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Biomedical subjects

A Ono

Publications and source records attributed to A Ono.

At least 253 records · Page 14Linked to original sources
PubMed1984

Effect of sodium saccharin on rat pancreatic enzyme secretion.

Intragastric administration of casein, oleic acid, or polyvinylpyrrolidone resulted in a marked increase in the pancreatic secretion of enzymes, and the administration of 0.03% sodium saccharin, at the concentration permitted in soft drinks, inhibited this increase with these substances. The stimulation of secretion of pancreatic juice-bile mixture by these substances was also inhibited by administration of sodium saccharin. Intravenous injection of CCK-PZ (cholecystokinin-pancreozymin) during inhibition by sodium saccharin, caused a rapid increase in amylase output, suggesting that sodium saccharin acts on the site(s) of CCK-PZ release to prevent its secretion even in the presence of a stimulant, but does not reduce the sensitivity of the pancreas to CCK-PZ. Sodium saccharin seemed to have an effect on secretin release similar to that on CCK-PZ release, judging from the results for the volume of juice-bile mixture.

Amylases↗
PubMed1984

[Quantitation of left-sided valvular regurgitation by gated cardiac blood pool scan: evaluation of a new approach using the Fourier amplitude ratio].

Utilizing the Fourier amplitude ratio (FA ratio), we evaluated a new approach for quantitating valvular regurgitation by the gated cardiac blood pool scan. The subjects consisted of 36 patients with documented left ventricular regurgitation of grade I-IV (group 1), 24 patients with myocardial infarction including severe cardiac dysfunction (group 2), and 15 normal persons (group 3). From each pixel of the gated cardiac blood pool scan, a time-activity curve was obtained and the first-harmonic Fourier analysis was performed. Two functional images; namely, the phase and amplitude images, were generated. To generate the FA ratio, the region of interest was drawn around each ventricle in the amplitude image. The FA ratio was calculated using the following formula; FA ratio = left ventricular amplitude value/right ventricular amplitude value. The FA ratio was 2.66 +/- 1.75 (mean +/- S.D.) in group 1; 1.29 +/- 0.27 in group 2; and 1.11 +/- 0.19 in group 3. The FA ratio for group 1 was significantly higher than those of groups 2 and 3 (p less than 0.001). There was no significant difference between the FA ratios of groups 2 and 3. In patients with regurgitation, the FA ratio was 1.50 +/- 0.37 in patients with grade I, 1.97 +/- 0.34 in grade II, 2.73 +/- 0.95 in grade III; and 6.65 +/- 2.14 in grade IV. There was a good agreement between the FA ratios and quantitative angiographic estimates of regurgitation.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1983

Reye-like syndrome associated with valproic acid.

A case of a Reye-like syndrome during the course of treatment with VPA was reported. Hyperammonemia and severe liver damage as well as diffuse small droplets in the liver biopsy material were demonstrated. On analysis by gas chromatography/mass spectrometry of the urine immediately after the onset, the metabolites of VPA were detected together with lactate and other substances, and electron microscopic observation of liver biopsy material revealed deformation of mitochondria, disappearance of cristae and electron dense deposits in the matrix.

Adolescent↗
PubMed1983

Chymotryptic subfragments of troponin T from rabbit skeletal muscle. Interaction with tropomyosin, troponin I and troponin C.

The binding of the chymotryptic troponin T subfragments to tropomyosin, troponin I, and troponin C was semiquantitatively examined by using affinity chromatography, and also by co-sedimentation with F-actin and polyacrylamide gel electrophoresis in 14 mM Tris/90 mM glycine. Circular dichroism spectra of the subfragments were measured to confirm that the subfragments retained their conformational structures. Based on these results, the binding sites of tropomyosin, troponin I, and troponin C on the troponin T sequence were elucidated. Tropomyosin bound mainly to the region of troponin T1 (residues 1-158) with the same binding strength as to the original troponin T. The C-terminal region of troponin T (residues 243-259) was the second binding site to tropomyosin under physiological conditions. The binding site of troponin I was concluded to be the region including residues 223-227. The binding of troponin C was dependent on Ca2+ ion concentration. The C-terminal region of troponin T2 (residues 159-259) was indicated to be the Ca2+-independent troponin C-binding site and the N-terminal side of troponin T2 to be the Ca2+-dependent site.

