Search PubMed⌕ Search

Biomedical subjects

A Pilloni

Publications and source records attributed to A Pilloni.

6 recordsLinked to original sources
PubMed1998

The effect of hyaluronan on mouse intramembranous osteogenesis in vitro.

Hyaluronan (HA) is an almost ubiquitous component of extracellular matrices. Early in embryogenesis mesenchymal cells migrate, proliferate and differentiate, in part, because of the influence of HA. Because many of the features of embryogenesis are revisited during wound repair, including bone fracture repair, this study was initiated to evaluate whether HA has an effect on calcification and bone formation in an in vitro system of osteogenesis. Enzyme-digested calvarial mesenchymal cells from 13-day-old mouse embryos were cultured in BGJb medium with rooster comb hyaluronan in seven different molecular weights (30, 40, 90, 160, 550, 660, and 1300 kDa). The dosages for each molecular weight were 0.5, 1.0 and 2. 0 mg/ml. HA was added once to the medium at the plating of cells. After 10 days in culture, with low molecular weight hyaluronan (30 and 40 kDa) bone colonies were identifiable on a base of confluent fibroblasts. The number of colonies was larger than controls, particularly in the 1.0 and 2.0 mg/ml dosages of both 30 and 40 kDa of HA. Hyaluronan of high molecular weight, no matter what the dose, showed no significant bone colony formation, with apparent cell growth inhibition. Higher molecular weights were thereafter not included in this study. No statistically significant difference in the size of colonies was found when compared to controls in the 30 and 40 kDa bone colonies no matter what the dose.

Adjuvants, Immunologic↗
PubMed1998

Surgical treatment of cyclosporine A- and nifedipine-induced gingival enlargement: gingivectomy versus periodontal flap.

The purpose of this study was to compare probing depth resolution achieved by gingivectomy and periodontal flap techniques in the treatment of cyclosporine A- and nifedipine-induced gingival enlargement. Ten kidney transplant patients who were receiving cyclosporine A and nifedipine for at least 6 months participated in the study. Five patients were randomly assigned to the gingivectomy group and 5 patients to the periodontal flap group. Only anterior segments of the oral cavity (canine to canine) were surgically treated. Clinical measurements, including probing depths, plaque index, and gingival sulcus index, were taken at baseline, 6 weeks, 6 months, and 1 year. Results showed that probing depths, while similar for both groups in the first 6 weeks of the study, were significantly shallower for the periodontal flap group when compared to the gingivectomy group at 6 months (2.48 +/- 0.34 mm versus 4.87 +/- 0.79 mm, respectively) and 1 year (322 +/- 0.65 mm versus 6.40 +/- 1.02 mm, respectively). Within its limitations, this study suggests that the pocket reduction achieved by the periodontal flap may be sustained for longer periods of time than by the gingivectomy technique in the treatment of cyclosporine A- and nifedipine-induced gingival enlargement.

Adolescent↗
PubMed1997

Assessment of the acrylic resin Technovit 7200 VLC for studying the gingival mucosa by light and electron microscopy.

Technovit 7200 VLC is an acrylic resin formulated for embedding undecalcified hard tissues which are prepared for light microscopy according to a cutting-grinding technique. To employ this resin for embedding and cutting soft tissues by ultramicrotomy, we carried out a qualitative study on biopsies of canine gingival mucosa using light and transmission electron microscopy. For a critical evaluation of this resin, some biopsies were embedded in Agar 100, an epoxy resin widely used in morphological studies. At the light microscopic level the samples embedded in Technovit 7200 VLC showed good morphology and excellent toluidine blue staining of different cell types and extra cellular matrix. At the ultrastructural level, nuclei, cytoplasmic organelles, collagen fibrils and ground substance appeared well preserved and showed high electron density. The acrylic resin was stable under the electron beam and its degree of shrinkage appeared to be very low. We conclude that Technovit 7200 VLC can be employed for ultramicrotomy for both light and electron microscopic investigation of soft tissues.

Acrylic Resins↗
PubMed1990

[Impacted maxillary third molars. Accidental dislocation].

During surgical removal of impacted maxillary third molars, many complications may occur. Their accidental displacements in the maxillary sinus or in the infratemporal fossa are not too rare in the common general dentistry. Presenting this case, the Authors want to demonstrate that, when each one of this kind of teeth are well studied before their treatment, many complications can be avoided and, the sooner the dentist is able to solve them when they occur, the better the post-operative course will be.

Adult↗