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Biomedical subjects

A Perino

Publications and source records attributed to A Perino.

69 records · Page 4Linked to original sources
PubMedDate not supplied

In vitro sperm capacitation and intrauterine insemination (IVC-Insem): a simple technique for the treatment of refractory infertility unrelated to female organic pelvic disease. Clinical results and immunological effects: a preliminary report.

12 couples, with infertility due to male subfertility, female antisperm isoimmunization, male antisperm autoimmunization, cervical mucus insufficiency or unexplained infertility underwent intrauterine A.I.H. with capacitated sperm and controlled ovarian stimulation. The pregnancy percentage was of 41.6% per woman treated and of 31.2% per treatment cycle; five pregnancies were obtained, of which two resulted in abortions. Five cases of oligoasthenospermia were treated, three severe and two moderate; two pregnancies occurred in the former and one in the latter. S.I.T. and S.I.T.-cap. were performed on the serum, and Micro-S.I.T. and Micro-S.I.T.-cap. on the cervical mucus in order to exclude the possibility that intrauterine A.I.H. with capacitated sperm might have caused capacitated or non-capacitated antisperm immunization. Intrauterine A.I.H. would seem to be a useful alternative to G.I.F.T. and to I.V.F./E.T. or at least a technique which should be performed before these two in cases of infertility not caused by female organic pelvic disease; it is cheap, simple and extremely well-accepted by the patient.

Adult↗
PubMedDate not supplied

An ELISA for antisperm antibody detection in serum: comparison with TAT and SIT in serum, with MAR-test, immunobead-test and TAT in semen and with micro-SIT in cervical mucus.

16 couples belonging to couples with negative or doubtful PCT were selected according to the presence of antisperm immunization. 12 patients, 5 male and 7 female, showed both localized and generalized immunization. The former was diagnosed by means of a positive IgG MAR-Test, direct IgG Immunobead-Test, direct IgG Immunobead-Test and seminal TAT in the male patients, and Micro-SIT in the cervical mucus of the female patients, while for the latter there was simultaneous positivity of both serum TAT and SIT, except for two cases, in which the SIT only was positive. The 4 remaining patients, 2 male and 2 female, did not show any signs of antisperm immunization. The evaluation of the antisperm antibodies by means of the ZER ELISA Antisperm Kit in the serum of the 16 patients examined showed that there were no significant statistical differences between the serum TAT and the SIT. The former showed agreement of the results in 93.75% of the cases, and the latter in 81.25%. A strict correlation was observed between the ELISA for serum antisperm antibodies (ELISA-AS-Abs) and the local immunitary situation, with agreement in 93.75% of the cases. The ELISA-AS-Abs seems to bring the advantage of eliminating the need for fresh semen for antibody titration and also means that there is no subjective interference with the evaluation of the results.

Adult↗
PubMedDate not supplied

Effects of in-vitro capacitation of human spermatozoa on the action of the sperm-immobilizing and sperm-agglutinating antibodies. Possible therapeutic applications on female antisperm isoimmunization.

In 51 serum samples (31 originating from infertile women, 20 from infertile men) with significant sperm immobilizing activity (S.I.T. with S.I.V. greater than 2) we carried out the following tests using semen from three proven fertile donors with excellent semenological characteristics: repetition of S.I.T.; carrying out of S.I.T. with sperm previously subjected to in vitro capacitation (S.I.T.-cap.); carrying out of T.A.T.; carrying out T.A.T. with sperm previously subjected to in-vitro capacitation (T.A.T.-cap.). Nor was there statistically significant difference evident in the results of the tests in relation to the use of the semen of the three different donors. In all 51 samples the repetition of S.I.T. involved the confirmation of sperm-immobilizing activity, while the result of such activity was constantly negative when it was carried out with S.I.T.-cap. In female subjects the comparison of the results of S.I.T. and S.I.T.-cap. showed a statistically significant difference (p less than 0.001). 38 out of the 51 serum samples examined showed positive T.A.T. with variant titers from 1:32 to 1:512 and with different types of agglutination (H, T, tip, M). In all the serum examined, T.A.T.-cap. resulted positive with a titer of 1:512 and agglutination of type H. The results of S.I.T.-cap. seem to underline the advantages linked to the use of capacitated semen in A.I.H. and in G.I.F.T. The evaluation of the results of T.A.T.-cap. make us suspect that they are not linked with the presence of antibodies in tested sera.

Agglutination Tests↗
PubMedDate not supplied

Diagnosis of cervical lesions from human papova virus.

