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A NOWOTNY

Publications and source records attributed to A NOWOTNY.

At least 19 recordsLinked to original sources
PubMed1964

RELATIONSHIP OF STRUCTURE TO FUNCTION IN BACTERIAL O ANTIGENS. III. BIOLOGICAL PROPERTIES OF ENDOTOXOIDS.

Johnson, Arthur G. (The University of Michigan, Ann Arbor), and Alois Nowotny. Relationship of structure to function in bacterial O antigens. III. Biological properties of endotoxoids. J. Bacteriol. 87:809-814. 1964.-Endotoxin (somatic O antigen) was rendered much less lethal for mice and rabbits after treatment with boron trifluoride, potassium methylate, or pyridinium formate. In addition, the ability to induce the second fever peak of the biphasic febrile response characteristic of endotoxin was abolished. The capacity to prepare for the local Shwartzman reaction was also reduced after such treatments. On the other hand, stimulation of nonspecific resistance, and adjuvant action in mice was retained. The ability to elicit specific antibody was diminished after boron trifluoride treatment but only slightly retarded by the other two procedures.

Animals↗
PubMed1963

Relation of structure to function in bacterial O antigens. I. Isolation methods.

Nowotny, Anna M. (City of Hope Medical Center, Duarte, Calif.), Scott Thomas, Olga S. Duron, and Alois Nowotny. Relation of structure to function in bacterial O antigens. I. Isolation methods. J. Bacteriol. 85:418-426. 1963.-Six isolation methods, four previously described and two reported herein, were compared for the extraction of endotoxic O antigens from three different enterobacteria: Serratia marcescens Bizio, Salmonella typhosa O901, and Escherichia coli K-12 lambda. Large variations were observed in the toxin yield, in the serologically reactive material yield, and in the nucleic acid-releasing abilities of the six methods when applied to the different bacterial strains. Chemical analyses of the toxic O antigens included quantitative determination of the amino acids, long- and short-chain carboxylic acids, and carbohydrates. Comparisons of the nucleic acid content were also made. Although the chemical composition of the preparations was quite different, no correlation could be found between the percentage of the above components and the lethal toxicity or the serological reactivity. It was concluded that the structural elements or properties responsible for these biological activities cannot be detected by gross chemical analyses.

Antigens↗
PubMed1963

Relation of structure to function in bacterial O antigens. II. Fractionation of lipids present in Boivin-type endotoxin of Serratia marcescens.

Nowotny, Alois (City of Hope Medical Center, Duarte, Calif.). Relation of structure to function in bacterial O antigens. II. Fractionation of lipids present in Boivin-type endotoxin of Serratia marcescens. J. Bacteriol. 85:427-435, 1963.-Methods for liberation of lipids from their endotoxic O antigen were investigated. In the case of endotoxin isolated from a chromogenic Serratia marcescens, best results were obtained with diluted formic acid. The crude lipid mixture consisted of at least 16 different components, which were analyzed by paper chromatography. Good separation was achieved by silicic acid-impregnated paper. Lipids were stained with metachromatic o-toluidine blue. Solubility of the crude lipid mixture was studied in different organic solvents. Preparative fractionation was developed utilizing various organic solvents on the basis of solubility differences of the lipid components. Pure fractions were obtained by silicic acid column chromatography. Since none of the lipid liberation methods so far developed can produce undegraded lipids free from split products and remnants of cell-wall polysaccharides, their usefulness is limited in comparing the chemical structure of the liberated lipid with that of the lipid intact in the cell wall. The liberation procedures cause changes within the lipid structure itself, which in turn would alter whatever potential ability it might have to elicit certain biological reactions.

Antigens↗