Animals↗
PubMed1983

Difference in the inductions of duodenal alkaline phosphatase by casein feeding and by starch feeding.

Feeding of starch or casein to rats that had been starved for 4 days resulted in marked increase in duodenal alkaline phosphatase activity in a few hours. In rats with a duodenal blind loop, the enzyme was induced by feeding starch, but not casein. Conditions leading to hyperglycemia, such as an intragastric administration and an intravenous infusion of glucose, induced duodenal alkaline phosphatase to the same extent and in the same period as starch feeding. Subcutaneous injection of insulin alone did not induce the enzyme. In chemically sympathectomized rats, the enzyme was induced by starch feeding. In vagotomized or hexamethonium-treated rats, the enzyme was not induced by starch feeding or intragastric administration of glucose solution. On the contrary, subcutaneous injection of carbachol alone induced the enzyme. These results suggest that stimulation of the parasympathetic nervous system via hyperglycemia is important for induction of duodenal alkaline phosphatase by starch feeding. The mechanism of enzyme induction by casein feeding is still unknown.

Alkaline Phosphatase↗
PubMed1983

Memory of the rhythmic change in activity of duodenal alkaline phosphatase in rats.

Studies were made on memory of the rhythmic change in activity of duodenal alkaline phosphatase in rats. During starvation, the peak enzyme activity decreased gradually disappearing in 4 days. The peak activity of duodenal alkaline phosphatase was retained by feeding starch diet for 4 days instead of starvation for 4 days, but not by feeding casein diet for the same period. Starvation for one day after feeding casein diet for 4 days resulted in disappearance of the peak activity. However, the peak activity was still retained after one day of starvation after 4 days on starch diet. Therefore, starch feeding appears to be important in the memory of the rhythmic change in activity of duodenal alkaline phosphatase.

Alkaline Phosphatase↗
PubMed1983

RNA tumor virus-like particles in human fetal thymus cells stimulated by human B-cells.

Human fetal thymuses were investigated in a search for retroviruses. By electron microscopy, retrovirus-like particles were detected in the thymus cells only when they were cocultured with mitomycin C-treated human B-cells. Reverse transcriptase activity in the culture medium was found at a density of 1.15-1.17 g/cm3 in sucrose density gradients.

B-Lymphocytes↗
PubMed1982

[Measurement of left-sided valve regurgitation by gated cardiac blood pool scans using stroke volume imaging].

Radionuclide gated cardiac blood pool scan was used to quantify the left-sided valvular regurgitation by calculating the ratio of left ventricular to right ventricular stroke counts (the stroke count ratio) obtained directly from the "stroke volume image." The subjects consisted of 21 patients and 11 control subjects. In 21 patients, contrast angiography disclosed that 10 had isolated mitral regurgitation, nine had isolated aortic regurgitation and two had combined mitral and aortic regurgitation. The degree of aortic and/or mitral regurgitation was judged using the Sellers' criteria. The stroke count ratio was 1.34 +/- 0.21 (mean +/- SD) in a group without regurgitation, 1.73 +/- 0.13 in a group of patients with grade I, 2.14 +/- 0.19 in grade II, 2.76 +/- 0.58 in grade III and 5.04 +/- 0.27 in grade IV regurgitation. There was a good agreement between the stroke count ratios and quantitative angiographic estimates of regurgitation. Furthermore, the use of the "stroke volume image" (obtained from the subtraction of end-systolic from end-diastolic images) allowed a simplified drawing of the region of interest and avoided the problem in selecting the background. For its simplicity, we found little interobserver and intraobserver variations (r = 0.98 in the former and r = 0.99 in the latter). Therefore, this method permits a noninvasive and reliable assessment of the severity of left-sided valvular regurgitation, and has a great potential clinical utility in the long-term follow-up of asymptomatic patients with regurgitation, and in the evaluation of the results of medical and surgical interventions.

Aged↗