Search PubMed⌕ Search

Biomedical subjects

A Mariscal

Publications and source records attributed to A Mariscal.

At least 19 recordsLinked to original sources
PubMed2003

Is ilioinguinal/iliohypogastric nerve block always totally safe in children?

We report a case of accidental puncture of the small bowel during an ilioinguinal/iliohypogastric nerve block procedure for hernia repair. The diagnosis was made a few days later during a laparoscopic exploration owing to the progressive onset of clinical and radiological intestinal obstruction. A large, obstructing subserosal haematoma was found without any apparent perforation of the mucosa, and the damaged loop was resected. Such a complication has already been reported once in the literature after use of a long bevel needle for the puncture. This case is the first reported using an atraumatic short bevel needle. We discuss the technical aspects of the procedure and underline the fact that regional anaesthesia in children is never totally risk free.

Child↗
PubMed2002

Breast hemangioma mimicking carcinoma.

Breast hemangiomas are rare, and usually appear as well-delimited round or oval nodules at mammography. We report a case of a woman with a breast hemangioma, which mammographic features simulated malignancy, and present its pathologic correlation. Hemangiomas are benign vascular tumors that are rarely present in the breast, usually found incidentally on microscopy of biopsy material for other. They are occasionally detected by mammography, presenting as well-delimited round, lobulated nodules, sometimes with calcifications. We present the mammographic findings and pathologic correlation in a case of breast hemangioma with an atypical radiological manifestation, simulating a carcinoma. To our knowledge, this is the first reported case with these radiologic characteristics.

Journal Article↗
PubMed1999

Development and testing of a microbiological assay to detect residual effects of disinfectant on hard surfaces.

We describe a glucuronidase bioassay for detecting residual bactericidal activity from the use of disinfectants on hard surfaces; in this assay we used formaldehyde, ethanol, isopropanol, chlorine, and a commercial preparation containing 2-bromo-2-nitro-1, 3-propanediol. Chlorine and the commercial preparation showed bactericidal activity (53.5% and 98.2%, respectively) for a week after disinfection.

2-Propanol↗
PubMed1998

Interference by carbohydrate substrates, flavonoids, and monosaccharide derivatives on bacterial beta-D-glucuronidase assays.

Most commercially available test kits for water and foodstuffs use beta-galactosidase activity for coliforms and beta-glucuronidase activity for Escherichia coli. We tested the effects on the beta-glucuronidase activity of E. coli W3110 of substances usually present in foods and several synthetic pharmaceutical compounds. Thirteen substances were tested: three carbohydrates, four flavonoids, five monosaccharide derivatives, and dimethyl sulphoxide. In a minimum medium without any other carbon source, glucose (0.1 mM), quercetin (0.1 mM), silymarin (10 mg/L), D-gluconic acid (0.01 mM), D-gluconic acid lactone (0.01 mM), isopropyl-beta-D-thiogalacto pyranoside (1 mM), p-nitrophenyl beta-D-glucuronide (1 mM), and DMSO (1 M) completely inhibited E. coli glucuronidase activity at the above concentrations. However, the following compounds stimulated E. coli glucuronidase activity within the ranges of concentrations shown: glucose (0.0001-0.01 mM), lactose and sucrose (>0.1 mM), D-saccharic acid 1,4 lactone (0.0001-0.1 mM), p-nitrophenyl beta-D-glucuronide (0.001-0.01 mM) and DMSO (2-500 mM). In a rich culture medium that contained other carbon sources (lauryl tryptose broth) E. coli glucuronidase activity in the presence of the extra nutrients was unaffected by the test substances and therefore, under normal conditions in water or foods, they should not interfere with E. coli assays based on measurements of beta-glucuronidase activity.

Biological Assay↗
PubMed1997

A trial to compare the effects of pH, buffer concentration, and NaCl, on one fluorescent and two bioluminescent bacterial tests for acute toxicity.

