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Biomedical subjects

A P Pekhov

Publications and source records attributed to A P Pekhov.

At least 19 recordsLinked to original sources
PubMed1993

[Features of expression of the genetic region of plasmid pAP42, controlling the synthesis of "sex" pili and surface exclusion system in various E. coli cells].

The results of investigation of serologically--typed and nontyped E. coli cells carrying F-like derepressed plasmid pAP42 testified to the influence of genome of some bacterial hosts on the expression of plasmid genetic region determining "sex" (plasmid--specific) pili synthesis and surface exclusion system (Sfx--system). Similar changes in bacterial cells phenotype can also result from the mutational changes of this plasmid.

Coliphages↗
PubMed1992

[The genome localization of the plasmid pAP27 fin N system].

Plasmid transfer genetic regulatory system fin N of F-like plasmid pAP27 have been localized on BamHI-restriction fragment f3 (length 8.7 kb). Plasmid pUC19 Fin-activity have been cleared up and characterized from the point of view of its specificity of action on some plasmid transfer functions.

Cloning, Molecular↗
PubMed1992

[The systems of the genetic regulation of plasmid transfer fin K, fin L, fin M and fin N].

F-like plasmids pAP19-1::Tn9, pAP20::Tn9, pAP22-1::Tn1, pAP27 characterized by the presence of unique genetic plasmid transfer regulatory systems in their genomes have been found. These systems were named fin K, fin L, fin M, finN, consequently. They were characterized from the point of view of specificity of their action on F-factor and F-like conjugative function. Dependence of fin N-system expression on host-cell and on the order of plasmid entering into host-cell was shown.

Conjugation, Genetic↗
PubMed1991

[Incompatibility and replication of plasmids].

The identified basic replicons rep1 and rep2 of plasmid pAP42 belong to different groups of incompatibility (inc FIX and inc FVIII). The replicons are partly incompatibile with other inc F-groups too. The results indicate connection between plasmid incompatibility and their replication.

DNA Restriction Enzymes↗
PubMed1991

[Mutations of the genetic region Fin of the F-like plasmid pAP18-1].

With help of nitrosoguanidine 60 mutants of F-like plasmids pAP18-1 drd::Tn 5 and pAP18-1::Tn 9 were induced which determined resistance of E. coli cells of specific phage MS2. Mutational changes in fin-locus of those plasmids were accompanied by phenotypic reversion Fin(-)-Fin+.

Conjugation, Genetic↗