Biomedical subjects

A P Sadkov

Publications and source records attributed to A P Sadkov.

At least 19 recordsLinked to original sources

PubMed2002

Gold helps bacteria to oxidize methane.

With the use of labeled methane-14C and by chromatographic analysis it was shown that gold-containing protein ("Au-protein"), isolated from goldphilic Micrococcus luteus bacteria, catalyzes the oxidation of methane to methanol in the system also containing NADH, air, K(3)Fe(CN)(6) and Tris-HCl buffer. Presumably Au-protein helps bacteria to survive when usual sources of carbon and energy are scarce.

Gold↗

PubMed1997

Participation of biological membranes in colloidal gold transformation by Micrococcus luteus cells.

Methods of biochemical and physicochemical analysis were applied for studying the role of Micrococcus luteus cell structure in gold accumulation. It was shown that membrane proteins were the main factors in this process. Moreover, different quinones played the main role in the redox transformation of gold.

Absorption↗

PubMed1984

[Localization of the FeMo-cofactor in the active center of nitrogenase].

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Binding Sites↗

PubMed1980

Spectroscopic investigation of FeMo-cofactor. Coenzyme A as one of the probable components of an active site of nitrogenase.

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Azotobacter↗

PubMed1977

[Study of the topography of the nitrogenase active center by the electron microscopy method with the use of the electron density labels].

The electron microscopy method has been used for studying the topography of the active centre of the nitrogenase in combination with the method of electron density labels. The active centre has been shown to place on the nitrogenase macromolecule asymmetrically. The non-heme iron atoms of the nitrogenase active centre have been regularly packed and located on the macromolecule as a quadrangular cluster closely to the surface of subunits. Two free sulfhydryl groups of the ATP-site of the nitrogenase active centre are in close proximity to the cluster. ATP-site of the nitrogenase active centre containing these groups is located on the Fe-enzyme, while the main part of the iron-containing cluster is located on the Mo-Fe-enzyme.

Adenosine Triphosphate↗

PubMed1976

[Isotopic effect in enzymatic oxidation of methane].

The isotopic effect during oxidation of methane and deuteromethane by a suspension of Methylomonas rubrum cells, for which methane is the only source of carbon, was observed. The rate of CH4 oxidation is 12.5 times higher than that of CH4 oxidation. It is demonstrated that CD4 is a competitive inhibitor of CH4 oxidation. The results obtained suggest that the disruption of the C-H bond is the limiting step of enzymatic oxidation of methane.

Deuterium↗

PubMed1973