Biomedical subjects

A Park

Publications and source records attributed to A Park.

110 records · Page 7Linked to original sources

PubMedDate not supplied

Diagnosis of intestinal ischemia in the rat using magnetic resonance imaging.

Noninvasive diagnosis of persistent ischemia after intestinal revascularization has remained an elusive goal. Because magnetic resonance imaging (MRI) can detect changes in tissue water, we studied its efficacy in differentiating ischemic from perfused intestine in an animal model. Six-week-old rats were subjected to (1) 30-min superior mesenteric artery (SMA) occlusion and reperfusion, (2) permanent SMA ligation, or (3) sham operation, and were then imaged for 90 min using a small-animal MRI scanner with T1 weighting (TR = 1000 msec, TE = 25 msec). In an additional group of rats, the experiment was repeated using a new contrast technique consisting of oral ferrite to decrease luminal signal and intravenous gadolinium to increase bowel wall signal. Mean abdominal intensity over the scanning period was calculated for each animal (n = 5 rats per experimental group). Definition of individual bowel loops was subjectively improved in animals scanned with intravenous and oral contrast. Mean abdominal intensity was significantly lower in ligated vs sham rats (43.90 +/- 8 vs 59.63 +/- 6 and 46.19 +/- 6 vs 54.26 +/- 6, with and without contrast, respectively). There was no significant difference in intensity between reperfused and sham animals. MRI differentiated persistently ischemic bowel from viable bowel in this model, both with and without the use of contrast. These data suggest that MRI may have a potential role in the noninvasive diagnosis of persistent intestinal ischemia.

Administration, Oral↗

PubMedDate not supplied

Diagnosis of persistent intestinal ischemia in the rabbit using proton magnetic resonance imaging.

Noninvasive diagnosis of persistent intestinal ischemia remains an elusive goal. Magnetic resonance imaging (MRI) recognizes changes in tissue water content, and several authors have demonstrated increased intensity within 6 hours of intestinal ischemia. To simulate the clinical situation more closely, we studied the efficacy of MRI in differentiating ischemic from viable segments of bowel 24 hours after injury in a rabbit model. A segment of distal ileum was rendered ischemic by vascular isolation and ligation. Controls underwent sham operation without vascular ligation. After 24 hours, multislice transverse scans were done using both T1 and T2 weighting. Image intensity was calculated from the isolated loop (absolute intensity), and paraspinal muscle intensity was used as an internal standard to calculate relative intensity (isolated bowel/paraspinal muscle). Animals were killed and bowel necrosis was confirmed histologically. Both absolute and relative intensity were significantly higher in animals undergoing persistent intestinal ischemia. This was true using both T1 and T2 weighting. In a further group of rabbits using the same model, intensity was calculated both before and after intravenous gadolinium. No significant difference was seen between sham and ischemic animals. Our data show that (1) MRI can differentiate ischemic from viable bowel 24 hours after ischemic injury, and (2) the use of intravenous contrast does not improve accuracy. We conclude that MRI may represent a useful noninvasive technique for the diagnosis of persistent intestinal ischemia and that clinical studies should be initiated.

Animals↗