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Biomedical subjects

A M Ramos

Publications and source records attributed to A M Ramos.

14 recordsLinked to original sources
PubMed2000

Qualitative and quantitative analysis of phosphorylated compounds in milk by means of 31P-NMR.

31P-NMR has been tested as a technique for determining the phosphorus content of milk samples in both the inorganic form (Pi) and bonded to caseins as phosphoserine (SerP). 31P-NMR made possible the simultaneous determination of Pi and SerP in approximately 1.7 h. The determination is based on measuring the area under the resonance in question and interpolating in a calibration curve prepared using external standards of known phosphorus contents and prepared in the same manner. The concentration area plots obtained were linear in the ranges 162-1993 mg P/l for Pi and 38-40.2 mg P/l for casein SerP. The repeatability of the method was good. When the same milk samples were analysed by both 31P-NMR and the classic colorimetric method the correlation was good. Polyphosphates added to commercial milk were also detected, as diphosphates. The NMR response for diphosphate was linear between 58 and 576 mg P/l and preliminary results indicated that quantitative analysis could be achieved in commercial milk.

Animals↗
PubMed1999

[Nicotine dependence: 1-year follow-up patients treated with nicotine replacement and group therapy].

BACKGROUND: Smoking is the main single avoidable cause of death in our country. Little research in the treatment of such disorder has been made. AIM: To report the results of a prospective follow up for one year of outpatients from our "Smoker's Clinic" at the Department of Psychiatry of the Catholic University. PATIENTS AND METHODS: One hundred twenty-seven patients (84 male, aged 21 to 70 years old), with DSM-IV criteria for nicotine dependence, were included in a total of 18 groups. Each group received an intensive treatment program of 10 sessions with cognitive-behavioral relapse prevention techniques and nicotine replacement. Patients with active psychiatric diseases were not included in the program. RESULTS: Eighty-seven percent of subjects were abstinent at the first month and 50% were still abstinent after twelve months of follow up. We did not find differences in gender, age, previous psychiatric disease, number of cigarettes and breath carbon monoxide level between abstinent and non abstinent patients after 12 months of follow up. CONCLUSION: This intensive nicotine dependence treatment in seriously dependent patients, proved to be successful, regardless of the previous psychiatric history.

Adult↗
PubMed1995

A simple method for detecting subcutaneous phaeohyphomycosis with light-colored fungi. A study of eight cases.

Eight cases of subcutaneous phaeohyphomycosis are presented. A lack or scarcity of fungal pigmentation was seen in six of these cases. The simple act of lowering the microscope condenser makes these uncolored fungi easily visible. The thick hyaline capsule becomes refringent, presenting single oval or round cells and septated hyphae, which can be short or elongated, branched or unbranched, and irregularly swollen. The dematiacious character of the fungi is confirmed by using the Fontana-Masson stain. The fungi are not visible under polarized light. Our studies point to the probability of subcutaneous phaeohyphomycosis being a widespread tropical disease in Brazil. However, it is rarely diagnosed because of the common practice of fixing tissue specimens without prior culturing and the difficulties of detecting the fungal forms using customary microscopic procedures, especially when the fungi have a lack of pigment and are hard to detect by hematoxylin and eosin stain.

Adult↗
PubMed1991

Detection of antigens in urine of patients with acute falciparum and vivax malaria infections.

Using an antigen-capture, dot-blot assay, antigens were detected in the urine of 50 patients infected with Plasmodium falciparum. Antigens were also detected in 12/15 patients who had no detectable parasitemias 1-2 weeks after chemotherapy. By Western blotting and immunoprecipitation, four predominant antigens were identified with the following molecular masses (Mr) and isoelectric points (pI): antigen 1, 200 kDa, pI 6.4-6.27; antigen 2, 180 kDa, pI 5.2-4.8; antigen 3, 150 kDa, pI 5.5; antigen 4, 96 kDa, pI 5.1-4.8. These antigens were heat stable to 100 degrees C for 5 min. Antigens were also detected in the urine of 35 patients with acute P. vivax infections by Western blotting and dot-blot analysis and 10/10 patients three weeks following chemotherapy. The antigens had Mr of 200, 170, and 130 kDa.

Acute Disease↗
PubMedDate not supplied

Project Pró-natal: population-based study of perinatal and infant mortality in natal, Northeast Brazil.

