Biomedical subjects

A M Mangoud

Publications and source records attributed to A M Mangoud.

At least 37 records · Page 2Linked to original sources

PubMed1992

Histo- and immuno-pathological and electron microscopy studies of the splenic red pulps of schistosomiasis mansoni patients.

The histo- and immuno-pathological and electron microscopy (EM.) of the splenic red pulps studies were carried out for schistosomiasis mansoni patients and normal controls. The main pathological processes were interstitial haemorrhages, congestion, proliferation and hyperplasia of the red pulps of the schistosomiasis mansoni patients. The IgG showed marked reaction, moderate with IgA and mild with IgM. The E.M. showed an increase in the size of the different types of the reticular cells particularly those of the nuclear membrane with type II, nuclear degeneration, deposition of fibrin threads, marked fibrosis and phagocytosis and formation of pores at the sinusoidal basement membrane. It was concluded that these pathological processes and the alteration in the immunoregulatory system of the spleen of schistosomiasis mansoni patients reflect the role played and perpetrated in the development of splenomegaly.

Humans↗

PubMed1992

Myopathic changes in schistosomal hepatosplenomegaly; histopathological, histochemical and immunopathological studies.

Eighteen patients suffering mainly from schistosomal hepatosplenomegaly (B.H.S.M.) were selected & classified into 3 groups according to the degree of B.H.S.M. Muscle biopsy for histopathological, histochemical and immunopathological studies were taken. The results showed that fatiguability as a symptom was present in all cases but motor weakness was present in four cases. Twelve cases had peripheral neuropathy in the form of glove and stocking hypothesia, while pyramidal tract lesion was present in four cases. Two cases, however, showed both peripheral neuropathy and pyramidal tract lesions. Histopathological and histochemical assessment revealed frank myopathic changes in 14 cases while only one case showed definite neuropathic changes. The remainder cases were inconclusive. Immunopathological examination revealed marked deposition of IgG in 50% of cases, while IgM and IgA showed mild to moderate reactions. It was concluded that muscular changes in schistosomal patients are myopathic in nature and that immunological mechanisms could be considered as a factor in the pathogenesis of this muscular disorder.

Adolescent↗

PubMed1992

Lymphatic leishmaniasis. An adult case without visceral involvement from Sharkia Governorate, Egypt.

Lymphatic leishmaniasis was reported as an indigenous infection in a 30 year old male from Abo Hamad, Sharkia, Egypt. Cervical lymphadenopathy was the only clinical sign and no visceral involvement. Diagnosis was based on histopathological examination of needle biopsied material. The patient was successfully treated with sodium stibogluconate as shown clinically and parasitologically. It was concluded that lymphatic leishmaniasis should be in mind when dealing with lymphanopathy of unknown aetiology.

Adult↗

PubMed1992

Cutaneous leishmaniasis and basal cell carcinoma in a patient from Al Baha, Saudi Arabia.

While cutaneous leishmaniasis (CL) is a protozoal parasite of the skin, basal cell carcinoma (BCC) is a malignant tumour of the skin. In this paper, we report the identification of cutaneous leishmaniasis and basal cell carcinoma in one patient, in the same site and same lesion. This raised the possibility that CL is a predisposing factor for BCC.

Adolescent↗

PubMed1992

The validity of AG-NOR profile in schistosomal lesions of the urinary bladder.

AG-NOR was determined in the nuclei of thirty-five urinary bladder lesions, varying between benign and malignant schistosomal lesions and non schistosomal ones. The results showed that the assessment of AG-NOR in urinary bladder lesions can be valuable in distinguishing benign from malignant lesions of the urinary bladder and can be used as a prognostic parameter.

Adult↗

PubMed1992

Histopathological study of the bilharzial affection on the bladder and ureter.

This study was carried out on 68 cases (50 bilharzial stricture lower ureter, eight vesico-ureteral reflux cases and ten control). In cases of reflux associated with bilharziasis heavy ova-deposition was obvious all through the ureteral wall especially per-ureteral sheath. Also heavy deposition in the bladder wall involved the detrusor and trigonal muscle with fibrosis and obliteration of Waldeyer's space and atrophy and fasciculation of detrusor and trigonal muscle. This implies ureteral rigidity, impaired mobility and compression against a firm detrusor buttress.

Animals↗

PubMed1991

Detrusor morphology and pathology in relation to bladder outflow obstruction in bilharzial patients.

