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Biomedical subjects

A M Lee

Publications and source records attributed to A M Lee.

84 records · Page 5Linked to original sources
PubMed1978

Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli.

The Oxi/Ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. The nine biochemical reactions used as the initial tests in the Oxi/Ferm tube system showed a close agreement with the same conventional reactions. However, the system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distributed in 48 species and biotypes and included organisms not listed in the Oxi/Ferm tube identification list. The system correctly identified 56% of the test organisms when the degree of identification was based on the manufacturer's guidance list. However, all isolates of Acinetobacter calcoaceticus, Bordetella bronchiseptica, Pseudomonas aeruginosa, P. diminuta, and group IIf were completely identified by the system.

Bacteriological Techniques↗
PubMed1977

Relationship between birth weight and perceptual motor performance in children.

A perceptual-motor performance test (jumping, skipping, balancing, walking) was administered to 150 5- and 6-yr.-old children categorized according to birth weight. Differences were found among the three birth-weight groups, with the children of low birth weight scoring significantly lower than the groups of medium and high birth weight. A substantial positive correlation of .75 was obtained between birth weight and the perceptual-motor performance (total number of items passed).

Birth Weight↗
PubMed1977

A scheme for identification of nonfermentative gram-negative bacteria.

A scheme was designed to speed the identification of 50 nonfermentative gram-negative bacteria to genus, species, and biotype using a minimal numer of biochemical tests. When the scheme was evaluated in our laboratory, one technologist identified 93.6% of 188 known isolates (46 species). Later another technologist, given 161 of the same isolates (46 species) as unknowns, identified 91.9%. The isolates that were misidentified in both cases were atypical organisms with aberrant biochemical reactions, plus 1 typical organism with a delayed oxidation of glucose.

Bacteriological Techniques↗
PubMed1968

A method for the formulation of influenza virus vaccine using numerical taxonomy.

The relationship of new "field" isolates of influenza virus to previously isolated strains might not be evident by inspection of haemagglutination-inhibition data. Numerical taxonomic methods have been applied to experimental results in order to clarify these relationships so that antigenic trends may be more easily seen. The diagrammatic representations so derived suggest that numerical taxonomic methods may be useful in the future in selecting the most representative influenza virus strains for inclusion in a vaccine.

Antigen-Antibody Reactions↗
PubMedDate not supplied

The International Space Station human life sciences experiment implementation process.

The selection, definition, and development phases of a Life Sciences flight research experiment has been consistent throughout the past decade. The implementation process, however, has changed significantly within the past two years. This change is driven primarily by the shift from highly integrated, dedicated research missions on platforms with well defined processes to self contained experiments with stand alone operations on platforms which are being concurrently designed. For experiments manifested on the International Space Station (ISS) and/or on short duration missions, the more modular, streamlined, and independent the individual experiment is, the more likely it is to be successfully implemented before the ISS assembly is completed. During the assembly phase of the ISS, science operations are lower in priority than the construction of the station. After the station has been completed, it is expected that more resources will be available to perform research. The complexity of implementing investigations increases with the logistics needed to perform the experiment. Examples of logistics issues include- hardware unique to the experiment; large up and down mass and volume needs; access to crew and hardware during the ascent or descent phases; maintenance of hardware and supplies with a limited shelf life,- baseline data collection schedules with lengthy sessions or sessions close to the launch or landing; onboard stowage availability, particularly cold stowage; and extensive training where highly proficient skills must be maintained. As the ISS processes become better defined, experiment implementation will meet new challenges due to distributed management, on-orbit resource sharing, and adjustments to crew availability pre- and post-increment.

Biological Science Disciplines↗
PubMedDate not supplied

Dicoumarol toxicity in cattle associated with ingestion of silage containing sweet vernal grass (Anthoxanthum odoratum).

A diagnosis of dicoumarol toxicity in a herd of Friesian cattle was made following investigation of the deaths of three mature cows and eleven yearling heifers. Affected stock had been fed wrapped, bailed silage containing approximately 90% sweet vernal grass (Anthoxanthum odoratum). Sweet vernal grass contains coumarin, which can be converted to dicoumarol, a vitamin K antagonist, through the action of moulds. Most deaths were preceded by lethargy, severe anaemia and subcutaneous and internal haemorrhage. Dicoumarol toxicosis was suspected based on clinical signs, necropsy findings and prolonged prothrombin and activated partial thromboplastin times. Dicoumarol analysis of blood from affected animals and silage confirmed the diagnosis. Activated partial thromboplastin time Haemoglobin Packed cell volume Prothrombin time Red cell count

Animal Feed↗