations, during the first 30 hours of its development at 28 degrees C. The yeast's growth was observed spectrophotometrically at 675 nm reading its optical density every hour. The minimal medium of Shepherd et al., with glucose (15 g/L) and biotin was modified: this vitamin was eliminated and the concentration of glucose was gradually lowered to 0.5 g/L. At 5 g/L of glucose and without biotin very good growth was obtained. Based on our results during the first 30 hours of growth, biotin has no influence on the yeast's growth. This medium would be useful for the study of the physiology of C. albicans during the first period of its development.

Biotin↗
PubMed1986

Effect of betamethasone in lungs of mice treated with lymphocytosis-promoting factor of Bordetella pertussis.

The role of betamethasone in the lungs of mice treated with lymphocytosis-promoting factor (LPF) was studied. Intravenous injection with 10 ng of LPF produced significant lymphocytosis and monocytosis five days later. Two groups were also treated with betamethasone: the first with daily administrations and the second at zero-time with LPF. Lymphocytosis was not as high as in the group treated only with LPF (P less than .05), and lymphocytosis was lower in the second group than in the first (P less than .05). In lungs of mice treated with LPF, lymphocytes accumulated relative to lymphoid organs such as the spleen, where a marked depletion was noted. Administration of betamethasone either with LPF or after one day did not cause accumulation of lymphocytes in lungs, whereas increased spleen cellularity was observed. These data were also supported by histological changes, i.e., diminished congestion and edema of the lung and reduction of mononuclear cells in parenchyma.

Animals↗
PubMed1986

In vitro activity of alpha, beta, gamma human interferons and Aspergillus terreus mycotoxins on cell proliferation.

The in vitro effect of different doses of alpha, beta and gamma human interferons and their interaction on the proliferation rate of human erythroleukemic cell line (K-562) and other cell lines has been evaluated. The results show that the stronger activity is exerted by beta HIF in comparison with alpha HIF. The action is selective and species-specific. beta HIF never elicited an "enhancing" effect on cell proliferation; on the contrary this phenomenon has been observed to occur with alpha and gamma HIF. In our cell system, the interaction between beta and gamma HIF gave a synergistic effect; on the contrary, no interaction has been observed to take place when beta HIF and directly cytotoxic mycotoxins extracted from A. terreus are associated.

Cell Division↗
PubMed1986

Thymostimulin therapy in patients with measles meningoencephalitis.

Four children with measles meningoencephalitis were treated with thymostimulin, a calf thymic extract. All patients were comatose when therapy was started. In all but one a good and sometimes rapid clinical improvement was observed. In the responsive patients no neurological or EEG sequellae were noted over a follow-up period of 1-4 years. The depression of cell-mediated immunity, present in some patients before treatment, normalised at the end of therapy.

Child↗
PubMed1985

Defective interferon-alpha production in children with recurrent respiratory tract infections. A primary or secondary deficiency?

Interferon-alpha and -gamma production was assayed in 44 children with recurrent respiratory tract infections, in 9 patients with recurrent stomatitis and in 32 control subjects. A partial, transitory deficiency in interferon-alpha production was found in 2 children with recurrent respiratory tract infections. The deficiency may be either cause or effect of the recurrent infections. Many data support the latter hypothesis.

Adolescent↗
PubMed1984

Evidence of circulating interferon-gamma in newly diagnosed diabetic children.

Acid-stable interferons (IFN-alpha or IFN-beta) are produced by nucleated cells infected by virus, while acid-labile interferon (IFN-gamma) is synthesized by activated T-lymphocytes. IFN-gamma or an atypical form of acid-labile IFN-alpha have been observed in the circulation of some patients with autoimmune disease. It is believed that autoimmunity and/or viral infections are involved in the pathogenesis of insulin dependent diabetes mellitus. We therefore examined the sera of newly diagnosed diabetic children for the presence of virus-induced or acid-labile IFN. Significant IFN levels (greater than or equal to 8 U/ml) were observed in 11 of 29 patients when compared to 31 healthy children in the same age range. The inhibitor is characterized by species specificity, acid-lability and neutralization with anti-serum for IFN-gamma.

