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Biomedical subjects

A Poole

Publications and source records attributed to A Poole.

81 records · Page 5Linked to original sources
PubMedDate not supplied

In vitro correlates of mineral dust toxicity.

In vitro studies of mineral fibres have shown that these materials are capable of causing the production of free radical damage (measured by the accumulation of thiobarbituric acid-reactive material) in a way that does not depend on fibre morphology but rather on a surface property, probably the presence of iron. The DNA-damaging activities of the same dusts were measured in an assay based on the production of single-stranded DNA. Activity in this assay was again not dependent on the presence of fibres. In contrast the cytotoxicity of mineral fibres towards V79-4 cells correlated better with in vivo tumorigenicity than did either of the above activities. The activity of fibres against a macrophage-like cell demonstrated that both fibre morphology and chemical properties are important determinants of biological activity.

Asbestos↗
PubMedDate not supplied

Modification of the in vitro activities of amosite asbestos by surface derivatization.

The in vitro cytotoxicity of amosite asbestos and its silanized and bovine serum albumin conjugated derivatives were studied in two cell lines. The overall cytotoxicity of amosite towards Chinese hamster lung cells (V79-4) was not affected by derivatization, although the rate of cell-particle interaction was reduced. Protein derivatization virtually abolished the cytolytic action of amosite to the macrophage-like cell line (P388D1), although this derivatized material still induced the release of arachidonate. These results confirm that the in vitro cytotoxic effect of fibrous particulates toward V79-4 cells is mainly dependent on fiber size, while the response of macrophage-like cells is more sensitive to alterations in surface chemistry. The relationship between these results and the pathogenicity of asbestos is discussed.

Animals↗