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Biomedical subjects

A Oda

Publications and source records attributed to A Oda.

At least 109 records · Page 6Linked to original sources
PubMed1984

[Intrathoracic movement of the normal and hypertrophied hearts measured by biplane coronary cineangiography].

The shift of the heart during systole within the thorax was measured using bifurcations of the left coronary artery as cineangiographic markers. Biplane coronary cineangiography was performed in 13 normal subjects and 6 patients with non-obstructive hypertrophic cardiomyopathy (HCM). The spatial coordinates (X, Y, Z) of the bifurcations on the cineangiograms were measured using a motion analizer-digitizer-computer system. The systolic excursion of the motion of a bifurcation located at the anterior-basal point of the heart was 1.4 +/- 0.1 (+/-SD) cm leftward, 3.0 +/- 0.3 cm caudally and 2.5 +/- 0.1 cm anteriorly in normal subjects. In the cases with HCM, on the other hand, the bifurcation moved 2.2 +/- 1.1, 2.7 +/- 1.2 and 2.2 +/- 0.6 cm during systole, respectively. The movement at the apex in the normal subjects was 1.7 +/- 0.2 cm rightward, 1.5 +/- 0.2 cm caudally and 1.5 +/- 0.2 cm posteriorly, although the direction was reversed as compared to that of the anterior wall of the cardiac base. The amplitude of the excursion was also reduced at the apex, suggesting the systolic twist of the ventricular wall. The excursion of the apex in HCM was 0.6 +/- 1.7, 1.5 +/- 1.8 and 2.5 +/- 1.4 cm, respectively, toward the base of the heart as in the normal subjects. The maximum speeds of these motions were 34.0 +/- 9.2 cm/sec leftward, caudally and anteriory at the anterior-basal point and 36.2 +/- 7.3 cm/sec rightward, caudally and posteriorly in the normal subjects.(ABSTRACT TRUNCATED AT 250 WORDS)

Angiography↗
PubMed1983

Detection of viral antigens in formalin-fixed specimens by enzyme treatment.

Enzyme treatment (protease or trypsin) was applied to formalin-fixed paraffin-embedded materials and virus-infected cultured cells to detect viral antigens by immunofluorescence. The viral antigens were demonstrated in several organs of autopsy or biopsy cases of which diagnoses had been established by immunofluorescence or virus isolation using frozen materials, or suspected on the basis of serology and/or histopathological findings. These included herpes simplex, varicella-zoster, cytomegalo, subacute sclerosing panencephalitis, progressive multifocal leukoencephalopathy, Japanese B encephalitis, measles, acute hemorrhagic conjunctivitis, Lassa and Korean hemorrhagic fever. Antigen could be recovered also in virus-infected cells (herpes simplex, measles, Lassa, Ebola, Marburg, Rift Valley, Congo and Korean Hemorrhagic fever) by enzyme treatment after periods of formalin fixation of four weeks and storage of three months. In herpes simplex virus-infected mouse brain, antigen was detected after fixation for three months in formalin.

Adolescent↗
PubMed1983

[Characteristics of left ventricular regional wall relaxation at the infarction site].

In order to investigate relaxation characteristics of the infarcted wall, segment lengths of the left ventricular wall were calculated in 6 patients with anterior myocardial infarction and in 9 angiographically normal subjects. The biplane left coronary cineangiography was performed and coordinates of ramifying points of the left coronary artery on the infarcted or non-infarcted wall were measured and the spatial lengths (segment lengths) on each wall were calculated. If the two points are on the left anterior descending artery (LAD) in infarcted patients, a calculated length is regarded as a segment length in the infarcted portion, while if the length is calculated between the two points located on the coronary artery other than LAD, it is a non-infarcted segment. Each segment length was calculated in frame by frame covering one cardiac cycle. The maximum length (Lmax), minimum length (Lmin) and the length at the beginning of P wave of the electrocardiogram (LECG P) were calculated. The extent of relaxation of the segment caused by atrial kick was calculated as (Lmax--LECG P)/Lmax X 100, (% delta Lla). This value was 2.8 +/- 1.5 and 3.5 +/- 1.4% on anterior and inferior walls, respectively, in 9 normal subjects and 3.6 +/- 1.2 and 4.0 +/- 0.9% in 6 patients with anterior infarction, revealing no significant difference among 4 values. This indicates that the amount of lengthening of the segment by atrial kick remains unchanged even if the wall is infarcted. The extent of diastolic lengthening of the segment by rapid and slow fillings, which excludes atrial filling, was calculated as (LECG P--Lmin)/Lmax X 100, (% delta L1-la).(ABSTRACT TRUNCATED AT 250 WORDS)

Cineangiography↗
PubMed1982

Enzymatic treatment of formalin-fixed and paraffin-embedded specimens for detection of antigens of herpes simplex, varicella-zoster and human cytomegaloviruses.

