Biomedical subjects

A Meda

Publications and source records attributed to A Meda.

17 recordsLinked to original sources

PubMed2005

In ovo inhibition of fowlpoxvirus replication by a gall extract from Guiera senegalensis.

Several field isolates of fowlpoxvirus (FPV) from Burkina Faso, West Africa, were isolated and partly evaluated by molecular analysis. In addition, the in ovo antiviral activity against FPV of a gall extract from Guiera senegalensis was determined. Three viral isolates were obtained from suspected fowlpox cases after passage in embryonating chicken eggs and their poxviral identity confirmed by electron microscopy. All isolates were found to be pathogenic for chicks and all grew well in cell culture. Polymerase chain reaction and sequencing of amplicons revealed sequences identical with those of other FPV strains. The most studied isolate was then employed for use in an antiviral assay. An aqueous acetone extract from the galls of G. senegalensis was found to inhibit both virus-induced pock formation and to reduce viral titre in embryonating chicken eggs. The suggested mechanism of action is the activation of the alternative complement pathway and the inhibition of FPV-induced cholesterogenesis in ovo by constituents of the gall extract.

Animals↗

PubMed2005

The phytochemical composition and in vitro antiviral activity of decoctions from galls of Guiera senegalensis J.F. Gmel. (Combretaceae) and their relative non-toxicity for chickens.

Aqueous decoctions obtained from the galls of Guiera senegalensis were screened to determine their phytochemical composition and in vitro antiviral activity against fowlpox virus. In addition, we wanted to investigate the toxic effects, if any, of crude extracts in chickens. Steroids as well as cardiac glycosides not previously reported, an alkaloid, polyphenols and saponins were detected in the various fractions of organic solvents used for extracting the decoctions. Antiviral activity was determined by cytopathic effect inhibition assay in primary chicken embryo skin cells. The 50% inhibitory concentration (EC50) was shown to be 15.6 microg/ml. Toxicity for cells was established by determining the 50% cytotoxic concentration (CCy50). A value of 90 microg/ml and a selectivity index (CCy50/EC50) of 5.8 were obtained. In vivo studies of toxicity were performed in chickens that were dosed orally with decoctions of several concentrations for 2 weeks and then monitored for 3 months. No significant changes in several blood chemical parameters were obtained, except for a significant decline in SGOT levels in birds dosed with 100 mg/kg. These levels were nevertheless within the accepted normal range. The findings suggest that aqueous decoctions of galls from G. senegalensis are non-toxic for chickens when administered orally, even at a daily dose of 100 mg/kg for 14 days.

Administration, Oral↗

PubMed2003

[HIV in workplace: knowledges and attitudes of workers in three Senegaleses manufactures].

This study was conducted from may to august 2000 in three senegalese work sites randomly selected among seven which benefited from HIV preventive program. The aims were to evaluate knowledge, attitude and behavior related to HIV infection. A sample of 200 workers randomly chosen were asked about the ways of transmission and methods of protection as well as their risky attitudes and behaviors. Mean age was 41 years, 82.5% were male and 75.5% benefited from high school education; 75.75% had a good level of knowledge on HIV infection, 58% CI [51-65] stated having changed their sexual behavior into abstinence, fidelity to their partner (52.5%) and use of condom (46.5%). This good level of Knowledge and the positive changes in attitude are related to the high rate of alphabetization in our sample, information message diffused by media and preventive programs undertaken in workplace. Information campaigns via media should be maintained and strengthened and preventive programs diffused in all workplaces which will reduce diffusion of HIV in population.

Adult↗

PubMed1968