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Biomedical subjects

A M Markslag

Publications and source records attributed to A M Markslag.

4 recordsLinked to original sources

Mechanical loading on the low back in three methods of refuse collecting.

The mechanical loading on the low back was studied in three different current methods of refuse collecting: in polythene bags, two-wheeled mini-containers and large four-wheeled containers. To this end the most prominent activities of each collecting method were performed in a laboratory. On the basis of movement analysis, force measurements and biomechanical modelling, spinal compressive and shear forces were estimated. From these forces and from the frequency of activities during the working day (assessed in a preliminary field study) the low-back stress in each collecting method was evaluated. In the bag-method, peak forces when throwing the bags ranged from 3341 to 5179 N (average compression) and from 284 to 673 N (shear) among the different conditions studied. The act of picking up bags also showed rather high forces (exceeding the NIOSH limit for disc compression in most cases). The frequency of exposure to these forces in the field is rather high (workers pick up and throw on average 807 times each day). The mini-container method compares favourably to the bags method. Peak compressive and shear force in tilting/pushing and pulling mini-containers ranged from 1657 to 2654 N and from 123 to 248 N respectively. Also, the frequency of stressful events in the field is lower in this method. In the large container method extremely high peak forces (e.g. compression ranged from 4991 to 5810 N) were observed in the task of putting the empty container back from street level to sidewalk level (surmounting the kerb). The frequency of activities like pushing, pulling and lifting the large container in the field is much lower compared with activities in the other methods. On the basis of the frequency and magnitude of spinal forces it was concluded that the mini-containers should be preferred to the bags. If kerbs are removed at container places and tasks are performed by two instead of a single person, the large container method would form another good alternative to the stressful task of collecting refuse in bags.

Adult↗

Human hair follicles may be used for population screening of heterozygotes of glucose-6-phosphate dehydrogenase deficiency.

The carrier status for glucose-6-phosphate dehydrogenase deficiency can still be detected with the use of hair follicles after a period up to 10-14 days after plucking, even under conditions of temperature and humidity that are encountered in tropical countries, where the disease is most common. Furthermore, it is shown that the hair colour or age of the donor has no influence on the enzyme activity. As a consequence, the hair follicle is indeed suitable for screening in rural areas since mailing to existing medical centres is possible.

Adult↗

A simple fluorimetric microassay for DNA in hair follicles or fractions of hair follicles.

Human hair follicles may be useful for determining individual differences in the metabolism of polycyclic aromatic hydrocarbons in epithelial tissues. for quantitative measurements of carcinogen metabolism, determination of the amount of DNA in the hair follicles is necessary in order to correct for individual size variation. A simple method for DNa determination in hair follicles is described, based on the use of a hypotonic pronase solution to solubilise DNA which is then available for complex formation with mithramycin or 4,6-diamidino-2-phenylindole. 2HCI (DAPI). The mithramycin method permits the measurement of DNA in a small number of hair follicles, while the DAPI method is sensitive for as little as one single bulb.

DNA↗

Urinary oxalate estimation.

A method of estimating urinary oxalate is described. Impurities which might otherwise interfere with the estimation are removed by means of an ion-exchange resin. The oxalate is then reduced to glycolic acid, which forms a coloured compound with chromotropic acid. It can then be estimated colorimetrically. No special equipment is required, oxalate recovery is good, and the method is reproducible and precise.

Chromatography, Ion Exchange↗