The Pró-Natal project is a collaborative initiative that aims to improve maternal and infant health in a deprived community in Natal, Northeast Brazil. To assess the perinatal and infant mortality in this population of 40,000, we have collected over a 2-year period a consecutive series of 39 autopsy examinations on deaths under 1 year of age. During this period there were 2212 live births in the study population. The 14 perinatal deaths are described using the Wrigglesworth classification, and the 25 infant deaths, using a clinicopathological system. The contribution of normally formed stillbirths was small (14%), which probably reflects the underreporting of stillbirths in this community. The most common cause of death in the live births was complications of prematurity (43%). Specific causes (22%) of perinatal deaths were predominantly infections, including one case of congenital syphilis. Perinatal asphyxia was diagnosed in 14%, and there was one case (7%) of a chromosome abnormality. Infant deaths were predominantly due to respiratory (45%) and gastrointestinal infections (28%), with chronic malnutrition as an underlying cause in 80% of cases. Prenatal care could theoretically have prevented three of the perinatal deaths, and a further six deaths could have been avoided by improved management of labor and the immediate neonatal period. Prevention of malnutrition and improved treatment of acute infections would contribute to a reduction in infant mortality in this population. The Pró-Natal project will use these data to design preventative interventions to reduce perinatal and infant mortality in this community.

Autopsy↗
PubMedDate not supplied

[Fatal visceral leishmaniasis associated with acquired immunodeficiency syndrome: report of a case with necropsy findings and immunohistochemical study].

A case of fatal visceral leishmaniasis associated with immunodeficiency syndrome in a 32 year-old male patient is reported. The protozoonosis was responsible for the patient's death. Visceral leishmaniasis showed itself in an atypical form, at necropsy, with an intense parasitation of the mononuclear phagocitic system and damaging organs not usually affected by the disease, such as the adrenals, the kidneys, the lungs and the brain. Parasitised cells were observed within small vessels in several tissues. An immunohistochemical study was done on samples from the spleen, lymph nodes and brain, showing strong reactivity with antibody directed against leishmania.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Control of blood transfusion malaria in an endemic and in a non-endemic region in Brazil].

The aim of the present work was to establish appropriate criteria for screening of donor blood from regions with distinct Malaria epidemiological characteristics. Three locations with different screening criteria were studied: São Paulo, SP (with no vectorial transmission), Belém, PA (with low active transmission) and Matupá and Peixoto de Azevedo, MT (with high active transmission). The Malaria parasite--Plasmodium sp--was searched for by "thick film", QBC Test and antigen Immunofluorescence test, and was not detected in any of the samples. There was, however, a great variation in the positivity of anti-plasmodial antibodies, as determined by IIF-IgG anti-P. vivax and -P. falciparum, between accepted donors in the 3 studied locations and between rejected and accepted donors in São Paulo (1.98% accepted, 22.3% rejected--p < 0.01) and Belém (17.2% accepted, 58.3% rejected--p < 0.01). These data endorse the use of the applied clinical and epidemiological screening. In Matupá and Peixoto de Azevedo, where there was no rejected donor, the serological positivity was 80.6%. We, therefore, consider that the Malaria screening in blood banks should follow clinical and epidemiological criteria suitable to each region. The laboratorial screening techniques should then detect either the parasites (thick film/QBC Test or the parasite antigens.

Blood Donors↗
PubMedDate not supplied

[Celiac disease: report of 20 cases in Rio Grande do Norte, Brazil].

This paper shows that celiac disease exists in Northeast of Brazil and can be one of the causes of malnutrition in the State of Rio Grande do Norte. The authors present a study of 20 cases of celiac disease in the State of Rio Grande do Norte where there had not been any cases diagnosed before 1978. Ethnic aspects in the State seem to have influenced the occurrence of the disease since the European miscigenation has been more frequent than that of negroes and local indians. The cases are analysed in this paper and correlated with the literature.

Body Height↗
PubMedDate not supplied

[Endoglucanase production in 4 species of the genus Saccobolus].

Endoglucanase production was measured in culture filtrates of four species of Saccobolus growing in media containing glucose or crystalline cellulose as the only carbon sources. Enzyme activity was four to seven times higher in the presence of cellulose than glucose. S. saccoboloides showed maximal growth and enzyme production. The extracellular proteins secreted during growth on cellulose were separated by polyacrylamide gel electrophoresis and stained for proteins. A zymogram technique was used to visualize bands with endoglucanase activity. The four species showed different protein and isoenzyme patterns.

Ascomycota↗
PubMedDate not supplied

[Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)].

Growth and beta-1, 4 endoglucanase production of six species of Saccobolus were determined. Twenty nine strains of thirteen geographical isolates were tested, using culture medium with crystalline cellulose as carbon source. All species showed good growth and enzyme activity. A high intraspecific homogeneity was observed as there were no differences in the variables measured within the strains of each geographical isolate and the only difference was between the two isolates of S. infestans. Each species had a characteristic electromorph, showing a variable number of isozymes with a maximum of 11 for S. seudodepauperatus and a minimum of 7 for S. platensis. These results support the usage of isoenzymatic patterns of beta-1, 4 endoglucanase and physiological parameters for the identification of species of Saccobolus as an aid to traditional taxonomy. This methodology is an additional tool to traditional taxonomy, based only on morphological characters.

Ascomycota↗