The light and electron microscopic structure of detrusor muscle of trabeculated urinary bladder from patients with urodynamically proved bilharzial outflow obstruction had been compared with normal detrusor muscle. In bilharzial outflow obstruction, the detrusor muscle bundles were formed of relatively smaller smooth muscle cells widely separated by dense connective tissue, fatty infiltration, bilharzial ova, inflammatory cells and hyalinosis. No evidence of smooth muscle hyperplasia, mitosis, or fibroblastic proliferation. At the level of electron microscopy. The connective tissue infiltration was proved to be microfibriles apparently in continuity of the basal lamina of the smooth muscle. These pathological changes can explain both the morphological and urodynamic changes in bilharzial urinary bladder outflow obstruction.

Adult↗

PubMed1991

Diagnostic value of DNA content in keratosis and adult papilloma of the larynx.

Nuclear DNA content of laryngeal epithelial cells in smears was carried out by DNA cytometry with automatic microscope and T.V. image analysis system in 6 adult papilloma and 10 keratosis of the larynx patients. The method had high degree of precision confirmed by whole tissue biopsy and serial sections. The method is non invasive and allows fair diagnosis of suspicious and early malignant cases.

Adult↗

PubMed1991

Extruded and protruded lumbar discs: combined clinical, histopathological, histochemical, immunopathological, histochemical, immunopathological and ultrastructural studies.

A total number of selected 252 patients with prolapsed lumbar intervertebral discs (92 extruded and 160 protruded) were operated upon in Neurosurgery Department Zagazig University Hospital during the period extended from January, 1988, to October 1990. In this study we reviewed their clinical and operative data. Surgical biopsies were taken from randomly selected 120 patients of them (50 extruded and 70 protruded discs) and were subjected to histopathological, histochemical and immunopathological studies. Ultrastructural study was performed for randomly selected 14 cases (7 extruded and 7 protruded discs). The results were compared with 7 normal intervertebral discs obtained from these studies showed that there were no differences between extruded and protruded discs at the histochemical level. Cell mediated immunity could be added as a factor in the pathogenesis of the degenerative process that lead to disc prolapse (in 20% of cases with extruded discs and 57.1% of cases with protruded discs). At the ultrastructural level 85.3% of extruded discs were prolapsed nucleus pulposus while 14.7% were prolapsed annulus fibrosus alone or with nucleus pulposus. All protruded discs were prolapsed annulus fibrosus with nucleus pulposus. These findings may have an impact on the management of this common problem.

Adult↗

PubMed1991

Causes of lymphadenopathy in Egyptian children other than malignancy.

Fifty children aged 3 to 12 years presented with cervical or inguinal lymph nodes enlargement were selected from Zagazig University Hospitals. They were subjected to routine laboratory examination as well as histopathological & immunopathological studies. The results revealed eosinophilia and intestinal parasites in 80 percent. Besides, pediculosis and flea dermatitis were also demonstrated in 30 and 12 percent respectively. Histopathological studies of the nodal tissues revealed sinus hyperplasia (ten cases), reactive paracortical hyperplasia (30 cases) and necrosis and caseition (ten cases). Infiltration with eosinophils was demonstrated in the nodal tissues of 40 cases. Immunopathologyical studies revealed cytotoxic T-cells in all the cases, and helper T lymphocytes in only 15 cases. It is concluded that this may give an explanation of some causes of lymphadenopathy in Egyptian children other than malignancy.

Biopsy↗

PubMed1991

Schistosomiasis affection of the kidney as a possible cause of renal stone formation.

Nowadays, renal stone is one of the medical complaints. Many theories have been suggested to account for its formation. The present work aimed to find out a correlation between schistosomiasis affection of the kidney and the formation of renal stones. Three groups of patients with renal stones were selected. They had Schistosoma mansoni infection (1st group), with Schistosoma haematobuim infection (2nd group) and Schistosoma free (3rd group or control). The results showed pathological changes in the kidney biopsied materials, particularly the glomerulus, ranging from periglomerular fibrosis to complete atrophy and lymphoid follicles in the interstitium. Sometimes, distal-tubular atrophy was seen. The serum and tissue immunoglobulins, particularly the IgA, in schistosomiasis patients were above normal as compared to control ones. It is concluded that the schistosomiasis affection of the kidney, and the resulting immunopathological changes were factors predisposing, in one way or another, to the formation of renal stones.

Adult↗

PubMed1991

Pathological and immunopathological changes in albino mice experimentally infected with Hymenolepis nana.

