Search PubMed⌕ Search

Biomedical subjects

A M Kuznetsov

Publications and source records attributed to A M Kuznetsov.

27 records · Page 2Linked to original sources

Analysis of river water by bioluminescent biotests.

The bacterial bioluminescence has high sensitivity to the action of various inhibitors of biological activity. The lyophilized luminous bacteria Photobacterium phosphoreum (Microbiosensor B17 677F) and luminous strain Escherichia coli (Microbiosensor EC) from the Culture Collection IBSO were used to create bioluminescent biotests. They have been applied in ecological monitoring to determine the overall toxicity of the Yenisei and Angara Rivers and some water sources of Altai Territory. As a rule the heaviest pollution of water in studied rivers was registered near cities and settlements. The luminous bacteria biotests are simple and convenient in work, standardized and quantitative, have rapid response to actions of different substances and high sensitivity to environmental pollutants. It takes less than 30 min to do the biotest (the other biotests take 48--96 h).

Biological Assay↗

[Antifungal activity of aqueous extracts from the leaf of cowparsnip and comfrey].

We found that extracts from the leaves of medicinal comfrey and cowparsnip strongly inhibit the germination of Erysiphe graminis conidia and uredospores of Puccinia graminis. Spraying wheat seedlings with these extracts, in contrast to the irrigation of soil, markedly diminished infection in plants with powdery mildew. Antifungal activity in vitro and protective activity (when plants were sprayed) correlated with the level of phenolic compounds in these extracts. Experiments with healthy plants have demonstrated that the photosynthetic apparatus of wheat plants is stimulated by extracts. Spraying seedlings with the extracts resulted in an increased rate of O2 evolution calculated per unit of chlorophyll, an increase in the ratio (FM-FT)/FT in the experiments that recorded slow fluorescence induction, an increase in the relative light intensity of band A, and a decrease of relative intensity of band C in experiments with thermoluminescence of wheat leaves. These results provide evidence that the protective activity of comfrey and cowparsnip extracts is associated with their action on the pathogenic fungus and with the activation of natural defense reactions of the host plant.

Antifungal Agents↗

[Possible use of new corrosion-resistant steels in the manufacture of medical instruments].

Demands on metals intended for the manufacture of medical instruments call for the use of corrosion-resisting grades of steel with high strength characteristics. Grades of steel of the martensite class reinforced by age hardening (martensive-aging) and mild grades of steel (chrome-manganese and chrome-nickel) have a number of valuable advantages over the steel of other classes. The possibility of obtaining high strength with low carbon dontent ensures great plasticity, toughness and brittle failure resistance along with corrosion-resistance and the possibility of achieving a substantial simplification in technology of making the instruments. Investigations of martensite-aging grades of steel--ep832,ochX11H9M2d2Tyu, O3X11HMd2yu and O3X13g3Md2 demostrated them to hold good promise for the manufacture of surgical and atraumatic needles, and rod-type stomatological instruments.

Dental Instruments↗