The impact of human papillomavirus (HPV) infection in genital pathology has been clarified relatively recently and its relevance appears to be parallel to the liberalization of sexual customs. Aside from some aspects of the infection grossly evident to the naked eye, an increasing number of sub-clinical lesions, that can be colposcopically identified as "flat condylomata" is commonly revealed by cytologic screenings. The Authors report their experience in the diagnosis of this frequent pathology in gynecologic practice, underlining the importance of more fine laboratory investigations, mostly ultrastructural and immunohistochemical.

Cervix Uteri↗
PubMedDate not supplied

Hysteroscopic treatment of uterine septa.

Patients with septate uterus usually undergo reproductive failures with recurrent abortions and premature deliveries. Up till now surgical therapy for such a defect has relied on different abdominal metroplasty procedures reflecting the need to establish normal anatomy and physiology of the uterus. Nowadays, wide experience accumulated in hysteroscopic management of intrauterine adhesions, has allowed the incision of the uterine septa with the hysteroscopic technique. In this report the Authors describe the surgical technique of the hysteroscopic metroplasty in eleven cases. Initial results are encouraging: surgical outcome is good, with no intra- or postoperative morbidity; if the pregnancy outcome rate confirms these results, then the traditional abdominal surgical approach should become an out-moded therapy, so making hysteroscopic metroplasty the treatment of choice for the uterine septa.

Abortion, Spontaneous↗
PubMedDate not supplied

Hysteroscopic reversible tubal sterilization.

The Authors describe their experience with a new type of intratubal mechanical device to be inserted under hysteroscopic control. Up to present time, 1471 cycles without any other form of contraception have been observed. Although the promising results of this preliminary report in the field of hysteroscopic reversible tubal sterilization, the Authors underline that this new technique is still evolving and will be improved with further experience.

Contraceptive Devices, Female↗
PubMedDate not supplied

Diagnosis and management of intrauterine adhesions by microhysteroscopy.

The Authors report their experience in the diagnosis and management of intrauterine adhesions in a group of 23 patients using the microhysteroscope. This new model of hysteroscope makes it possible to diagnose clinically suspected cases and to perform lysis of the adhesions in the out-patient department, without cervical dilatation and anesthesia. The technical features of the microhysteroscope made a double evaluation of adhesions possible: a) the macroscopic classification of the site and extension of the synechiae and b) their microscopic in vivo observation to identify the less vascularized areas and to perform adhesiolysis under direct visual control. Monthly hysteroscopic examinations were performed in order to follow the results of therapy and to remove residual or newly formed adhesions. In the authors' experience the microhysteroscope may be successfully used in the early diagnosis and subsequent best management of intrauterine adhesions in patients with previous medical history of curettage in a recent pregnant uterus.

Adolescent↗
PubMedDate not supplied

[Endocrinological problems in male adolescents].

The male adolescent may present several endocrinological problems, the most frequent of which is the retardation or absence of puberty due to constitutional delay of growth and development. This form does not require therapy and must be distinguished from other forms of hypogonadism (primitive or secondary) by endocrine tests (LHRH test, nightly pulses LH secretion, plasmatic basal level of testosterone and after HCG, cerebral NMR). Hypogonadism treatment consists of replacement therapy with testosterone or testes stimulation with HCG or LHRH. Another frequent disease is gynecomastia, usually due to physiological enlargement of mammary gland during pubertal development, sometimes it may be secondary to hypogonadism, tumors, liver function abnormalities. Severe or psychologically disturbing gynecomastia can be corrected by reductive mammoplasty. Very often, adolescents may present diseases related to incorrect food habits. Obesity is common and anorexia is becoming an important problem also in males.

Adolescent↗
PubMedDate not supplied

[Endocrinologic problems of the male adolescent].

The male adolescent may present several endocrinological problems, the most frequent of which is the retardation or absence of puberty due to constitutional delay of growth and development. This form does not require therapy and must be distinguished from other forms of hypogonadism (primitive or secondary) by endocrine tests (LHRH test, nightly pulses LH secretion, plasmatic basal level of testosterone and after HCG, cerebral NMR). Hypogonadism treatment consists of replacement therapy with testosterone or testes stimulation with HCG or LHRH. Another frequent disease is gynecomastia, usually due to physiological enlargement of mammary gland during pubertal development, sometimes it may be secondary to hypogonadism, tumors, liver function abnormalities. Severe or psychologically disturbing gynecomastia can be corrected by reductive mammoplasty. Very often, adolescents may present diseases related to incorrect food habits. Obesity is common and anorexia is becoming an important problem also in males.

Adolescent↗