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Biomedical subjects

A Meybeck

Publications and source records attributed to A Meybeck.

28 records · Page 2Linked to original sources
PubMed1991

Paf-acether in human skin.

Paf is a phospholipid mediator present in human skin which induces inflammatory events, such as neutrophil infiltration and increased vascular permeability. Recent data suggest that cutaneous cells, such as fibroblasts and keratinocytes, produce paf and that paf is released during allergic cutaneous reactions. It is tempting to speculate that paf may contribute to the development of various skin disorders with acute and chronic skin inflammation. Paf antagonists may help in bringing answers to this hypothesis and may offer new prospects for the treatment of cutaneous inflammatory diseases.

Humans↗
PubMed1989

Participation of the membrane effect to the cytotoxicity of tensio-active agents comparison with ocular irritation in vivo.

A study for the comparison of the action of tensio-active agents have been made with 3 models: The rabbit erythrocyte to evaluate the immediate membrane effect. SIRC fibroblasts (rabbit cornea) to evaluate the toxicity after 3 days. The rabbit eye (Draize). The cellular model have shown that there is a difference between non irritants tensio-active agents, and irritants-very irritants in vivo, except the BRIJ group. To evaluate the part of membrane effect in the interaction tensio-active-cell, it has been realized a test of haemolysis on the rabbit erythrocyte on 10 tensio-active agents. It shows that the cellular model can also determine a different toxicity than the one provoked by an aggression on the plasmic membrane.

Animals↗
PubMed1988

An extract of bovine thymus stimulates human keratinocyte growth in vitro.

An extract prepared from newborn calf thymus stimulated proliferation of human keratinocytes cultured from newborn foreskins and from skin biopsies of 26 adult volunteers aged 19 to 70 years. Growth over the 7-day assay period in the basal medium was age-dependent, with newborn cultures achieving a 10-fold increase in cell number over seeding density, old adult cultures barely maintaining their seeding density and young adult cultures intermediate in proliferative capacity. Maximally stimulatory extract concentration was 5-fold higher for newborn than for adult keratinocytes, with adult cultures experiencing toxicity at doses still growth-promoting for newborn cultures. At optimal extract concentration the maximal average increase in cell yield (66.3% for newborn, 53.6% for young adult, and 18.1% for old) indicated decreased mitogen responsiveness or increased inhibitor sensitivity with increasing donor age. Stimulation of cholera toxin-treated cultures was equally high, ranging from 39.4% to 145.9%, suggesting that the extract acts through a cyclic AMP-independent pathway. Thymic extract did not increase colony forming efficiency. Our findings provide further support for the concept of functional interactions between the skin and the immune system, in addition to the recognized morphologic similarities between thymic cells and keratinocytes. Furthermore, these data confirm earlier findings of an inverse relationship between mitogen responsiveness and donor age for cultured cells.

Adult↗
PubMed1985

Modulation of membrane receptor endocytosis by chemical effectors of membrane fluidity.

Several chemical effectors were used to induce changes in spleen B cell membrane fluidity. Membrane fluidity was monitored by fluorescence polarization analysis of the hydrophobic probe 1,6-diphenyl-1,3,5-hexatriene (DPH) and cell viability was checked not to be affected by the treatments. Membrane immunoglobulin (Ig) endocytosis by the living B cells with modified or unmodified membranes was quantitatively measured by flow cytometry, using a previously described method (Métézeau et al., 1982, 1984). The kinetics of endocytosis of membrane Ig was not affected by chemical effectors increasing membrane fluidity. On the contrary, increasing membrane microviscosity resulted in the slowing down and eventually the blocking of membrane Ig endocytosis. It is suggested that a step depending on membrane microviscosity is involved in the process of endocytosis; this step may become rate limiting when membranes are artificially rendered or naturally become (i.e. for pathological or particularly differentiated cells) more viscous.

Animals↗
PubMedDate not supplied

Human epidermis reconstructed on synthetic membrane: influence of experimental conditions on terminal differentiation.

Cell suspensions of human keratinocytes seeded onto cell culture inserts may undergo terminal differentiation in the absence of fibroblasts. Among the parameters that control these morphogenic events, exposure to air and the composition of the culture medium were investigated. In the latter case, three media were considered DMEM:Ham's F12, MCDB 153, and keratinocyte SFM medium at equivalent calcium (1.5 mM) and fetal calf serum (5%) concentrations. Immunochemical methods and transmission electron microscopy show that cells cultured in DMEM:Ham's F12 medium, and then raised at the air-liquid interface, form a basal layer plus suprabasal cell layers corresponding to the stratum spinosum, stratum granulosum, and stratum corneum. The suprabasal keratinocyte layers show morphologies that resemble intact skin in which cells are connected by desmosomes and contain intermediate filaments and keratohyalin-filaggrin granules. When the cultures are kept submerged, the keratinocytes show occasional keratohyalin granules and are connected by fewer desmosomes. Additionally, no proper stratum corneum is formed. In keratinocyte SFM medium and MCDB 153, cultures raised at the air-liquid interface are not able to form an epithelium of normal architecture and do not express terminal differentiation markers. Differentiation is initiated, however, since desmosomes and bundles of keratin filaments appear; on the other hand, filaggrin is not expressed even after 28 d in culture. Membrane-bound transglutaminase is expressed throughout the entire suprabasal compartment in MCDB153 and DMEM:Ham's F12 media but never appears in keratinocyte SFM medium. These studies show the relative independence of epidermal differentiation program to the composition (including the calcium concentration) of the media contacting the dermis and filling the extracellular space.(ABSTRACT TRUNCATED AT 250 WORDS)

Biomarkers↗