Search PubMed⌕ Search

Biomedical subjects

A N Shutko

Publications and source records attributed to A N Shutko.

35 records · Page 2Linked to original sources
PubMedDate not supplied

[Informational activity of exogenous DNA in irradiated cells].

The levels of total DNA, RNA and protein synthesis were examined in control and in vivo irradiated thymocytes after their preincubation with exogenous DNA of different polymerization degree. The studies were made using the uridine-14C and lysine-14C. The influence of preliminary treatment of exogenous DNA or cells with actinomycin D (AMD) was studied as applied to the levels of their own and "induced" synthesis in the cells. It is shown that native exogenous DNA induced the increase in the synthesis of all three components only in the irradiated cells. The pretreatment of the irradiated cells or exogenous DNA with AMD completely inhibits the "induced" synthesis of RNA and protein while their own synthesis in suppressed only by 20%. Possible transcription and translation of highly polymerized exogenous DNA in the irradiated cells without its integration into the genome of the "host" is discussed.

Animals↗
PubMedDate not supplied

[On cytocidic effect of exogenous modified DNA].

The number of defects of the secondary structure and dispersions of the molecular weights of DNA preparations were studied before and after their gamma-irradiation within a 5-2500 krad range of doses. These results are compared with the ability of the irradiated preparations to induce death of thymocytes irradiated in vivo during their combined incubation in vitro. It is established that the DNA biological activity is a result of the presence in the total preparation of molecule fractions of 5.10(6) or 1.10(6) Daltons with 3 or 50-80 defects of the secondary structure.

Animals↗
PubMedDate not supplied

[Kinetics of lymphoid cell subpopulations in the mouse spleen in the early period after unilateral nephrectomy].

The content of total T- and B-lymphocytes, T-rosette-forming at 37 degrees, T-autorosette-forming (ARF), FcIgM+, FcIgG+ and TDT+ lymphocytes is determined in mice after unilateral nephrectomy. The number of ARF, FcIgM+ and TDT+ lymphocytes increased 17 and 48 hours after the nephrectomy. The coincidence in time of the lymphocyte population increase with acquisition of morphogenetic activity by the spleen lymphoid cells allows one to assume their participation in the regenerative proliferation of renal epithelium. The alterations in the content of other lymphocyte populations were in the limit of random deviations.

Animals↗