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Biomedical subjects

A N Ilialetdinov

Publications and source records attributed to A N Ilialetdinov.

At least 19 recordsLinked to original sources
PubMedDate not supplied

[Participation of heterotrophic microorganisms in the purification of drainage waters from heavy metal ions].

Heterotrophic microorganisms can grow in the presence of toxic components contained in industrial drainage of non-ferrous metal industry. The content of microorganisms correlates with the concentration of organic substances of plant origin in drainage waters. In the course of mineralization, heavy metal ions are immobilized and precipitated as stable metal-organic complexes with microbial metabolites. The rate of metal precipitation increases in the presence of aerobically decomposed plant material. The results obtained can be employed for purification of drainage waters from metal ions.

Bacteria↗
PubMedDate not supplied

[Immobilization of microorganisms on latex for producing artificial flocs].

Microorganisms belonging to the genera Pseudomonas, Micrococcus, Bacillus and Rhodotorula are flocculated with divinyl styrene latex. The process of flocculation depends on a number of physico-chemical factors but, all in all, is universal for microorganisms with different taxonomy, morphology and physiology. The structure of artificial bioflocs has been studied using optical and scanning electron microscopy.

Bacteria↗
PubMedDate not supplied

[Oxidation of alpha-methylstyrene by Pseudomonas cultures].

The work was aimed at studying alpha-methylstyrene oxidation by three active Pseudomonas cultures. P. acidovorans 9 oxidized alpha-methylstyrene only to acetophenone whereas two P. aeruginosa cultures assimilated the compound entirely. Analysis of the intermediate products and enzymes catalyzing the aromatic ring cleavage suggests that these strains oxidize alpha-methylstyrene via a new unknown pathway.

Acetophenones↗
PubMedDate not supplied

[Glucose consumption and dehydrogenase activity of the cells of the arsenite-oxidizing bacterium Pseudomonas putida].

The rates of glucose assimilation and dehydrogenase activity were studied in Pseudomonas putida oxidizing arsenite. The rate of glucose utilization by the cells decreased in the presence of arsenites in the medium at the beginning because of the microbial adaptation to arsenite. The activity of dehydrogenase fell down when the cells were cultivated in the medium with arsenite. An inverse correlation existed between the rate of glucose assimilation and arsenite oxidation. Apparently, arsenites were oxidized under the action of metabolites produced by P. putida in the process of its heterotrophous growth.

Arsenic↗
PubMedDate not supplied

[Bacterial destruction of synthetic organic flotation agents].

Microorganisms that use the flotation agent T-66 as the sole source of carbon have been isolated from soil enriched with this agent. A mixture of bacterial cultures belonging to the genus Pseudomonas (Ps. fluorescens, Ps. desmolyticum, Ps. rathonis, Ps. cyanoides viscosa, and Ps. aeruginosa) oxidized, after adaptation, at a high rate, components of the flotation agent T-66. Aeration of the medium accelerated the destruction. About 90% of the foltation agent components were oxidized within four months. The highest activity was observed during the first two months. The bacteria also decomposed, actively, oxidized kerosene and oxidized recycle stock added as the sole source of carbon to a mineral medium. Introduction of glucose to the medium accelerated destruction of oxidized recycle stock by the microorganisms, but inhibited destruction of oxidized kerosene.

Adaptation, Physiological↗
PubMedDate not supplied

[Microbiological leaching of zinc and lead ores of the Tekeli deposit].

In certain regions of the West Tekeli lead-zinc ore deposti, the number of Thiobacillus ferrooxidans cells reaches 10(6) per 1 ml of ore water. Spontaneous bacterial leaching of sulphide ore is confirmed by an increase in the concentration of ferric iron and zinc in ore water. As a result of this leaching, up to 40 per cent of zinc and 60 per cent of lead have been leached from lead-zinc ore within 271-286 days. Bacterial leaching of lead intermediate products has shown that the maximum rate of galenite oxidation is reached at 50--65 degrees C and the content of Fe3+ in a solvent being above 20 g per litre. Microbiological leaching can be used for selective extraction of zinc and lead. Zinc dissolves directly in the leaching bacterial solution while lead remains in the precipitate and is then dissolved under the action of concentrated NaCl solutions.

Lead↗
PubMedDate not supplied

[Activity of microorganisms in activated sludge in relation to the concentration of mercury in sewage].

