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Biomedical subjects

A Pasternack

Publications and source records attributed to A Pasternack.

At least 217 records · Page 12Linked to original sources

Effect of furosemide on the urinary excretion of some plasma proteins.

In 30 patients with a variety of glomerular renal disorders, intravenously administered furosemide was associated with a significant increase of the urinary excretion of the protein fraction with a molecular weight higher than 50,000. The excretion of protein with a molecular weight less than 50,000 was not altered. In relation to endogenous clearance of creatinine the clearance of transferrin, IgG and alpha2-macroglobulin increased significantly. In relation to transferrin clearance, the clearance values of albumin and IgG remained unchanged, whereas that of alpha2-macroglobulin increased significantly. These results support the view that furosemide increases the permeability of the glomerular capillary for serum proteins.

Adolescent↗

Evidence of both anti-GBM and immune complex mediated pathogenesis in the initial phase of Goodpasture's syndrome.

A 19 year old women presented with pulmonary hemorrhage, rapidly progressive glomerulonephritis and antiglomerular basement membrane antibody in her serum. In the initial phase of the disease a double layered linear deposit of IgG was seen on the glomerular capillary basement membrane together with a finely granular or focal and lumpy single layer deposit of C3 and numerous small subepithelial electron dense deposits. These findings are suggestive of the simultaneous occurrence of anti-GBM and immune complex glomerulonephritis.

Adult↗

Clearance ratios of amylase and isoamylase to creatinine in renal disease.

Amylase to creatinine clearance ratios were measured in 66 patients with a variety of moderate and severe renal diseases including 10 patients with renal transplants, and in 13 healthy controls. Only in patients with severe renal insufficiency (serum creatinine level above 660 micronmoles/1) were the amylase to creatinine ratios significantly raised. The ratios correlated neither with the type of renal disease, i.e. glomerular or tubulointerstitial, nor with the degree of proteinuria. Patients with renal transplants did not differ from other patients. Clearance ratios of pancreatic and salivary isoamylase to creatinine changed in parallel to that of total amylase. The results suggest that in severe renal failure the loss of nephrons results in decreased fractional reabsorption of amylase in the tubules.

Amylases↗

Aspiration biopsy of the kidney with a new fine needle: a way to obtain glomeruli for morphological study.

An improved fine needle is described for aspiration of kidney glomeruli. The needle, with a sharp inner edge 0.43 mm in diameter, is less traumatic than ordinary percutaneous kidney biopsy needles. Electron microscopy of specimens aspirated from kidneys of normal rabbits showed well-preserved glomeruli. Twenty-two of twenty-six specimens obtained from human kidneys by fine-needle aspiration contained glomeruli. Electron microscopic study of specimens aspirated from 12 patients showed that the needle provides adequate tissue for the diagnosis of diffuse glomerular diseases. The use of the fine needle widens the scope for studying the relation between glomerular fine structure and renal symptoms in many conditions in which conventional methods would not be justified.

Animals↗

Tissue antibodies in malignant and benign urogenital disease.

The presence of anti-tissue antibodies in sera from patients with genitourinary carcinomas and non-malignant genitourinary diseases was studied by indirect immunofluorescence. An increased incidence of various tissue antibodies was observed in carcinomas of the urinary bladder, prostate and kidney when compared to controls. There was a trend towards association between antinuclear antibodies and metastatic undifferentiated tumors. A low degree of malignancy was associated with antibodies against connective tissue "reticulin". A striking similarity between tissue antibody "patterns" in carcinoma patients and patients with chronic renal inflammation was seen. This points to inflammation as being an important factor in tissue antibody production.

Adult↗

The renal lesion in congenital chloride diarrhea.

Congenital chloride diarrhea is an inherited defect of active intestinal Cl- transport which results in a large wastage of electrolytes and water. The effects of this disease and of replacement therapy on renal histology, function, growth, and the renin-angiotensin-aldosterone system were studied in 18 patients. When the patients were given KCl supplement, histologic findings included juxtaglomerular hyperplasia hyalinized glomeruli, calcifications, and arteriolar changes. Renal function and growth were reduced, and the hormonal activities were high. These abnormalities were evidently due to chronic dehydration. The dehydration could be corrected by increasing the dose of KCl, but only the addition of NaCl corrected the hyperaldosteronism. Adequate replacement therapy prevented the renal involvement.

Child↗

HLA-B27 in rheumatoid arthritis and amyloidosis.

To study the role of genetically determined immune responsiveness in the pathogenesis of systemic amyloidosis complicating rheumatoid arthritis the HLA antigens were identified in 26 patients with rheumatoid arthritis complicated by secondary amyloidosis, in 44 patients with rheumatoid arthritis, and in 11 patients with secondary amyloidosis of non-rheumatoid origin. Subjects with ankylosing spondylitis, sacroiliitis without peripheral polyarthritis, Reiter's disease, reactive arthritis, erosive osteoarthritis, psoriatic arthropathy, systemic lupus erythematosus or arthritis associated with a gastrointestinal involvement were excluded from the study. Patients with amyloidosis secondary to rheumatoid arthritis had a high frequency of the HLA specificity B27 and of the haplotype likely to bear A2, B27. The association with B27 was closest in the group of male patients with amyloidosis whose rheumatoid arthritis had begun at an early age and who lacked demonstrable rheumatoid factor in serum. These patients may represent a genetically determined subentity of rheumatoid arthritis.

