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Biomedical subjects

A M Roch

Publications and source records attributed to A M Roch.

25 records · Page 2Linked to original sources
PubMed1980

[Immunochemical evidence for the specific binding of putrescine to plasma proteins including fibronectin].

Human plasma contains proteins capable of binding 14C putrescine by the action of Ca++ activated transglutaminase. These proteins have molecular weights from 32 to 220 K and above. One of these (with a molecular weight of 220 K) has been identified as fibronectin by the use of an antifibronectin antiserum. Evidence for a protein with a molecular weight identical to that of fibronectin has been obtained on PAGE analysis of the precipitate formed on incubating human serum with antipolyamine antiserum.

Calcium↗
PubMed1979

Evidence for natural antibodies (IgG) to polyamines in human sera.

Human sera contain IgGs that react with latex-putrescine spheres. Identification of the IgGs has been achieved by polyacrylamide gel electrophoresis and their reaction with peroxidase-labeled antihuman gamma-chain-specific antibodies. The inhibition of IgG fixation to latex-putrescine spheres by free spermine and putrescine provides evidence that these IgGs are specific for polyamines.

Antibody Specificity↗
PubMed1978

The preparation of latex particles with covalently bound polyamines, IgG and measles agglutinins and their use in visual agglutination tests.

Carboxylated latex particles were substituted with side arms terminating in primary amine and hydrazine groups. The particles were coupled to aldehyde groups generated on glycoproteins which were treated with sodium periodate. Particles having the alipathic primary amine putrescine hapten as the sole substituent and particles linked to glycoproteins such as measles agglutinins and IgG were used to detect the presence of the corresponding antibodies or antigens in biological fluids by agglutination tests.

Agglutination Tests↗
PubMed1977

Nephelometric assay of antigens and antibodies with latex particles.

Antigens and antibodies covalently bound to latex particles have been used to detect specifically corresponding antibodies and antigens by nephelometry. A systematic study of factors such as wavelength, angle of observation, concentration of latex particles, and reaction time has permitted us to develop a procedure suitable for routine estimation of antigens and antibodies in the nano and picogramme range.

Antibodies↗