Antiviral Agents↗
PubMed1983

Adhesion of Proteus species to various cell types.

Pathogenic and saprophytic Proteus strains from the urine of patients with urinary tract infections and healthy adults respectively were evaluated with regard to their ability to adhere in vitro to homologous cells (uroepithelial cells from urinary sediment and cultured WISH cells) and heterologous cells (RK-13 and MDCK cells). The effect on attachment of pretreating bacteria with subinhibitory concentrations of piperacillin and sagamicin was also determined for one sensitive and one resistant strain. Fifty percent of the pathogenic Proteus strains demonstrated good adherence to human urinary epithelial cells, whereas saprophytic strains did not adhere. There was a lower rate of attachment to culture cells. Piperacillin and sagamicin in subinhibitory concentrations caused a decrease in the attachment of the sensitive Proteus strain.

Adhesiveness↗
PubMed1980

[Viral respiratory infections].

A brief account of the general features of viruses and their importance in the biological world is followed by a description of the main viruses responsible for respiratory infections in man, with particular reference to matters of morphology, epidemiology, pathology, and (where possible) prophylaxis. Lastly, the modalities for the isolation of such viruses and the main serological reactions employed in diagnosis are explained.

Adenoviridae Infections↗
PubMed1980

[Hemagglutination inhibition test for rubella virus: control of the removal of complete non-specific inhibitors].

A method showing whether the inhibitory activity of a serum, with a low rubella HAI titer, is due to specific antibodies, or to the incomplete removal of non-specific beta-lipoprotein inhibitors, is described. Serum absorption with Staphylococcus aureus removes over 95% of the IgG, and a smaller amount of the other Ig, beta-lipoproteins and albumin, whereas Staphylococcus epidermidis absorbs about 20% of all Ig, beta-lipoproteins and albumin. This means that a serum with anti-rubella IgG displays the same, or at most a slightly depressed HAI titer after absorption with S. epidermidis, whereas S. aureus leads to at least a 16 fold reduction. By contrast, a serum with no antibodies, in which inhibition is due to non-specific inhibitors, will show an equally reduced HAI titer after absorption with each strain.

Absorption↗
PubMed1980

Interferon production on mouse fibroblasts induced by various viruses.

The interferon (IF) inducing ability of some DNA and RNA viruses on mouse CCL-1 cells was investigated. The F and H strains of Newcastle disease virus (NDV) and Sendai virus proved to be the best inducers. GD-7, Mengo and Vesicular stomatitis virus (VSV) were middle inducers, Herpes simplex 1 (HSV 1) was a weak inducer, and Adenovirus 12 and Semliki forest (SFV) were non-inducers. Moreover antiviral resistance induced by the same viruses and IF production generally followed parallel paths.

Animals↗
PubMed1980

Polygenic control of interferon production induced by poly I:C in vivo. Brief report.

Poly I:C-induced interferon production shows quantitative differences in various mouse strains depending on multiple interactions among genes located both inside and outside the major histocompatibility complex. Moreover, circulating interferon levels do not appear to be under direct genetic control but, rather, to depend on the gene-coded intensity of specific recognition of the inducer.

Animals↗
PubMed1979

Effect of synthetic polynucleotides on the growth of transplantable tumours in BALB/c mice.

The effect of BALB/c mice pretreatment with tumour cells (a mammary adenocarcinoma, ADK-1t and an IgA secreting plasmocytoma, MOPC-315) adsorbed with poly I:C, poly I and poly C is examined. Only mice pretreated with cells of both tumours adsorbed with poly I:C and poly C proved to be extensively protected against challenge by homologous untreated tumour cells, whereas this was not so in the case of poly I. A possible explanation of this phenomenon is discussed.

Animals↗