Acrosin and the outer acrosomal membrane (OAM) were studied in the spermatozoa of 9 infertile patients who differed in the number of round-headed spermatozoa between 14 and 71% in their ejaculates. These sperm components were also investigated in two infertile brothers who exhibited exclusively round-headed spermatozoa in their ejaculates, and in their fertile father. It turned out that round-headed spermatozoa lack both acrosin and the OAM as studied by indirect immunofluorescent and immunoperoxidase staining technique, gelatinolysis tests and by acrosin activity measurements. The normally shaped spermatozoa of 6 of the 9 infertile patients were found to be positive for acrosin and the OAM as expected, but in the remaining three patients even these spermatozoa were abnormal; in one patient they were unstainable for acrosin and in two patients they were unstainable both for acrosin and the OAM. These results have been confirmed by studies with the gelatinolysis test. The father of the two brothers with exclusively acrosomeless spermatozoa had more than 94% of normally shaped spermatozoa. However, only 10% of these spermatozoa were acrosin positive and only 30% were positive for the OAM. On the basis of these results we postulate that the mode of inheritance of the round-headed spermatozoa syndrome is polygenic rather than monogenic as suggested by previous authors.

Acrosin↗

PubMedDate not supplied

On the interaction of bull and boar acrosins with the zona pellucida of different mammalian species in vitro.

Acrosin was prepared from boar and bull spermatozoa and its lytic effect in vitro on the zona pellucida of mouse, golden hamster, rabbit, pig and cow was investigated. Depending upon the species studied, ovarian oocytes, ovulated oocytes and preimplantation embryos were obtained for the experiments. While in golden hamster and rabbit the zona pellucida was removed by both acrosins, this effect was absent for bull acrosin in cow and mouse eggs and for boar acrosin in pig and mouse eggs. In those species in which the zona pellucida was not removed by the acrosins after an incubation period of 2 hours even a prolongation up to 24 hours with higher amounts of acrosin, the addition of acrosomal extracts to the incubation buffer (Tyrode solution pH 7.2) or an increase of the pH value up to 8.6 of the acrosin solution had no effect upon the zona pellucida. Our results indicate that at least in vitro, acrosin does not possess the capacity to lyse the zona pellucida in a species specific fashion. Since the lytic effect of boar and bull acrosin on the zona pellucida of ovarian oocytes and preimplantation embryos is not different from that on ovulated oocytes it can be assumed that neither the maturation of the zona pellucida during oogenesis nor its modification after fertilization, change the susceptibility of the zona pellucida to acrosin digestion.

Acrosin↗

PubMedDate not supplied

[Surgical treatment of primary hyperparathyroidism. Apropos of 11 cases].

Over a period of 10 years, 11 cases of primary hyperparathyroidism were operated on and identified as 7 adenomas and 4 hyperplasias. One post-operative death was noted in a 80 year woman with advanced disease. Three hypocalcemias and one temporary unilateral recurrent nerve plasy were observed. After the results of this series, various clinical forms were identified and surgical modalities were altered according to the intra operative findings.

Adenoma↗

PubMedDate not supplied

[Residual lithiasis of the common bile duct. Surgical or endoscopic treatment?].

The authors report their experience concerning residual lithiasis of the common bile duct, while trying to define the respective place of surgery and endoscopy in this condition. There is a clear female predominance in their series, the mean age being 41 years. The indications for surgery and endoscopy are discussed and the means of prevention are reviewed.

Gallstones↗

PubMedDate not supplied

[Differentiated cancer of the thyroid. Prognostic factors and therapeutic attitude].

Differentiated cancer of the thyroid. Prognostic factors and therapeutic attitude. This study deals with a series of 43 cases of differentiated cancer of the thyroid operated in our unit of IBN ROCHD University Hospital of Casablanca between 1981 and 1985. Our purpose is to expose our therapeutic philosophy and to compare our results with those of the literature. The youngest patient is 16 years old, the oldest is 70. The averaged aged is 43 years, 84% of the patients are females and only 16% are male. Papillary cancer constitutes the main group with 62%, the vesicular carcinoma represents only 38%. Our treatment management is made of surgery associated or not to radioactive iodine, but systematically followed by permanent hormonotherapy. Prognostic factors are reported and discussed. Actually three different surgical attitudes are confronted: the maximalistic, the minimalistic and the eclectic ones. Regular and long term follow-up of operated patients is considered of importance.

Adolescent↗

PubMedDate not supplied

[Hydatid cyst of the pancreas. Apropos of 2 case reports].

Hydatic cyst of pancreas is a rare disease, the symptomatology is polymorph and the diagnosis is rarely evocated before surgery. Epidemiological, clinical, biological (immunofluorescence) and echographic data should allow its better detection. Surgical treatment is function of the cyst localisation: exeresis for left lesions and conservative surgery for right lesions.

Abdominal Neoplasms↗

PubMedDate not supplied

[Results of conservative surgery of the bulging dome in hydatid cyst of the liver (apropos of 183 cases)].

The authors report the short interval results of a series of 183 patients operated on for hydatic cyst of the liver; all had undergone "the conservative operation" of resection of the bulging dome. Mortality and morbidity rates are similar to those of recently published studies on radical treatment. The benignity and the results explain the value of this routine method of treatment of hydatic cyst of the liver.

Adolescent↗

PubMedDate not supplied

[Angioterebrant duodenal ulcer. Place of ligation of the gastroduodenal artery in recurrent hemorrhages].

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Adult↗

PubMedDate not supplied

Osteomalacia as a presenting manifestation of Sjögren's syndrome.

Osteomalacia is still common in Morocco, where the leading causes are nutritional deficiencies followed by intestinal diseases. Osteomalacia rarely occurs as the first manifestation of a renal tubule disorder due to a connective tissue disease such as Sjögren's syndrome. The case of a 40-year-old woman who presented with a five-year history of generalized bone pain, severe weight loss and a waddling gait is reported. She had low levels of serum phosphate (0.74 mmol/L), serum calcium (1.97 mmol/L), and urinary calcium (1.22 mmol/24 h). Serum alkaline phosphatase was 210 IU/L. Roentgenograms showed Looser's zones (right femoral neck, sixth and seventh right ribs). There was bilateral parotid gland enlargement, dryness of the mouth, nose and eyes, and bilateral punctate keratitis. A lip biopsy showed changes corresponding to stage II of the Chisholm and Mason classification. Tests for rheumatoid factor (latex and Waaler-Rose) and antinuclear factor were negative. The alkaline reserve was 18 mmol/L, serum potassium was 3.5 mmol/L, serum chloride was 112 mmol/L and urinary pH was 6.5. A renal biopsy showed tubulointerstitial lesions, lymphoplasmocytic infiltrates and interstitial sclerosis with patchy tubular atrophy. The patient was given bicarbonates, high-dose vitamin D followed by 1-alpha-hydroxycholecalciferol (0.3 microgram/d), and calcium (1 g/d). Follow-up was 42 months at the time of this writing. The role of tubular disorders in the genesis of osteomalacia is discussed, and the renal manifestations of Sjögren's syndrome are reviewed.

Acidosis, Renal Tubular↗