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Biomedical subjects

A Mukherjee

Publications and source records attributed to A Mukherjee.

At least 289 records · Page 16Linked to original sources
PubMed1980

Oxidative phosphorylation in isolated canine myocardial mitochondria. Effects of in vitro volume dilution, lactate, phosphate, and calcium addition, and lactic acidosis.

Oxidative phosphorylation of isolated canine myocardial mitochondria was evaluated after exposure to different concentrations of phosphate (5-50 mM), lactate ion in excess (5-40 mM, pH 7.4), and calcium (50-200 microM), to lactic acidosis (pH 6.3), and to mitochondrial protein dilution (in vitro volume expansion) for 10 min to 8 hr. The influence of phosphate and lactate ion addition, lactic acidosis, and in vitro volume expansion on mitochondrial function were studied in the isolation medium (0.18 M KCl, 0.5% bovine serum albumin (BSA) with or without Tris-EDTA, pH 7.4) prior to evaluation of mitochondrial function in the assay medium (0.25 M sucrose, 10 mM Tris-HCl, and 10 mM inorganic phosphate, pH 7.4). The effect of calcium addition was assessed in the assay medium. The results of these studies demonstrate that each of these interventions detrimentally alters mitochondrial oxidative phosphorylative ability. The most severe mitochondrial function impairment resulted from phosphate (5-50 mM) or calcium (50 or 200 microM) addition, each of which decreased the rate of oxygen consumption by 40% or greater. The mitochondrial functional alterations resulting from the interventions that were studied persisted after their removal, but the detrimental effect of phosphate addition and in vitro volume expansion was partially corrected by the addition of cytochrome c. The data are consistent with the possibility that each of these interventions is capaable of altering mitochondrial function but suggest that phosphate and calcium ion accumulation exert the most important detrimental effect on oxidative phosphsorylation in isolated canine cardiac mitochondria.

Acidosis↗
PubMed1980

An improved embedding method for electron microscopy of lepromata.

Spurr's resin mixture has been found to be the embedding material most suitable for electron microscopic studies of lepromata at present. Like methacrylate, it can penetrate well into foamy structures and the leprosy bacilli within foamy structures. On the other hand, like Epon 812, it is stable against the electron beam and can preserve ultrafine structures. Additionally, we have found that the use of dimethylformamide or dimethylsulfoxide instead of propylene oxide as substituter improves the preservation of the ultrafine structures of leprosy bacilli.

Absorption↗
PubMed1980

Leprous phlebitis.

Six cases of phlebitis of leprous origin affecting the superficial veins have been recorded. This lesion does not seem to be as rare as was previously believed. The clinical and histopathological details of these cases are presented.

Humans↗
PubMed1980

Studies on human heart citrate synthase.

Citrate synthase from human heart was purified by affinity chromatography with Sepharose-ATP. The molecular weight (100,000) and the presence of two presumably identical subunits do not differ from other mammalian citrate synthases. However, the kinetics constants and immunologic characteristics of the enzyme differed from other mammalian citrate synthases. The Km values for acetyl-CoA (0.4 microM) and oxaloacetate (0.25 microM) were about an order of magnitude lower than those previously found for other mammalian (and eucaryotic) citrate synthases. The kinetics constants for the reverse reaction Km for citrate (420 microM) and CoA (70 microM), were of similar magnitude to the values for other mammals. Anti-human heart antiserum developed a single precipitin line in an Ouchterlony plate against a heart extract, no precipitin line with brain, and a precipitin line with spurs against liver and kidney extract. Following myocardial infarction in men, the enzyme appeared in peripheral blood rarely and in low concentration in contrast with earlier experiences with experimental infarction in dogs.

Animals↗
PubMed1980

A bacteriological and histopathological study of apparently normal skin in lepromatous leprosy.

A comparative study of clinically affected and apparently uninvolved skin in lepromatous patients has been undertaken in 22 cases. Parameters studied include skin smears for bacillary Index; bacillary load/gm of tissue and histopathological comparison of granuloma fraction and biopsy index. The results showed that the clinically unaffected sites have a lower bacillary index and lesser bacterial load. Histologically the granulomas were smaller and biopsy index was lower in uninvolved areas. The possible reasons for this comparative less involvement are discussed.