Activated sludge from the refinery of a plant producing synthetic rubber was shown to adsorb mercury ions contained in the sewage. As a result, the content of microorganisms in the sludge as well as the activity of dehydrogenase decreased. The quantity of Pseudomonas aeruginosa cells was 40 x 10(6) per 1 ml in a chemically defined medium without mercury ions, 20 x 10(5) per 1 ml in the water after the first sedimentation tank (0.07 to 0.08 mg Hg per litre), and 10 x 10(6) per 1 ml in the water of sewage common to the whole plant (0.13 to 0.14 mg Hg per litre). The use of the phage-resistant P-aeruginosa T-76 culture adapted to synthetic organic pollutants contained in the sewage of the plant together with the activated sludge of the refinery increased the biological activity of the sludge and improved the quality of purification in terms of dehydrogenase activity and chemical uptake of oxygen.

Chemical Industry↗
PubMedDate not supplied

[Bacteria decomposing alpha-methylstyrol].

Two bacterial strains assimilating alpha-methylsterene as a sole carbon and energy source were isolated from the sewage of an industrial plant producing synthetic rubber. The morphological, cultural and physiologo-biochemical properties of the strains were studied and their taxonomic position was determined. One of the cultures was classified as Bacillus cereus and the other as Pseudomonas aeruginosa. The rate of alpha-methylsterene destruction in the chemically defined medium was shown to depend on the conditions of cultivation.

Air Pollutants, Occupational↗
PubMedDate not supplied

[Phosphate and glucose accumulation by Pseudomonas cultures in relation to their arsenic resistance].

The effect of arsenite and arsenate on 14C-glucose and 32-P-phosphate transport was studied in the cells of Pseudomonas aeruginosa 561 sensitive to arsenite and in the cells of Pseudomonas putida 18 oxidizing arsenite and resistant to arsenic. Transport and accumulation of phosphate and glucose were inhibited in the presence of arsenite in the cells of P. aeruginosa 561 whereas arsenate inhibited only phosphate accumulation. Arsenite and arsenate had hardly any effect at the initial transport rate and on the overall accumulation of phosphate and glucose in the cells of P. putida 18. The resistance to arsenite is supposed to be caused by selective impermeability of the cellular membranes to arsenite and arsenate.

Arsenic↗
PubMedDate not supplied

[Catalase activity of a Pseudomonas putida strain oxidizing arsenic].

Trivalent arsenic ions added to the medium stimulated the catalase activity of a Pseudomonas putida 18 culture correlating with the rate of arsenic oxidation. As soon as the process of arsenic oxidation stopped, the activity of catalase decreased. Apparently, the production of H2O2, its decomposition by catalase and oxidation of As(III) to As(V) by the formed oxygen are a device for detoxication of trivalent arsenic acquired by the cell in the course of evolution. Therefore, oxidation of trivalent arsenic by the culture involves the peroxide mechanism.

Arsenic↗
PubMedDate not supplied

[Arsenic oxidation by the heterotrophic bacteria Pseudomonas putida and Alcaligenes eutrophus].

Two heterotrophic bacteria, Pseudomonas putida 18 and Alcaligenes eutrophus 280, were isolated from gold-arsenic deposits. The bacteria oxidize As(III) to As(V) at pH 6-9 and temperatures 4-28 and 28 degrees C respectively. The oxidation is accompanied by a decrease in the pH of the medium. The rate of the oxidation directly depends on the number of cells in the inoculum.

Alcaligenes↗
PubMedDate not supplied

[Autotrophic arsenic oxidation by a Pseudomonas arsenitoxidans culture].

A microorganism oxidizing As(III) to As(V) was isolated from the water of a gold-arsenic deposit; the process occurs under the autotrophic conditions. The microorganism is a motile Gram-negative rod with one flagellum. It does not assimilate organic carbon sources and grows in a mineral medium with 1.3 g of As(III) per litre and acidification of the medium from the pH 7.5-8 to 4.5. The source of nitrogen for the growth is ammonium salts, the source of phosphorous is KH2PO4. It can assimilate up to 41.2-41.7% of the carbon from carbonates as was shown using labeled carbon. The organism is capable of changing the crystal structure of arsenopyrite. In its physiologo-biochemical properties, the organism does not resemble any species described by Bergey (1975) and therefore referred to as Pseudomonas arsenitoxidans.

Arsenic↗
PubMedDate not supplied

[Participation of sulfate reducing bacteria in copper precipitation].

Chemical precipitation of copper by hydrogen sulphide at three values of pH (3.0; 5.0; 7.0) did not result in complete elimination of the metal from a solution. If sulphate reducing bacteria and a source of organic substance, for instance, disintegrated reed, are introduced into the medium, the microorganisms begin to grow, the redox potential decreases, hydrogen sulphide is formed, and copper is completely eliminated from a solution within 10--15 days in model experiments.

Chemical Precipitation↗