Of all the bioassays to determine acute toxicity described in the literature, those that employ bacteria as indicator organisms are usually the most rapid and the most economic, although alone they cannot predict the possible toxic effect of any type of substance. When bioassays are employed to test the toxicity of known substances and of compounds in samples from waste discharges they have to work in very different conditions from those for which they are designed. The effects of three factors, pH, buffer concentration, and NaCl, on the performance of a fluorogenic bioassay based on the beta-glucuronidase activity of Escherichia coli were investigated. The results of this test were compared with those of two known biluminescent bacteria tests. The fluorogenic bioassay has a more restricted optimum pH range, while the influence of buffer concentration was similar for the three tests. E. coli glucuronidase activity was affected at a concentration as low as 128 mg/l of NaCl. Changes in the pH or buffer concentrations or chloride ions, greatly influenced the respectives toxicities of four substances, acridine orange, TEMED, 2-mercaptoethanol, and mercuric chloride.

Acridine Orange↗
PubMed1994

New toxicity determination method that uses fluorescent assay of Escherichia coli.

We describe a new method that uses a fluorogenic bioassay of the beta-glucuronidase conversion of 4-methylumbelliferyl beta-D-glucuronide (MUG) to 4-methylumbelliferone to evaluate the individual toxic effects on Escherichia coli of Al3+, Cr6+, Hg2+ and Li+. This work was designed to examine the effectiveness of this method to measure the effects of five ionic concentrations of either Al3+, Cr6+, Hg2+ or Li+, on the growth of E. coli in a minimal medium that had MUG as the only source of carbon. This method was simple and fast, and its toxicity detection sensitivity was equal to, or greater than, existing bacterial bioassays. The use of the MUG substrate minimized the danger of interference by bacteria other than E. coli. Evaluations of toxicity in samples of public drinking water proved equally sensitive.

Aluminum↗
PubMed1992

Juvenile xanthogranuloma with extracutaneous lesions.

The purpose of this article is to present a case of juvenile xanthogranuloma with pulmonary, retroperitoneal and splenic involvement. The similar echographic pattern of the cutaneous and visceral lesions was very helpful in order to suggest the correct diagnosis.

Abdomen↗
PubMed1991

[Cerebral tuberculoma. Report of 8 cases].

A series of eight patients with cerebral tuberculoma seen between 1980 and 1990 is reported. Headache was the leading clinical finding, followed by focal neurological findings and seizures. Papilledema was the most uniform examination finding associated with intracranial hypertension. Five patients had another localization of tuberculosis: three had tuberculous meningitis, one had tuberculous pericarditis and only one had associated active pulmonary involvement. Cerebrospinal fluid was investigated in seven patients. In three patients data of tuberculous meningitis were found, with positive culture in Löwenstein medium. Two patients had the acquired immunodeficiency syndrome. All patients received medical treatment with good response, except in one who developed paradoxical expansion of the lesions after two months of therapy.

Adult↗
PubMed1991

Effect of penicillin and chloramphenicol on the growth and endotoxin release by N. meningitidis.

The action of two antibiotics, penicillin and chloramphenicol, on bacterial growth and endotoxin liberation was studied in 18 strains of Neissevia meningitidis isolated from blood and CSF of patients with meningococcal infections. The antibiotics were administered both separately and in combined form in doses equivalent to 1 MIC and 100 MIC. Penicillin was found to produce a faster and more intense bactericidal effect than chloramphenicol during the first hour, whereas at 12 h these differences were not significant. This could explain the initial worsening observed in some infected patients when large doses of penicillin are administered. An increased liberation of endotoxin after adding penicillin was observed in six of the strains studied, whereas the remaining 12 did not show significant increases. The six strains (belonging to serogroup B) were known to have an enhanced capacity for spontaneous endotoxin liberation.

Chloramphenicol↗
PubMed1989

The effect of 2,4-dichlorophenol on growth and plasmidic beta-lactamase activity in Escherichia coli.

The toxic effects of 2,4-dichlorophenol on two strains of Escherichia coli harbouring plasmids with beta-lactamase activity were studied. The toxicant had a significant effect on growth at concentrations of 12 micrograms ml-1 for the JA 221 (pBR322) strain, as well as at 24 micrograms ml-1 for the J5-3 (RP4) strain. beta-Lactamase production was affected at concentrations of 96 micrograms ml-1 in both strains. However, beta-lactamase production does not seem to constitute a reliable toxicity test in view of its variability and relatively low sensitivity.

Chlorophenols↗