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Biomedical subjects

A Mariano

Publications and source records attributed to A Mariano.

76 records · Page 5Linked to original sources

[Production, purification and characterization of some Clostridium perfringens toxins].

Cultures of Clostridium perfringens produce a factor which hydrolyzes phospholipids. In this paper we report the separation and the purification from the growth medium of Clostridium perfringens of two phospholipase activities: a specific sphingomyelinase, and a specific lecithinase. Both the phospholipase activities have been purified 300-fold by fractionation of the growth medium with ammonium sulfate, filtration on Sephadex G-100 and chromatography on DEAE cellulose. The sphingomyelinase activity is completely inhibited by 10(-3) M calcium chloride, hydrolyzed lysolecithin and dipalmitoyllecithin at about 10% of the rate of sphingomyelin. No hydrolysis of phosphatidylserine, phosphatidylethanolamine or phosphatidylinositol is detected. From the same growth medium a theta-toxin activity was also purified.

Bacterial Toxins↗

Occupational asthma due to colophony in non-industrial environments.

Colophony is largely used in industrial environments, like the electronics and the rubber tyre industries, but it is also present in non-industrial environments as a constituent of glues and paper. For this reason it is one of the commonest skin sensitizers. Many cases of occupational asthma due to colophony have been described, but reports apart from electronic industry are not common. The case is described of a subject employed in administrative work who developed asthma due to colophony from using sealing wax to seal samples of food packages.

Asthma↗