[Acute HIV disease].
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Biomedical subjects
Publications and source records attributed to A Maeland.
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3 patients with salmonella infections who continued to excrete salmonella in fecal samples for more than 6 months were treated with pivmecillinam alone or in combination with pivampicillin. Mecillinam alone cured 1 of the carriers, but was without success in the 2 others. One of these patients was cured after treatment with pivmecillinam and pivampicillin. In the last patient salmonella excretion ceased after treatment with pivmecillinam, pivampicillin and probenecid. The choice of treatment was based upon in vitro demonstration of synergism between mecillinam and ampicillin. In the last patient the final treatment was chosen after studies of the bactericidal effect of the patient's serum during various regimes.
The aim of this study was to elucidate the mechanisms by which sucrose improves growth in a hypertonic medium for isolating aerobes from blood. Clinical blood cultures were made routinely in duplicate in plain broth consisting of brain heart infusion broth with sodium polyanetholesulfonate, gelatin, and penicillinase and the same broth with 20% sucrose added. The growth patterns of Staphylococcus aureus and Enterobacteriaceae from plain and from hypertonic broth were correlated with the presence or absence of antimicrobial therapy in patients when the blood cultures were collected. In S. aureus bacteremias, 58.7% of the positive cultures collected during treatment of patients with beta-lactam antibiotics showed earlier growth or growth only in hypertonic broth, compared with 16.7% of the cultures taken during treatment with other antimicrobial agents (P less than 0.05) and 17.6% of the cultures made in antibiotic-free intervals (P less than 0.01). In the group of cultures yielding growth of Enterobacteriaceae, growth occurred earlier or solely in hypertonic broth in 28.9% of the cultures taken during treatment with beta-lactam antibiotics, compared with 15.7% of the cultures taken during treatment with other antimicrobial agents and 21.6% of the cultures collected in antibiotic-free intervals (differences not statistically significant). It is concluded that treatment with beta-lactam antibiotics is an important reason for the improved growth of S. aureus from hypertonic broth, but other factors are also involved.
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