Search PubMed⌕ Search

Biomedical subjects

A M Wilson

Publications and source records attributed to A M Wilson.

At least 163 records · Page 9Linked to original sources

Amylase synthesis and stability in crested wheatgrass seeds at low water potentials.

Drying of seeds of Agropyron desertorum (Fisch. ex Link) Schult. did not result in breakdown of alpha-amylase nor impair the ability of seeds to resume its synthesis when moistened again. beta-Amylase activity did not change during 5 days of germination at a water potential of 0 atmosphere nor during 40 days of incubation at -40 atmospheres. Seeds synthesized alpha-amylase at 0, -20, and -40 atmospheres, but not at -60 atmospheres. At 0 and -20 atmospheres, the log of alpha-amylase activity was linearly related to hastening of germination. But at -40 atmospheres, seeds synthesized alpha-amylase during a time when there was little hastening of germination. Thus, it appears that other biochemical reactions are less drought-tolerant than synthesis of alpha-amylase. It is concluded that inhibition of alpha-amylase synthesis is not a controlling factor in the germination of these seeds at low water potentials.

Journal Article↗

Phosphorylation in crested wheatgrass seeds at low water potentials.

Crested wheatgrass seeds [Agropyron desertorum (Fisch. ex Link) Schult.] were tested for their ability to carry on phosphorylation reactions at low water potentials. Seeds were treated with (32)P labeled sodium phosphate and incubated in air having different controlled relative humidities. Ion exchange chromatography and radioassay of phosphate esters indicated that some phosphorylation occurred at a water potential of -880 atmospheres. Seeds did not incorporate (32)P in nicotinamide adenine dinucleotide, adenosine triphosphate, and uridine diphosphate hexose until they were moistened to a water potential of -130 atmospheres.

Journal Article↗