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Biomedical subjects

A P Ponomarev

Publications and source records attributed to A P Ponomarev.

16 recordsLinked to original sources
PubMed1992

[The electrophysiological properties of the structural polypeptides of the foot-and-mouth-disease virus].

The methodological approaches of isolation of preparations of FMDV structural polypeptides to analyse them by the electrophoresis and electro-focussing methods are presented. The value of isoelectric points of protein coat of FMDV structural polypeptides and corresponding them values of electric potential are determined. The similarity and differences of FMDV serotypes, characterized by the value of relative surface, falling on separate polypeptides, are determined for the virion structure on the basis of superposition principle. FMDV has been shown to possess the summarized negative charge of different values. The charge depends on the virus type and it is a determining condition for viruses resistance in environment. A graphical model of FMDV is suggested on the basis of systemic approach and it reflects the dipole character of electric charge distribution in virion structure and agrees with the virus model, built on the basis of icosahedron symmetry.

Aphthovirus↗
PubMed1984

[Electrical properties of the foot-and-mouth disease virus].

It has been shown that the electron microscopy method can be used for characteristics of the electric properties of foot-and-mouth disease virus. The appearance of simultaneous positive and negative staining during the negative staining of virus preparations with 3-4% PTV solution shows the presence of full virions of constant poles designated as positively and negatively stained areas of protein coat surface. The lateral orientation of virions on the film at routine conditions of preparation and the possibility of virion orientation on the film in the external electrical field allow to characterize the full virions as electric dipoles. It has been suggested that there is a relationship between a virus structure and the character of its electric properties.

Aphthovirus↗
PubMedDate not supplied

[Mechanism of retaining stability of foot-and-mouth disease virus during manufacturing dried concentrated preparations].

Removal of moisture from concentrated virus suspensions in a sequential manner using ultrafiltration, active ventilation and vacuum dehydration of concentrated powders enables us to obtain preparations with a residual content of moisture less than 1%, while the structure and properties of FMD virus are retained. Mechanism of inactivation of FMD virus in drying is attributed to electrophysical processes associated with development of electric potentials in evaporated objects due to directional movement of charged water molecules. Having reached certain values, outer electric potential induces charge exchange in virions that leads to the break of energy balance in the structure of virus particles and to their disruption when rehydrating in buffer solution.

Aphthovirus↗
PubMedDate not supplied

[Effect of the contrast conditions using solutions of phosphotungstic acid on the electron microscopic image of the foot-and-mouth disease virus].

A method of negative staining of foot-and-mouth disease virus preparations permits to obtain separately positive (2% phosphotungstic acid solution, pH 3.0) and negative (2% PTA solution, pH 6.8 +/- 8.0) stainings. When a 3--4% PTA solution, pH 6.8 +/- 8.0 is used, simultaneous positive and negative staining of each virion is possible which characterizes the functional heterogeneity of the virion protein membrane in interaction with PTA anions.

Aphthovirus↗
PubMedDate not supplied

[Physico-chemical bases of the mechanism of thermal inactivation of the foot-and-mouth disease virus].

Experiments demonstrated that the effect of water activity on the solvate complex of virions underlies the mechanism of thermal inactivation of the foot and mouth disease virus in suspension; changes in the structure of the complex impair the electrophysical balance of the RNA-protein relations and hence, destroy the virus. Heating of virus-containing suspensions at moderate positive temperatures leads to destruction of the noninfectious part of virus population of 146S particles, thus decreasing the infectious activity of the virus.

Aphthovirus↗