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Biomedical subjects

A Orosz

Publications and source records attributed to A Orosz.

At least 37 records · Page 2Linked to original sources
PubMed1988

Alteration of insulin-binding receptors in non-insulin dependent diabetes of the young.

The behaviour of insulin binding receptors is rather unelucidated in non-insulin-dependent diabetes mellitus of the young. Authors in continuing their previous work studied the behaviour of insulin binding receptors of erythrocytes and monocytes in 9 MODY patients. They observed that specific insulin binding of circulating blood cells was significantly decreased in all cases as compared to the controls despite of a good state of metabolism (in the case of erythrocytes 4.63 +/- 1.1% vs. 6.03 +/- 1.7%, p less than 0.05, in the case of monocytes 2.3 +/- 1.2% vs. 3.6 +/- 1.4%, p less than 0.05). The lower value of insulin binding resulted from the decrease of receptor concentrations (in the case of erythrocytes 2.36 +/- 0.78 pmol/l vs. 3.81 +/- 1.14 pmol/l, p less than 0.05).

Adult↗
PubMed1983

Separation of synaptic junctional complexes from thin layers of rabbit cerebral cortex.

Synaptic junctional fractions were separated from rabbit brain by procedures based on combining the methods of Cotman and Taylor [4], Orosz et al. [16, 17] and Lisman et al. [13]. Thin layers of cerebral cortices were homogenized to obtain a crude mitochondrial-synaptosomal fraction. The sedimentation rates of mitochondria and mitochondria containing synaptosomes were increased by raising the density of mitochondria with an insoluble dense formazan deposit inside mitochondria after iodo-nitrotetrazolium treatment. The synaptic plasma membrane fraction isolated by this method contained no mitochondrial contamination. After Triton X-100 treatment the insoluble residues of the detergent were centrifuged through discontinuous sucrose gradients. A great enrichment of morphologically identifiable intact synaptic junctions was observed in some of the obtained interface layers.

Animals↗
PubMed1975

Synaptosomal fractions of cat cerebral cortex after hypo-osmotic and detergent treatment.

Different synaptosomal fractions were prepared by subcellular fractionation from cat cerebral cortex. The seperated nerve ending fractions differed in both density and osmotic sensitivity. Synaptosomal ghosts were obtained by hypo-osmotic treatment. In the synaptosomal ghost fractions the synaptic areas seemed to be intact morphologically. The morphology of the "synaptic triad" (pre- and postsynaptic membranes and the synaptic cleft) was not affected even by treatment with Triton X-100 non-ionic detergent (0.6%). The solubilizing effect of different concentrations of the detergent was checked in the supernatants and in the insoluble residues. The homogeneity of various subcellular fractions and the effect of the osmotic and detergent treatments are discussed.

Animals↗