The pathological and immunopathological effects of H. nana on experimentally infected albino mice were studied, sacrificed one, three and five months post egg inoculation. The results showed that most of the mice had diarrhoea, loss of appetite and were inactive. On the other hand, the liver and spleen showed some pathological changes and cysticercoids were seen particularly in the liver. The kidneys showed cloudy swelling (3/90). The brain showed oedema (15/90) and glyosis (15/90). Intestinal atrophy and ulceration were very marked (30/90) with negative reaction to PAS and Alcian blue stain. Cysticercoids were seen in intestinal villi. The immunoglobulins in sections of the S. intestine changed from moderate to negative (IgA) or to mild (IgM) or from mild to marked (IgG). The whole results were discussed.

Animals↗

PubMed1990

Schistosomiasis associated bladder carcinoma expression of cytokeratin.

Twenty patients with bladder carcinoma (14 transitional cell carcinoma and six squamous cell carcinoma) and ten controls of (four with bilharzial lesions, three without and three with metaplasia and dysplasia) were subjected to immunocytochemical and immunohistochemical studies. Bladder and urine samples examination were carried out with the high molecular weight cytokeratin and broad spectrum keratin antibodies using peroxidase anti-peroxidase. Cytokeratins of high molecular weight were expressed in all squamous cancer while broad spectrum keratin was positive in 83.3%. The transitional cell carcinoma was positive in 50% with both cytokeratin and broad spectrum keratin. Urine shedded were positive in 83.3% and 66.7% of squamous cell carcinoma with high molecular weight cytokeratin and broad spectrum keratin respectively. However, each of them was positive in 50% of transitional carcinoma, and negative with non-malignant cases except with metaplasia and dysplasia.

Biomarkers, Tumor↗

PubMed1990

Bilharzial role in staging bladder carcinoma histopathological-transurethral sonography and computerized tomography.

Bilharzial bladder tumour of 28 patients staged pre-operatively by transurethral ultrasound and computerized tomography (CT). The accuracy varied from 66.6% to 77.7% in different stages as compared with pathological stage. The bilharzial reaction could be play a role in this accuracy and the histopathological study is the accurate way to express the depth of infiltration of the tumour in the bladder wall as well as the depth of vascular invasion which may be greater than the primary tumour itself.

Adult↗

PubMed1990

Pathologic examination of the placenta in human cases of toxoplasmosis.

One hundred sixty one placentae were examined grossly and microscopically, 131 specimens belonged to seropositive patients and 30 specimens belonged to seronegative patients and used as control. 63.3% of the placentae of seropositive patients were macroscopically normal, 9% were hydropic, 21% were of mixed or partially hydropic pattern, 6% had areas of infarction & 0.7% showed areas of calcifications. The most consistent microscopic finding was focal low grade chronic villitis with mononuclear inflammatory infiltrate. In hydropic placentae villous edema was of very variable degree from one placenta to another and from one area to another in the same placenta. Nucleated red blood cells were demonstrated in villous capillaries. The patients had Rh positive factor in their blood.

Abortion, Spontaneous↗

PubMed1989

The relation of HLA-DR antigen, lymphocytes and natural killer cells in human bilharzial bladder carcinoma.

The HLA-DR antigen type was studied in fifty-four patients with bilharzial bladder carcinoma and in ten patients but with non malignant bilharzial bladder lesions as controls. The monoclonal antibodies were used to identify the pattern of the distribution of lymphocytes subset and the natural killer cells. These immunohistochemical studies through some light on the relation between the different immunocompetent cells in the bladder tissue and suggested their functional role particularly in bilharziasis and in the tumour surveillance.

Antibodies, Monoclonal↗

PubMed1989

Vascular changes in cutaneous leishmaniasis.

Fifty cases of proven human cutaneous leishmaniasis were histopathologically studied. In twenty biopsies the picture was vascular alternations with or without fibrinoid necrosis and fibrin thrombi in both arteries and veins within the area of the inflammation in the lesions. Hyalinosis and intimal proliferation were also seen. On the other hand, immunoperoxidase staining of CL. lesions showed IgG and IgA within the endothelial cells, in the media and the perivascular space of the diseased vessels. It seems that the formation of immune complexes in the infected skin was responsible for the vascular changes.

Adult↗

PubMed1989

Evaluation of T cells, B cells and Ig. in tissues of human cutaneous leishmaniasis.

Cutaneous leishmaniasis (CL) is a granulomatous skin disease. The healing of the CL. lesions is attributed to the delayed immunity developed against the amastigotes of the parasite. However, in some cases in spite of the development of strong delayed hypersensitivity CL. lesions failed to heal. In this paper, it was intended to assess the role of immunity in the lesions of human cutaneous leishmaniasis. The results showed that the helper cells predominated the suppressor ones, the mean percentage of B lymphocytes was 25 and the immunoglobulins particularly the IgG were demonstrated by being positively stained. On the other hand, the complement (C3) was within the normal range.

B-Lymphocytes↗