Attempts were made to detect antigens of herpes simplex (HS), varicella-zoster (VZ) and human cytomegalo (CM) viruses in pathological preparations made by formalin fixation and paraffin embedding as well as in infected culture cells by means of enzymatic treatment followed by indirect immunofluorescent staining, and the following results were obtained. 1) Specimens from autopsy cases, in which diagnosis was established by detection of viral antigens or virus isolation, showed specific antigens in specimens of various organs of all cases of HS (6 cases), VZ (3 cases) and CM (2 cases). In 17 other cases, which were suspected for those viral infections on the basis of ordinary light microscopic observation, also demonstrated respective viral antigens specifically, enabling differential diagnosis. In one case, HS antigen was detected in the esophagus and CM antigen in the liver, testifying occurrence of a double infection. 2) The time spun during which the specific antigens could be preserved was tested, and it was learned that preservation of stainable antigens was better in paraffin-embedded specimens than in specimens kept in formalin. It was possible to detect HS and VZ antigens in paraffin-embedded specimens prepared 20 and 18 years ago, respectively. 3) The optimal conditions of trypsin treatment varied depending upon the organ as well as upon each individual specimen, but a commonly recommendable procedure was found to by warming at 37 degrees C for 3 hours with 0.25% trypsin. A test with HS virus-infected Vero cells indicated that the effect of trypsin treatment was influenced by the concentration of formalin and the time of fixation.

Antigens, Viral↗
PubMed1982

[Diagnosis of idiopathic mitral valve prolapse by two-dimensional echocardiography: evaluation of its clinical significance in the prospective follow-up study].

One hundred and five cases with idiopathic mitral valve prolapse (MVP) diagnosed by two-dimensional (2-D) echocardiography were classified into 5 groups according to the grade of prolapse (Fig. 1), 47 cases of which were followed prospectively for average 2.9 years. 1) Most of the cases with MVP of grade 3 or less showed normal left ventricular diastolic dimension (LVDd) and left atrial dimension (LAD) throughout the follow-up period, while the majority of the cases with MVP of grade 4 revealed increased LVDd and LAD. Thus the mitral regurgitation due to MVP seemed to be insignificant in cases of grade 3 or less, while it is significant in cases of grade 4. 2) During the follow-up period cases of grade 1, 2 and 3 showed the transition of the grade of prolapse each other. On the other hand, most of the cases of grade 4 at the initial examination did not show the change in its grade of prolapse. 3) Fifty-eight cases (55.2%) had symptoms such as chest pain, palpitation and dizziness, and various electrocardiographic abnormalities such as ST and T wave changes, prolongation of QT interval and arrhythmias were also frequently observed. However, the incidence of these symptoms and signs was independent from the grade of MVP. It was concluded that MVP with grade 4 or more is hemodynamically significant, while it was insignificant in cases of grade 3 or less. Therefore, those with symptoms and/or electrocardiographic abnormalities in cases with MVP of grade 3 or less will not require any treatment but only a follow-up observation.

Adult↗
PubMed1979

Diagnosis and classification of the mitral valve prolapse by the ultrasoundcardiotomography and the evaluation of the M-mode technic.

Eighty cases with mitral valve prolapse excluding the secondary prolapse of the mitral valve caused by known underlying diseases were studied by real-time ultrasoundcardiotomography and M-mode technic. It was thought that observation of the left ventricle with long axis sector scan was useful and sensitive technic to diagnose the mitral valve prolapse. By comparative study of M-mode technic and ultrasoundcardiotomography, echo sources and the mechanism of so called prolapse patterns such as pansystolic bowing, mid-systolic buckling and multilayered echoes were explained. Pansystolic bowing and mid-systolic buckling were considered as the reliable signs for diagnosis of anterior leaflet prolapse, but were not contributory to diagnose posterior leaflet prolapse and ultrasoundcardiotomographic technic was needed to detect the posterior leaflet prolapse. According to the mode of prolapsing findings by ultrasoundcardiotomograms classification of severity of mitral valve prolapse was undertaken and its grade was expressed as AmPn in which A and P designated prolapse of the anterior and posterior leaflet respectively and m and n indicated the grade of severity in number from zero to five.

Adolescent↗