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Biomedical subjects

A Ozkul

Publications and source records attributed to A Ozkul.

24 records · Page 2Linked to original sources
PubMed1998

Effect of intratympanic steroid application on the development of experimental cholesteatoma.

By introducing certain irritants into the middle ear it is possible to produce cholesteatoma. Propylene glycol, the main agent used for this purpose, produces a long-standing inflammation that causes hyperplasia and migration of the epithelium through an intact tympanic membrane. In the present investigation topical prednisolone was used in order to inhibit the production of cholesteatoma. The results indicate that there is a marked decrease in inflammation and hence experimental cholesteatoma production when prednisolone is administered into the middle ear.

Animals↗
PubMed1994

Effect of hydroquinone (HQ) on the development of chick embryos.

The developmental toxic effect of hydroquinone (HQ) was evaluated in chick embryos after 72 and 96 hour of incubation. HQ was injected into the air sacs of the eggs at doses ranging from 0.0625 to 40 micrograms per egg. On the 15th day of incubation, live embryos including controls were removed from the eggs and examined for gross malformations and body hemorrhage. The LD50 values for HQ at 72 and 96 hours of incubation were 8.59 and 15.63 micrograms per egg, respectively. HQ produced gross malformations such as body hemorrhage, curled claws, everted viscera, defective beak, exencephaly and monophtalmia in developing chick embryos at dosages ranging from 20 to 0.0625 micrograms per egg in both groups. The various types of malformations and their incidences in both groups were higher in comparison with the controls, but were not statistically significant. At no concentration did malformations occur greater, than 35 percent for both 72 and 96 hours of incubation. The results of this study reveal that HQ is embryo toxic at higher doses and that teratogenic potential of HQ is unremarkable.

Abnormalities, Drug-Induced↗
PubMed1994

Antibody prevalence of caprine arthritis encephalitis virus (CAEV) in goats in Turkey.

In this study, a serological survey in goat herds for antibodies against Caprine Arthritis Encephalitis Virus (CAEV) in Turkey is described. 808 serum samples were collected from three state farms and seven private flocks in different regions of Turkey. The serologic examination was performed by Agar Gel Immunodiffusion (AGID) technique. 16 (1.9%) out of 808 sera deriving from two state farms (n = 14) and one private herd (n = 2) were found to be serologically positive. The findings are discussed with regard to the relatively low prevalence of CAEV in Turkey, age and race dependency and the kind of origin of the serologically screened animals.

Animals↗
PubMed1993

Cytotoxicity versus antibacterial activity of some antiseptics in vitro.

Through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. A follow-up study was conducted to examine the germicidal effect of 0.05% NaOCl, 10 mg/ml metronidazole and supernatant of Ca (OH)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. In vitro testing revealed that 0.05% NaOCl and Ca (OH)2 were both equally effective on these anaerobes. Also, metronidazole was found to be germicidally effective against Bacteroides melaninogenicus, Bacteroides oralis and Peptostreptococcus anaerobius, but ineffective against Veillonella alcalescens. Furthermore, it was found that NaOCl and Ca (OH)2 had a very destructive effect on cell cultures compared with their antimicrobial effect, whereas metronidazole was less toxic among the agents tested.

Bacillus subtilis↗
PubMed1993

Detection by cultural isolation of bovine virus diarrhoea (BVD) virus following field infections in cattle and their fetuses in Turkey.

Cultural isolation, identification and biotype characterization of BVD virus is based on monolayer tissue culture techniques using mainly organs of, e.g., bovine fetuses. These might have experienced a transplacental transmission and are therefore of no use for tissue cultures produced, e.g., from kidneys of such fetuses. In order to rule out BVD virus infections, fetuses must be tested for BVD virus, viral antigen or specific nucleotide sequences. BVD virus and other ruminant pestiviruses have been detected in Turkey by cultural isolation. In bovine fetuses BVD virus has not been demonstrated so far. Cultural isolation was attempted in the present investigation in 50 randomly selected pregnant cows which were brought to an abattoir. Of these cows serum samples were assayed for neutralizing antibodies to BVD virus and 40 (80%) found positive with titres > or = 1:40. In contrast fetuses 120 days of ontogenic age and therefore supposedly immunocompetent were derived from seropositive dams (n = 16) but did not show any neutralizing antibodies to BVD virus. Leucocyte fractions of peripheral blood samples of the 50 cows as well as 353 fetal blood and organ samples were inoculated and submitted to three passages in fetal calf kidney cell cultures before testing for BVD viral antigen by immunofluorescence technique. BVD virus was thus detected in the fetus of one seropositive cow and also in the leucocyte fraction of one seronegative dam including organs of her fetus. The number of fetuses found positive for BVD virus amongst fetuses collected in abattoirs corresponds well to figures gained by other investigators outside Turkey.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