Adolescent↗
PubMed1979

Influence of volume dilution, lactate, phosphate, and calcium on mitochondrial functions.

Oxidative phosphorylation of isolated canine myocardial mitochondria has been evaluated after exposure to different concentrations of phosphate (5--50 mM), lactate ion in excess (5--40 mM, pH 7.4), calcium (50--270 nmol/mg protein), to lactic acidosis (pH 6.3), and to mitochondrial protein dilution (in vitro volume expansion) for 10 min to 8 h. The influence of phosphate and lactate ion addition, lactic acidosis, and in vitro volume expansion on mitochondrial function were studied in the isolation medium (0.18 M KCl, 0.5% BSA (bovine serum albumin), with or without Tris-EDTA, pH 7.4) prior to evaluation of mitochondrial function in the assay medium (0.25 M sucrose, 10 mM Tris-HCl, and 10 mM inorganic phosphate, pH 7.4). The effect of calcium addition was assessed in the assay medium. The results of these studies demonstrate that each of these interventions detrimentally alters mitochondrial oxidative phosphorylative ability. The most severe mitochondrial functional impairment resulted from phosphate or calcium addition. The detrimental effect of phosphate and in vitro volume expansion was partially corrected by the addition of cytochrome c.

Animals↗
PubMed1979

Beta adrenergic and muscarinic cholinergic receptors in canine myocardium. Effects of ischemia.

Experimental myocardial ischemia produced in dogs by proximal left anterior descending coronary artery ligation is accompanied by relatively rapid (1 h) increases in the number of (-) [3H]dihydroalprenolol binding sites without changing their dissociation constants in ischemic left ventricular tissue. The changes, persist for at least 8 h and are accompanied by marked decreases in myocardial tissue ischemic region norepinephrine content. In contrast, in the same canine model 1 h of proximal left anterior descending coronary artery ligation did not result in a significant change in the number of [3H]quinuclidynl benzilate binding sites of their dissociation constants. However, the number of [3H]quinuclidynl benzilate binding sites (muscarinic cholinergic receptors) are 50--70% greater than (-) [3H]dihydroalprenolol binding sites (beta adrenergic receptors) in canine left ventricular tissue. Thus, the data suggest that proximal left anterior descending coronary artery occlusion for 1 h significantly increases the number of beta adrenergic receptors in ischemic left ventricular tissue without changing the number of muscarinic cholinergic receptors. Whether the ischemia-produced increase in cardiac beta-receptor content is causally related to increased cyclic AMP levels that develop in ischemic tissue and/or an etiologic factor in arrhythmias originating from ischemic myocardial tissue will have to be determined in additional studies.

Animals↗
PubMed1978

Creatine kinase BB isoenzyme levels by radioimmunoassay in patients with neurological disease.

Creatine kinase BB (CK-BB) isoenzyme was measured by radioimmunoassay in serum and in cerebrospinal fluid from 61 patients with various neurological disorders. Statistically significant elevations of CK-BB isoenzyme in the serum were observed in patients with acute cerebrovascular accidents and in those with seizures and a prolonged alteration in level of consciousness. Statistically significant elevations of CK-BB isoenzyme in the cerebrospinal fluid were also found in patients who had suffered acute cerebrovascular accidents. Some patients with central nervous system infections, acute demyelinating disease, certain drug overdoses, head trauma, and complex migraine also had elevations of serum and CSF CK-BB isoenzyme. In 2 patients with elevations of CK-BB isoenzyme, the elevations in serum occurred later than those detected in the cerebrospinal fluid. If CK-BB elevations determined by radioimmunoassay can be demonstrated to be quantitatively related to the extent of brain damage, then these determinations in conjunction with experimental animal models and newer radiological techniques should allow evaluation, in an objective and precise manner, of measures designed to decrease that damage.

Brain Damage, Chronic↗
PubMed1978

Regulatory enzymes of carbohydrate and energy metabolism in the rabbit blastocyst.

The activities of phosphofructokinase, pyruvate kinase, citrate synthase and creatine kinase were determined in blastocysts from rabbits at 144 h post coitum and in similar blastocysts cultured for 24 h with or without oestradiol-17beta (1 microgrm/ml). There was a significant increase in all the enzymes during the 24-h culture period but oestradiol had no effect.

Animals↗