Adolescent↗

Clinical application of renal aspiration biopsy with a modified fine needle.

Electron microscopic study of solitary glomeruli obtained by aspiration with a modified fine needle is of clinical value in the diagnosis of various renal diseases. The small size of the needle makes it less traumatic than an ordinary percutaneous biopsy needle and therefore suitable for use in situations where a conventional biopsy is for some reason considered undesirable.

Basement Membrane↗

Low serum bilirubin in chronic renal failure. Relation to haem metabolism.

In renal failure serum bilirubin values are lower than normal. When renal failure is moderate this decrease is not due to a reduced-red-cell mass or to low levels of serum albumin. In splenic fine-needle aspirates from patients with renal failure, conditions for haem degradation were normal as indicated by normal activities of haem oxygenase and biliverdin reductase. Nor were the in vitro activities of these enzymes significantly depressed in the presence of ultrafiltrates of uraemic sera or of high concentrations of creatinine, urea or methylguanidine. The capacity of plasma from patients with renal failure to bind bilirubin was less than that of normal plasma, which at least partially explains the observed low values of serum bilirubin. There remains the possibility that in renal failure some bilirubin is removed from the plasma by a hitherto unknown route.

Bilirubin↗

Psychological and social problems encountered in active treatment of chronic uraemia. II. The living donor.

Sixty-four kidney donors have been interviewed by a psychiatrist and given the Rorschach test 6 months-6 years after the transplantation. Twenty-three of these donors were also interviewed before the operation. In addition, each case was studied in detail at a case conference. On the basis of the data thus obtained, an analysis was made of the central dynamics of the donors' personalities, of how they had experienced the donation, and of how they had adapted to it. No psychic trauma was observed in 20 of the subjects, and in 5 of these the operation had apparently had a beneficial effect on their psychic well-being. That no trauma was observed in 8 additional donors was due largely to the effects of a multitude of other traumatic life experiences. Mild trauma had been experienced by 24 donors and moderate to severe by 12. In our study, the donation of a kidney to a sibling turned out to be more traumatic than the donation to a child, and a transplantation with an unsuccessful outcome was more often associated with psychic trauma to the donor than was a donation with a successful outcome. Psycho-social factors that substantially lessened the likelihood of trauma were: good inner resources, flexible defence mechanisms, good mental health and mild compulsive traits. Factors that favoured traumatization were: poor living conditions, interpersonal problems, limited inner resources, low self-esteem (narcissistic problems) and severe psychic deviancy. The results clearly show 1) that potential donors ought to be given adequate time not only to consider their decision but also to prepare themselves for the actural donation, and 2) that supportive help should be offered, both before and after transplantation, to donors whose psycho-social profiles reveal them to be vulnerable to traumatization.

Child↗

Kidney function and compensatory growth of the kidney in living kidney donors.

Pre- and postoperative kidney size and kidney function were studied in 46 living kidney donors aged 20-74 years. Kidney size was measured by planimetry and by estimation of a renal index. Kidney function was assessed by endogenous creatinine clearance and serum creatinine. Planimetry was superior to the renal index for expressing changes in renal size. Compensatory renal hypertrophy took place in donors up to the age of 74, but the greatest changes in renal size were observed in donors of under 40. Total renal function decreased postoperatively to about 77% of the initial level; this change in renal function was inversely correlated with age, but in all subjects studied the function remained within normal limits.

Adult↗

Plasma, red cell and cerebrospinal fluid concentrations of myoinositol in patients with severe chronic renal failure.

A study was made of 24 patients with severe chronic renal failure; 14 of them were undergoing regular haemodialysis treatment 3 times weekly. Plasma, red-cell and cerebrospinal fluid concentrations of myoinositol were increased in all the patients treated conservatively. The plasma level of myoinositol correlated with the plasma level of creatinine (r = 0.78). The plasma myoinositol level increased more than the CSF and red-cell levels, indicating that the myoinositol in the red cells and the CSF originated mostly in plasma. Dialysis for eight hours produced a fall of about 50% in the level of myoinositol in plasma while the decrease in red-cell myoinositol was negligible. This lead to an osmotic grandient between extra- and intra-cellular myoinositol which was however small in molar terms and did not correlate with symptoms of central neurological disturbances.

Creatinine↗

Plasma, red cell and cerebrospinal fluid concentrations of mannitol and sorbital in patients with severe chronic renal failure.

The concentrations of mannitol and sorbitol in plasma, red cells, cerebrospinal fluid and urine were determined in 24 patients with chronic renal failure; 10 of them were on conservative treatment and 14 were haemodialysed three times weekly. The mannitol concentration was significantly increased in the plasma and the cerebrospinal fluid of the uraemic patients compared with the controls. In six out of ten uraemic patients mannitol clearance values exceeded creatinine clearance values. The plasma concentration of sorbitol was undetectable or very low in all patients and control subjects. Red-cell and cerebrospinal fluid concentrations of sorbitol showed a large individual variation in the uraemic patients on conservative treatment and did not correlate with kidney function. During dialysis the mannitol concentration decreased, leading to a small osmotic gradient between the plasma and the red cells. The changes in the concentration of mannitol during dialysis showed no connection with the symptoms of central nervous disturbance which appeared during dialysis treatment. The red cell sorbitol concentration during dialysis increased by about 20%. There was a correlation (p less than 0.05) between t,e increase in sorbital in cereemic patients.

Creatinine↗