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Biomedical subjects

A M Murphy

Publications and source records attributed to A M Murphy.

At least 109 records · Page 6Linked to original sources
PubMed1972

Differential immune response to attenuated rubella virus vaccine.

Serum samples collected from 18 volunteers 1, 2, and 3 months after vaccination with Cendehill strain of attenuated rubella virus vaccine were fractionated on a Sephadex G-200 column to test for immunoglobulin M (IgM) antibody. Seventeen of the 18 had demonstrable IgM antibody in serum collected 1 month postvaccination whereas only 5 and 3 had IgM antibody 2 and 3 months, respectively, post-inoculation. The geometric mean titers of hemagglutination-inhibiting antibody in the 19S fraction were 38, 7, and 6 at 1, 2, and 3 months after vaccination, whereas the corresponding 7S titers were 29, 53, and 57, respectively. Four other seronegative volunteers vaccinated with Cendevax were tested serially for total antibody only in whole blood obtained by finger pricks. Three of them showed appearance of antibody between 14 and 17 days and one between 17 and 21 days. It appears that vaccine-induced immune response is similar to natural infection especially with regard to the time of appearance of antibody and the relative proportions of IgM and IgG antibody thus produced.

Animals↗
PubMed1971

Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column.

To facilitate the diagnosis of recent rubella infection, rubella haemagglutination inhibiting antibody has been determined in four fractions obtained by Sephadex G-200 gel filtration of samples of serum. All the 21 samples collected at the convalescent stage of the disease had varying proportions of haemagglutination inhibiting antibody in fraction 1, representing the major portion of IgM antibody whereas all but three out of 22 sera from persons with no history of recent rubella had negative titres in this fraction. The haemagglutination inhibiting titres in the three positive sera in the second group was very low as compared to the other fractions. Fractionation of sera on a Sephadex G-200 column coupled with the rubella haemagglutination inhibition test can, therefore, be used to diagnose recent rubella infection.

Adult↗